Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	900,796	C→G	100%	intergenic (‑125/‑162)	ldh ← / → ftsW	L‑lactate dehydrogenase/cell division protein FtsW

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	900,796	0	C→G	100.0%	61.7	28	intergenic (‑125/‑162)	ldh/ftsW	L‑lactate dehydrogenase/cell division protein FtsW
Reads supporting (aligned to +/- strand):  new base (22/6):  ref base (0/0):  total (22/6)

AACCCCGTTTCCG.CGCGG.CGCCGCTTTGCCGCAGCCCCGGTTTTCCTT.GGTGCAAATACGCCCGGGGGT...GC...CGCGGA..G.....CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTGGT  >  NC_014034/900725‑900870                                                                                 |                                                                                  acccccGTTCCCGCCGCGGCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTT.GGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCg                                                      <  1:1386333/99‑1 (MQ=12)  aCCCCGTTTCCGCCGCGGCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTT.GGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCgg                                                     >  1:52468/1‑101 (MQ=16)         ttCCGCCGCGGCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTT.GGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGccccagatcggaa                                              >  2:2033036/1‑92 (MQ=12)            cgccgcgGCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTT.GGTGCAAATACGCCGGGGGGTGGGGG...GGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCggg                                           <  1:3711040/98‑1 (MQ=12)               cgcgGCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTT.GGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCg                                        >  2:2532543/1‑101 (MQ=18)               cgcgGCCGCCGCTTTGCCGCAGCCCCGGTTTTCCTT.GGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCg                                        >  2:1380611/1‑101 (MQ=18)                  ggccgccgcTTTGCCGCAGCCCCGGTTTTCCTT.GGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGaaa                                     >  1:530998/4‑101 (MQ=18)                  ggccgccgcTTTGCCGCAGCCCCGGTTTTCCTT.GGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGaaa                                     >  1:825051/4‑101 (MQ=18)                   gccgccgcTTTGCCGCAGCCCCGGTTTTCCTT.GGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAg                                    >  2:242516/3‑101 (MQ=18)                       ccccTTCGCCGCACCCCCGGTTTTCCTG.GGTGCAAAGCCGCCGGGGGGT...GC...GGGGGC..GGCCCCCCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGcccc                                <  1:3515912/98‑1 (MQ=9)                        ccttttGCCCCAGCCCCGGTTTTCCTT.GGGGAAAAGCCGCCGGGGGGG...GCGGGGGCGGACCC.....CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGccccc                               <  2:1843460/98‑1 (MQ=2)                         gcTTTGCCGCAGCCCCGGTTTTCCTT.GGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCcg                              >  1:3673428/1‑101 (MQ=18)                           tttGCCGCAGCCCCGGTTTTCCTT.GGTGCAAATACGCCCGGGGGG...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCCGCCCTGGCGGGGCGAAAGCCCCcccg                            >  2:389094/1‑101 (MQ=16)                            ttGCCGCAGCCCCGGTTTTCCTT.GGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCcgcgc                           >  2:3370766/1‑101 (MQ=18)                            tggCCGCAGCCCCGGTTTCCCTG.GGTGCAAACGCGCCGGGGGGT...GG...GGGGGG..GGCCCCCCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCcgcgc                           <  1:4217616/99‑1 (MQ=9)                              gCCGCAGCCCCGGTTTTCCTT.GGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCgcgcgc                         >  2:2390018/1‑101 (MQ=18)                               ccGCCGCCCC.GTTTTCCTTGGGTGCAAATCCGCCGGGGGGG...GCGGGGGCGGACCC.....CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGcgcc                        <  1:2532706/101‑1 (MQ=2)                                ccgcgCCCCGTTTGTCCGT.GGCACAAATACGCCCGGGGGC...GCGGGGGCGGT..GCC...CCCCCGGCTCGCCCCGGCCCTGCCAGGGCGAAAGCCCCCGCGcgccg                       <  2:4052051/97‑1 (MQ=255)                                cGCAGCCCCGGTTTTCCTT.GGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGcgccg                       >  1:3153953/1‑101 (MQ=18)                                   aGCCCCGGTTTTCCTT.GGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCgg                    >  1:1520120/1‑101 (MQ=18)                                     ccccGGTTTTCCTT.GGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGcc                  >  1:1882378/1‑101 (MQ=18)                                         ggTTTTCCTT.GGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCTCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGc              >  1:2005599/1‑101 (MQ=18)                                          gTTTTCCTT.GGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCa             >  2:1260143/1‑101 (MQ=18)                                            tttCCTT.GGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCaaa           >  1:741605/1‑101 (MQ=18)                                             ttCCTT.GGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCaaaa          >  1:720277/1‑101 (MQ=18)                                              tCCTT.GGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAg         >  1:2216078/1‑101 (MQ=18)                                                cTT.GGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCCCGCGCCGCGGCCCGGCAAAAGct       >  1:4276648/1‑101 (MQ=18)                                                  tgggTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGctc      <  1:2758041/99‑1 (MQ=18)                                                  t.GGTGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGctct     >  1:2517135/1‑101 (MQ=18)                                                      tGCAAATACGCCCGGGGGT...GCGGGGGCGGA..GCC...CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTGGt  >  1:380081/1‑101 (MQ=18)                                                                                 |                                                                                  AACCCCGTTTCCG.CGCGG.CGCCGCTTTGCCGCAGCCCCGGTTTTCCTT.GGTGCAAATACGCCCGGGGGT...GC...CGCGGA..G.....CCCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTGGT  >  NC_014034/900725‑900870 Base quality scores:  ATCG  < 3 ≤  ATCG  < 10 ≤  ATCG  < 20 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG