Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	797,964	G→C	100%	G183G (GGG→GGC)	sucB →	dihydrolipoyllysine‑residue succinyltransferase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	797,964	0	G→C	100.0%	234.7	64	G183G (GGG→GGC)	sucB	dihydrolipoyllysine‑residue succinyltransferase
Reads supporting (aligned to +/- strand):  new base (12/52):  ref base (0/0):  total (12/52)

GAGGTTCCGGCCCCGGCGGCGGGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGGTCTGCCGCGGCTCC.AG.CCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCCGGCGGCGCCCGCGAAGGATGTCGAACATGCCCCCGCGGCGAAA  >  NC_014034/797866‑798060                                                                                                   |                                                                                                   gAGGTTCCGGCCCCGGCGGCGGGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTc                                                                                                  <  2:1244186/101‑1 (MQ=255) gAGGTTCCGGCCCCGGCGGCGGGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTc                                                                                                  <  1:3429877/101‑1 (MQ=255)  aGGTTCCGGCCCCGGCGGCGGGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCt                                                                                                 <  2:1867658/101‑1 (MQ=255)  aGGTTCCGGCCCCGGCGGCGGGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCt                                                                                                 <  2:2603004/101‑1 (MQ=255)  aGGTTCCGGCCCCGGCGGCGGGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCt                                                                                                 <  1:2143741/101‑1 (MQ=255)  aGGTTCCGGCCCCGGCGGCGGGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCt                                                                                                 <  2:3345203/101‑1 (MQ=255)   ggTTCCGGCCCCGGCGGCGGGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTg                                                                                                <  1:267970/101‑1 (MQ=255)   ggTTCCGGCCCCGGCGGCGGGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTg                                                                                                <  2:676205/101‑1 (MQ=255)     ttCCGGCCCCGGCGGCGGGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGcc                                                                                              <  2:3634098/101‑1 (MQ=255)      tCCGGCCCCGGCGGCGGGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCcg                                                                                             <  2:3536073/101‑1 (MQ=255)        cGGCCCCGGCGGCCGGCGTGCTGAGCGAGATCCGCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCcgcg                                                                                           <  1:3832268/101‑1 (MQ=255)              cggcggcggGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.a                                                                                    <  1:2921204/101‑1 (MQ=255)              cggcggcggGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.a                                                                                    <  1:127518/101‑1 (MQ=255)              cggcggcggGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.a                                                                                    <  1:2968249/101‑1 (MQ=255)                 cggcggGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.cc                                                                                <  1:2735905/101‑1 (MQ=255)                  ggcgggcgTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.ccc                                                                               <  1:2483517/101‑1 (MQ=255)                    cgggcgTGCTGAGCGAGNTCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCg                                                                             <  2:857166/101‑1 (MQ=255)                    cgggcgTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGGTCTGCCGCGGCTCC.CC.CCCCg                                                                             >  1:4321260/1‑101 (MQ=255)                    cgggcgTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCg                                                                             <  2:1916338/101‑1 (MQ=255)                     gggcgTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGGCGTCTGGGGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.cccccg                                                                            >  1:4163700/1‑99 (MQ=255)                      ggcgTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGc                                                                           <  1:757307/101‑1 (MQ=255)                        cgTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGGTCCCGGCGGCGGGGTCTGCCGCGGCTCC.AG.cccccggcg                                                                         >  2:924410/1‑96 (MQ=255)                        cgTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGCTCCTGGCGGCGGGCTCTGCCGCGGCTCC.CC.CCCCgggcc                                                                         >  1:3184222/1‑98 (MQ=255)                         gTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGc                                                                        <  1:1780263/101‑1 (MQ=255)                          tGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCt                                                                       <  1:2826635/101‑1 (MQ=255)                          tGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCt                                                                       <  2:2453609/101‑1 (MQ=255)                          tGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCt                                                                       <  2:3545450/101‑1 (MQ=255)                          tGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCt                                                                       <  1:1631284/101‑1 (MQ=255)                           gCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCTg                                                                      <  2:593365/101‑1 (MQ=255)                            cTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGGTCTGCCGCGGGGCCGGG.CCCCGGccccgc                                                                      >  1:2799776/1‑97 (MQ=255)                            cTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCTgc                                                                     <  2:1566484/101‑1 (MQ=255)                             tGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCTgcg                                                                    <  2:3020244/101‑1 (MQ=255)                              gAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCTgcgg                                                                   <  1:3233638/101‑1 (MQ=255)                              gAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCTgcgg                                                                   <  1:2349476/101‑1 (MQ=255)                               aGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGGCTGGGGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AC.CCCCCGCCGCCgcggc                                                                  >  1:1617678/1‑101 (MQ=255)                                gCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCTgcggcg                                                                 <  1:3928540/101‑1 (MQ=255)                                 cGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCCTCTGGGGATCCTGGCGGCGGGCTCTGCCGCGGGTCC.CGCCCCCGGCCGCGgcgccg                                                                 >  2:1586198/1‑101 (MQ=255)                                  gagaTCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCTGCggcggc                                                               <  2:4320991/101‑1 (MQ=255)                                   agaTCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCTGCGGCGgcc                                                              <  2:1416059/101‑1 (MQ=255)                                     aTCCTCGCCCCCGATGGCGCCTCGGTCGCGGCGTGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCTGCGGCGgccgc                                                            <  1:3848110/101‑1 (MQ=255)                                        cTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCTGCGGCGGCCGcccc                                                         <  1:1152937/101‑1 (MQ=255)                                        cTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCTGCGGCGGCCGcccc                                                         <  1:2731942/101‑1 (MQ=255)                                         tCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCTGCGGCGGCCGccccc                                                        <  2:2118930/101‑1 (MQ=255)                                          cGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCTGCGGCGGCCGCCCCcg                                                       <  2:467318/101‑1 (MQ=255)                                          cGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCTGCGGCGGCCGCCCCcg                                                       <  2:2799570/101‑1 (MQ=255)                                            cccccGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGGGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCCGCCGCCGCGGCGGCCCCCCCcccg                                                     >  1:1770341/1‑101 (MQ=255)                                            cccccGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCTGCGGCGGCCGCCCCcgcg                                                     <  1:2633427/101‑1 (MQ=255)                                             ccccGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCTGCGGCGGCCGCCCCCgcgc                                                    <  1:1250499/101‑1 (MQ=255)                                               ccGAGGGCGCCTCGGTCGCGGGGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCTGCGGCGGCCGCCCCCGcgccc                                                  <  1:1451706/101‑1 (MQ=255)                                               ccGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCTGCGGCGGCCGCCCCCGcgccc                                                  <  2:4114037/101‑1 (MQ=255)                                               ccGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCTGCGGCGGCCGCCCCCGcgccc                                                  <  1:2443241/101‑1 (MQ=255)                                               ccGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCTGCGGCGGCCGCCCCCGcgccc                                                  <  1:2430007/101‑1 (MQ=255)                                               ccGAGGCCGCCTCGGTCGCGGGGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCTGCGGCGGCCGCCCCCGcgccc                                                  <  1:1586304/101‑1 (MQ=255)                                                cGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCTGCGGCGGCCGCCCCCGcgcccg                                                 <  2:2879989/101‑1 (MQ=255)                                                cGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCTGCGGCGGCCGCCCCCGcgcccg                                                 <  1:230067/101‑1 (MQ=255)                                                 gAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCTGCGGCGGCCGCCCCCGcgcccgc                                                <  2:2515453/101‑1 (MQ=255)                                                   gggCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCTGCGGCGGCCGCCCCCGCgcccgccc                                              <  2:2785331/101‑1 (MQ=255)                                                   gggCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCTGCGGCGGCCGCCCCCGCgcccgccc                                              <  2:1617572/101‑1 (MQ=255)                                                   gggCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCTGCGGCGGCCGCCCCCGCgcccgccc                                              <  1:2942646/101‑1 (MQ=255)                                                       gcCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCcggc                                          <  2:641677/101‑1 (MQ=255)                                                            ggTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCCGCCGCTGCGGCGGCCGCCCCCGCGCCCCCCCCCGCGgcgc                                     >  2:4352923/1‑101 (MQ=255)                                                                    cgggcggTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCCGGCGGCGCCCGCGAAg                             <  2:3183995/101‑1 (MQ=255)                                                                     gggcggTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCTGCGGCGGGCGCCCCCGCGCCCGCCCCGGCGGGGCCCGCGAAgg                            >  1:3145174/1‑101 (MQ=255)                                                                     gggcggTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCCGGCGGCGCCCGCGCAgg                            >  1:1771499/1‑101 (MQ=255)                                                                         ggTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCCGGCGGCGCCCGCGAAGGAGGt                        >  2:201265/1‑101 (MQ=255)                                                                               ggggCGACCCGGGCGGGGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCCGGCGGCGCCCGCGAAGGATGTAGAACa                  <  1:924495/99‑1 (MQ=255)                                                                               cTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCCGGCGGCGCCCGCGAAGGATGTCGAACa                  >  1:1245907/1‑101 (MQ=255)                                                                                 ggCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.GCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCCGGCGGGCCCCGCGAAGGATGTCCAACATg                >  2:2972113/1‑101 (MQ=255)                                                                                 ggCGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCCGGCGGCGCCCGCGAAGGATGTCGAACATg                >  2:997083/1‑101 (MQ=255)                                                                                   cGATCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCAGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCCGGCGGCGCCCGCGAAGGATGGCGAACATGcc              >  1:3229066/1‑101 (MQ=255)                                                                                     aTCCTGGCGGCGGGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCCCGCGGCGCCCGCGAAGGATGTCGAACATGcccc            >  2:3220782/1‑101 (MQ=255)                                                                                              gcggGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCCGGCGGCGCCCGCGAAGGATGTCGAACATGCCCCCCCGGCGaa   >  2:712999/1‑101 (MQ=255)                                                                                               cggGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCCGGCGGCGCCCGCGAAGGATGTCGAACATGCCCCCGCGGCGaaa  >  1:1991384/1‑101 (MQ=255)                                                                                               cggGCTCTGCCGCGGCTCC.AG.CCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCCGGCGGCGCCCGCGAAGGATGTCGAACATGCCCCCGCCGCGaaa  >  1:17013/1‑101 (MQ=255)                                                                                                   |                                                                                                   GAGGTTCCGGCCCCGGCGGCGGGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGGTCTGCCGCGGCTCC.AG.CCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCCGGCGGCGCCCGCGAAGGATGTCGAACATGCCCCCGCGGCGAAA  >  NC_014034/797866‑798060 Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG