Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 681,564 C→G 4.3% T681T (ACG→ACG)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034681,5640C→G 4.3% 9.4 ‑0.8 ‑0.9163T681T (ACG→ACC) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (5/2):  ref base (83/72):  total (89/74)
Fisher's exact test strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.34e-01

Only 100 of 182 total aligned reads displayed.

CGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGG  >  NC_014034/681465‑681664
                                                                                                   |                                                                                                    
cGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGccgccg                                                                                                     >  1:786151/1‑101 (MQ=255)
 gTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGccgccgg                                                                                                    >  2:2128132/1‑100 (MQ=255)
 gTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGt                                                                                                    >  2:2308907/1‑101 (MQ=255)
  tGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTg                                                                                                   >  2:4161584/1‑101 (MQ=255)
   gCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGc                                                                                                  <  1:4405694/101‑1 (MQ=255)
   gCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGc                                                                                                  >  2:3647900/1‑101 (MQ=255)
      ggTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCa                                                                                               >  1:4339994/1‑101 (MQ=255)
      ggTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCa                                                                                               <  2:3574863/101‑1 (MQ=255)
        tCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgc                                                                                             <  1:685771/101‑1 (MQ=255)
        tCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgc                                                                                             <  1:4161094/101‑1 (MQ=255)
        tCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgc                                                                                             <  1:2101243/101‑1 (MQ=255)
          aGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgcgc                                                                                           >  2:2347894/1‑101 (MQ=255)
              cgcCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCgccccc                                                                                       >  2:636821/1‑101 (MQ=255)
               gcCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCa                                                                                      <  2:1567954/101‑1 (MQ=255)
                 cGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCAcc                                                                                    >  1:1226624/1‑101 (MQ=255)
                   aTCCGCGCCGAGCCCAGACGCGCCCCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGt                                                                                  <  1:1644537/101‑1 (MQ=255)
                      tGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCCGCCCCGCCCCCGTCGc                                                                               >  1:1046853/1‑101 (MQ=255)
                      tGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGc                                                                               <  2:3125059/101‑1 (MQ=255)
                      tGCGCCGAGCCCAGACGCGCCGCCTGCCCCGTCGTCAGCTCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGc                                                                               <  2:3688540/101‑1 (MQ=255)
                        cgcCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCg                                                                             >  2:4029198/1‑101 (MQ=255)
                         gcCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGa                                                                            <  2:3497503/101‑1 (MQ=255)
                          ccGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGaa                                                                           <  1:3567722/101‑1 (MQ=255)
                           cGAGCCCAGGCGCGCCGCCTGCGCGGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAg                                                                          <  2:941831/101‑1 (MQ=255)
                           cGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAg                                                                          <  2:4169980/101‑1 (MQ=255)
                           cGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAg                                                                          <  1:2523802/101‑1 (MQ=255)
                            gAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGc                                                                         >  1:3406452/1‑101 (MQ=255)
                             aGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGcc                                                                        <  1:4058288/101‑1 (MQ=255)
                               cccAGACGCGCCGCGTGCTCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAg                                                                      <  2:1412208/101‑1 (MQ=255)
                                ccAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAgc                                                                     <  1:2952995/101‑1 (MQ=255)
                                ccAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAgc                                                                     <  1:227247/101‑1 (MQ=255)
                                  aGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAgcgc                                                                   >  1:1736439/1‑101 (MQ=255)
                                   gACGCGCCCCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGcgcc                                                                  <  1:1077993/101‑1 (MQ=255)
                                    aCGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGGGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGcgccg                                                                 >  2:716535/1‑101 (MQ=255)
                                      ccgccgcCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGcgccgca                                                               <  1:3566650/100‑2 (MQ=25)
                                        gccgccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGcgccgcaag                                                             >  2:1748395/1‑98 (MQ=25)
                                        gccgccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGAt                                                             >  2:227697/1‑101 (MQ=255)
                                         ccgccTGCCCCGTCGTCAGCGCCACGATGGCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATc                                                            <  2:2054681/101‑1 (MQ=255)
                                          cgccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCt                                                           <  1:4206217/101‑1 (MQ=255)
                                          cgccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCt                                                           <  1:3251154/101‑1 (MQ=255)
                                           gccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTg                                                          >  1:3640733/1‑101 (MQ=255)
                                           gccTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTg                                                          >  2:1219999/1‑101 (MQ=255)
                                             cTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTgcg                                                        <  2:1736495/101‑1 (MQ=255)
                                             cTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTgcg                                                        <  2:2873621/101‑1 (MQ=255)
                                              tGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTgcgc                                                       <  2:1595102/101‑1 (MQ=255)
                                              tGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTgcgc                                                       >  1:2502967/1‑101 (MQ=255)
                                               gcgcCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGGCGCCGAAGCCAGCGCCGCGATCTGCGcc                                                      <  2:686285/101‑1 (MQ=255)
                                               gatcggtggtcAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGcc                                                      <  2:4373723/93‑1 (MQ=255)
                                                cgcCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCTCCg                                                     >  1:1178403/1‑101 (MQ=255)
                                                  ccGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTg                                                   >  2:3880636/1‑101 (MQ=255)
                                                   cgtcgtCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGc                                                  >  1:3031839/1‑101 (MQ=255)
                                                   cgtcgtCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGc                                                  >  2:486234/1‑101 (MQ=255)
                                                    gtcgtcAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCt                                                 >  1:2840947/1‑101 (MQ=255)
                                                     tcgtcAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTc                                                <  1:2207369/101‑1 (MQ=255)
                                                      cgtcAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTcc                                               >  2:691028/1‑100 (MQ=255)
                                                      cgtcAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCa                                               >  1:3779551/1‑101 (MQ=255)
                                                      cgtcAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCa                                               >  1:2009159/1‑101 (MQ=255)
                                                      cgtcAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCa                                               <  2:4192471/101‑1 (MQ=255)
                                                         cAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAgcg                                            >  1:2690516/1‑101 (MQ=255)
                                                         cAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAgcg                                            >  2:49925/1‑101 (MQ=255)
                                                         cAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAgcg                                            <  2:112489/101‑1 (MQ=255)
                                                          aGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCCgcgc                                           >  1:398570/1‑101 (MQ=255)
                                                          aGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAgcgc                                           >  1:3332760/1‑101 (MQ=255)
                                                          aGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAgcgc                                           <  2:3332523/101‑1 (MQ=255)
                                                           gcgcCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGcgcc                                          <  1:1318374/101‑1 (MQ=255)
                                                              ccACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGcgccgcc                                       >  1:1531272/1‑101 (MQ=255)
                                                              ccACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGcgccgcc                                       <  1:4007466/101‑1 (MQ=255)
                                                                  gATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCt                                   >  2:963163/1‑101 (MQ=255)
                                                                  gATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCt                                   >  2:2019109/1‑101 (MQ=255)
                                                                  gATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCt                                   <  2:1626886/101‑1 (MQ=255)
                                                                  gATGTCATCGGTGCCAAAGCCGCGGAGCCCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTTCGCCGTGCTCAGCGCCGCCGCCt                                   <  2:2519267/101‑1 (MQ=255)
                                                                   aTGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTg                                  >  2:2582160/1‑101 (MQ=255)
                                                                   aTGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTg                                  >  1:2600220/1‑101 (MQ=255)
                                                                    tGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGAACCGCCCCCGTCGCCGAAGCCAGCGCCGCGCTCTCCGCCGTGCTCAGCGCCGCCGCCtcc                                 >  1:2751457/1‑99 (MQ=38)
                                                                       cATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCAcc                              >  2:1003088/1‑101 (MQ=255)
                                                                       cATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCAcc                              >  2:1966856/1‑101 (MQ=255)
                                                                        aTCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCg                             <  2:2422656/101‑1 (MQ=255)
                                                                          cGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAg                           >  2:2520539/1‑101 (MQ=255)
                                                                          cGGTCCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAg                           <  2:2009030/101‑1 (MQ=255)
                                                                           ggTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGc                          <  2:3406215/101‑1 (MQ=255)
                                                                           ggTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGc                          >  2:776302/1‑101 (MQ=255)
                                                                           ggTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGc                          >  2:549522/1‑101 (MQ=255)
                                                                           ggTGCCAAAGCCGCGGACCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCTGTGCTCAGCGCCGCCGCCTGCACCGAGc                          <  2:44738/101‑1 (MQ=255)
                                                                             tGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCa                        >  2:2176252/1‑101 (MQ=255)
                                                                              gCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCCCCGAGCCaa                       >  1:1978056/1‑101 (MQ=255)
                                                                               ccAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAg                      <  1:2309057/101‑1 (MQ=255)
                                                                               ccAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAg                      >  2:27554/1‑101 (MQ=255)
                                                                               ccAAAGCCGCGGATCCCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAg                      <  2:4211899/101‑1 (MQ=255)
                                                                               ccAAACCCGCGCACCCCCCCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAg                      <  2:1385842/101‑1 (MQ=37)
                                                                                 aaaGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGcc                    >  1:3654916/1‑101 (MQ=255)
                                                                                 aaaGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGcc                    >  1:185118/1‑101 (MQ=255)
                                                                                  aaGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGccc                   >  2:845972/1‑101 (MQ=255)
                                                                                    gCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCgg                 >  1:3543199/1‑101 (MQ=255)
                                                                                         ggATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCAcc            >  1:3934309/1‑101 (MQ=255)
                                                                                            tCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCa         <  1:4379167/101‑1 (MQ=255)
                                                                                              gccgccGTCCCCAGCGCCGCCCCCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAg       <  2:2137810/101‑1 (MQ=255)
                                                                                                 gccgTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCt    >  2:2269186/1‑101 (MQ=255)
                                                                                                 gccgTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCt    >  2:3229209/1‑101 (MQ=255)
                                                                                                 gccgTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCt    >  2:1150168/1‑101 (MQ=255)
                                                                                                  ccgTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTg   >  2:427042/1‑101 (MQ=255)
                                                                                                   cgTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTgg  <  2:71673/101‑1 (MQ=255)
                                                                                                   |                                                                                                    
CGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGG  >  NC_014034/681465‑681664

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG