Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 681,666 G→C 7.1% L647L (CTC→GCC)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034681,6660G→C 7.1% 30.1 ‑2.2 ‑0.7210L647L (CTC→CTG) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (11/4):  ref base (96/99):  total (107/103)
Fisher's exact test strand distribution p-value = 1.06e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.98e-01

Only 100 of 222 total aligned reads displayed.

TGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCG  >  NC_014034/681566‑681766
                                                                                                    |                                                                                                    
   ccAGCGCCGCCCCCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAgc                                                                                                   <  2:4219558/101‑1 (MQ=255)
        gccgccACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGcc                                                                                              >  1:408965/1‑101 (MQ=255)
        gccgccACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGcc                                                                                              >  1:3127803/1‑101 (MQ=255)
         ccgccACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCg                                                                                             >  1:1058294/1‑101 (MQ=255)
         ccgccACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCg                                                                                             <  2:1977927/101‑1 (MQ=255)
            ccACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGAcc                                                                                          >  1:50991/1‑101 (MQ=255)
             cACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCt                                                                                         >  1:126622/1‑101 (MQ=255)
             cACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCt                                                                                         <  1:3915824/101‑1 (MQ=255)
              cccGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTg                                                                                        <  2:3542951/100‑1 (MQ=255)
              aCCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACATg                                                                                        >  2:1370026/1‑101 (MQ=255)
                cGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGAt                                                                                      <  1:2216846/101‑1 (MQ=255)
                cGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGAt                                                                                      >  2:905999/1‑101 (MQ=255)
                 gTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATc                                                                                     >  1:4349562/1‑101 (MQ=255)
                 gTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGACACCGCAAGGCTGGAGAGCGCGCCGACCTGATc                                                                                     >  1:3793731/1‑101 (MQ=255)
                  tCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCg                                                                                    >  2:4272759/1‑101 (MQ=255)
                    gCCGAGGCCAGCGCCGCGATCTGCGCCGTCCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCCCCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGt                                                                                  <  1:636889/101‑1 (MQ=255)
                    gCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGt                                                                                  >  1:2802099/1‑101 (MQ=255)
                     cggtggcaatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTg                                                                                 <  1:2151101/91‑1 (MQ=2)
                     cggtggcaatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTg                                                                                 <  1:4265343/91‑1 (MQ=2)
                     cggtggcaatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTg                                                                                 >  1:2926653/11‑101 (MQ=2)
                     ccGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTg                                                                                 <  2:2831848/101‑1 (MQ=255)
                       gtggcaatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGcc                                                                               >  2:1615103/9‑100 (MQ=2)
                        aaGCCAGCGCCGCGATCGGCGCCGTGCCCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTc                                                                              <  1:404575/101‑1 (MQ=255)
                         aGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCa                                                                             <  1:909055/101‑1 (MQ=255)
                           caatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAga                                                                           >  2:507259/5‑100 (MQ=2)
                            cAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCt                                                                          <  2:2434592/101‑1 (MQ=255)
                            cAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCt                                                                          >  1:3153217/1‑101 (MQ=255)
                            aatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgac                                                                          >  1:3198048/4‑99 (MQ=2)
                            aatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgac                                                                          >  1:189717/4‑99 (MQ=2)
                            aatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgac                                                                          >  2:3972218/4‑99 (MQ=2)
                             aGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCtt                                                                         >  1:165149/1‑101 (MQ=255)
                              gcgcCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTg                                                                        <  2:576832/101‑1 (MQ=255)
                              gcgcCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTg                                                                        <  1:175158/101‑1 (MQ=255)
                              gcgcCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTg                                                                        <  2:3942211/101‑1 (MQ=255)
                               cgccgcGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGc                                                                       >  2:2909260/1‑101 (MQ=255)
                               cgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgactgg                                                                       >  1:1012766/1‑96 (MQ=2)
                                gccgcGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGcc                                                                      <  1:963248/101‑1 (MQ=255)
                                gccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgactggc                                                                      >  1:373812/1‑95 (MQ=2)
                                 ccgcGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCg                                                                     <  1:1370123/101‑1 (MQ=255)
                                 ccgcGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCg                                                                     >  1:3032558/1‑101 (MQ=255)
                                  cgcgATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGa                                                                    >  1:2678484/1‑101 (MQ=255)
                                  cgcgATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGa                                                                    >  2:2362687/1‑101 (MQ=255)
                                     gATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCt                                                                 <  2:3640480/101‑1 (MQ=255)
                                      gtCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTg                                                                <  2:2621157/100‑1 (MQ=255)
                                      aTCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTg                                                                >  2:2959959/1‑101 (MQ=255)
                                        cTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCg                                                              >  1:3334101/1‑101 (MQ=255)
                                            gcCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTcgc                                                          <  1:4142411/101‑1 (MQ=255)
                                            gcCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTcgc                                                          >  2:3154001/1‑101 (MQ=255)
                                             ccGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTcgcg                                                         >  2:22907/1‑101 (MQ=255)
                                              cGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCgcgc                                                        >  2:3071680/1‑101 (MQ=255)
                                              cGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCGCAGCTTGCCGATCTGCGTCgcgc                                                        >  1:4374512/1‑101 (MQ=255)
                                               gTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCt                                                       <  2:1178312/101‑1 (MQ=255)
                                                 gCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCa                                                     <  2:2678312/101‑1 (MQ=255)
                                                  atCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAg                                                    <  2:100113/100‑1 (MQ=255)
                                                    cAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGc                                                  >  1:4188124/1‑101 (MQ=255)
                                                      gcgcCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGccc                                                >  2:3303760/1‑101 (MQ=255)
                                                       cgccgccgcCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCg                                               <  2:126601/101‑1 (MQ=255)
                                                       cgccgccgcCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCg                                               >  2:1855064/1‑101 (MQ=255)
                                                         ccgccgccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGaa                                             <  2:2801893/101‑1 (MQ=255)
                                                          cgccgccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGaaa                                            <  1:1855188/101‑1 (MQ=255)
                                                          cgccgccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGaaa                                            <  1:861607/101‑1 (MQ=255)
                                                           gccgccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAc                                           >  1:3615953/1‑101 (MQ=255)
                                                           cccgccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAc                                           <  2:4349291/100‑1 (MQ=255)
                                                             cgccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAccg                                         >  1:2417080/1‑101 (MQ=255)
                                                              gccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAccgc                                        <  2:2038838/101‑1 (MQ=255)
                                                              gccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAccgc                                        >  2:2162072/1‑101 (MQ=255)
                                                               ccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAccgcc                                       >  1:14043/1‑101 (MQ=255)
                                                                cTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAccgccg                                      >  2:2238031/1‑101 (MQ=255)
                                                                cTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAccgccg                                      >  2:3602740/1‑101 (MQ=255)
                                                                cTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAccgccc                                      >  1:550393/1‑100 (MQ=255)
                                                                  gCACCGAGCTAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCTGCTTGCCGATCTGCTTCGCGCTCAGGCCCGAAACCGCcgcg                                    >  2:4421338/1‑101 (MQ=255)
                                                                  gCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCcgcg                                    <  2:1736328/101‑1 (MQ=255)
                                                                    actgAGCCAAGCCCGGCCTCCGTAAGGCTGGAGAGCGCGCCGACCTGATCGGTACTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCt                                  <  2:758709/98‑1 (MQ=255)
                                                                    aCCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCt                                  <  2:408925/101‑1 (MQ=255)
                                                                     ccGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTg                                 <  1:716615/101‑1 (MQ=255)
                                                                         gCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCa                             >  1:4444587/1‑101 (MQ=255)
                                                                          ccAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAg                            <  2:610323/101‑1 (MQ=255)
                                                                          ccAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAg                            <  1:1966985/101‑1 (MQ=255)
                                                                           cAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGc                           >  1:3856095/1‑101 (MQ=255)
                                                                            aaGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGcc                          >  1:3403185/1‑101 (MQ=255)
                                                                            aaGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGcc                          <  2:2971293/101‑1 (MQ=255)
                                                                             aGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGccc                         <  2:3032337/101‑1 (MQ=255)
                                                                             aGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGccc                         >  2:2576306/1‑101 (MQ=255)
                                                                             aGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGccc                         <  1:2293837/101‑1 (MQ=255)
                                                                                  ggCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGa                    <  1:4029455/101‑1 (MQ=255)
                                                                                  ggCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGa                    >  1:3241722/1‑101 (MQ=255)
                                                                                     cACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCCAAACCGCCGCGCCGCCCAGCCCCCCGaccc                 >  2:3001556/1‑98 (MQ=255)
                                                                                      aCCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTg                >  2:2707788/1‑101 (MQ=255)
                                                                                       ccGCAAGGCTGGCGAGCGGGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTgc               <  2:1458253/101‑1 (MQ=255)
                                                                                       ccGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTgc               >  1:4226784/1‑101 (MQ=255)
                                                                                        cGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTgcg              <  1:2128271/101‑1 (MQ=255)
                                                                                         gCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTgcgc             >  1:1000559/1‑101 (MQ=255)
                                                                                          cAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGcc            <  1:1541733/101‑1 (MQ=255)
                                                                                          cAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGcc            <  1:1150259/101‑1 (MQ=255)
                                                                                           aaGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCg           >  1:2745219/1‑101 (MQ=255)
                                                                                           aaGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCAAGCCCCGCGATCTGCGCCg           >  2:1563440/1‑101 (MQ=255)
                                                                                            aGGCTGGAGAGAGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGt          <  1:2019238/101‑1 (MQ=255)
                                                                                             ggCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTg         >  2:3055808/1‑101 (MQ=255)
                                                                                                    gagCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCg  >  1:3576113/1‑101 (MQ=255)
                                                                                                    gagCGCGCCGACCTGATCGGTCCTCAGCTGGCCGATCTGCGCCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCGGCGCTCTGCCCCGGGCCCAGCg  >  2:3884819/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
TGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCG  >  NC_014034/681566‑681766

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG