Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	974,361	C→G	100%	intergenic (+241/+18)	RCAP_rcc00884 → / ← RCAP_rcc00885	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	974,361	0	C→G	100.0%	193.5	52	intergenic (+241/+18)	RCAP_rcc00884/RCAP_rcc00885	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (35/16):  ref base (0/1):  total (35/17)

GTGTGCGGAAGCCCCCGGCGGGTCGCCTCGGCGACAGCCGAGGCGAAATACCCCCGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAGCGCCC  >  NC_014034/974307‑974458                                                       |                                                                                                  cgggggcggaGCCCCCGGCGGGGGGCCCCCGGGGCAGCCGCGGGGGAAAAACCCCGCCGCAACGGCCGGGGCTCCCCCGAACAAGAACAGGGTCGAgcgcg                                                     >  1:109479/10‑101 (MQ=255)          ccgccggggcggGTCGCCTCGGCGACAGCCGAGACGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCAccc                                            <  1:3871408/94‑1 (MQ=255)          aGCCCCCGGCGGGTCGCCCCGGCGACAGCCCAGGCGGAAAACCCCCGCCCCAACCGCCGGGTCTCCGCCGGACAAGAACAGGGTCGGGCGCGGGGTCAccc                                            >  2:3370673/1‑101 (MQ=255)           ccccggggcggGTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCa                                           <  1:270024/95‑1 (MQ=255)            ccccgGGCGGGTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCag                                          <  2:2333981/101‑1 (MQ=255)                gggcggGTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAggg                                      <  1:2837748/100‑1 (MQ=255)                  gctggTCGCCCCGGCGACAGCGGAGGCGAAATCCCCCGGCCGCAACTGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGtc                                    <  2:2778433/98‑1 (MQ=255)                  gcggGTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGtc                                    >  1:3296373/1‑101 (MQ=255)                    gggTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGtcat                                  >  1:3322759/1‑101 (MQ=255)                    gggTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGtcat                                  >  1:709513/1‑101 (MQ=255)                     ggTCGCCTCGGCGACAGCCGAGGCGAAATCCCCTCGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGtcatc                                 <  1:3828001/101‑1 (MQ=255)                      gTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGtcatca                                >  1:3014007/1‑101 (MQ=255)                       tCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAg                               <  1:1916400/101‑1 (MQ=255)                        cGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAgc                              >  2:1825450/1‑101 (MQ=255)                        cGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAgc                              >  2:3957320/1‑101 (MQ=255)                          ccTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGGGCGCGATCACCCACAGGGTCATCAgcgc                            >  2:1872782/1‑101 (MQ=255)                           cTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCa                           >  1:3869061/1‑101 (MQ=255)                           cTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCa                           >  2:3689496/1‑101 (MQ=255)                           cTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCa                           >  1:3727378/1‑101 (MQ=255)                           cTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCa                           >  2:924632/1‑101 (MQ=255)                            tCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCAt                          <  1:2487130/101‑1 (MQ=255)                            tCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCAt                          >  1:2350302/1‑101 (MQ=255)                            ccGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCAt                          <  1:3470814/100‑1 (MQ=255)                             cGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATa                         >  1:1991960/1‑101 (MQ=255)                             cGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATa                         >  2:187029/1‑101 (MQ=255)                              ggCGACAGCCGAGGCGAAATCCCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAg                        <  2:1697433/101‑1 (MQ=255)                              ggCGACAGCCGAGGCGAAATCCCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAg                        <  2:1311325/101‑1 (MQ=255)                              ggCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAg                        >  2:2145181/1‑101 (MQ=255)                               gCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAgg                       >  2:577638/1‑101 (MQ=255)                               gCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAgg                       <  1:1552846/101‑1 (MQ=255)                               gCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAgg                       >  1:670139/1‑101 (MQ=255)                                cGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGc                      <  2:1455034/101‑1 (MQ=255)                                 gACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGcc                     >  1:1286125/1‑101 (MQ=255)                                 gACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGcc                     <  1:2070424/101‑1 (MQ=255)                                 gACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGcc                     >  1:935293/1‑101 (MQ=255)                                  aCAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCg                    >  2:3149873/1‑101 (MQ=255)                                  aCAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCg                    >  1:3039539/1‑101 (MQ=255)                                  aCAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGGTCGACCGCGCGCTCACCCAGAGGGACATCAGCGCATAGGCCg                    >  2:3031783/1‑101 (MQ=255)                                    aGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCa                  >  2:4031305/1‑101 (MQ=255)                                    aGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCa                  >  1:2367464/1‑101 (MQ=255)                                    aGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCa                  >  2:1652767/1‑101 (MQ=255)                                    aGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCa                  >  2:1357771/1‑101 (MQ=255)                                    aGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGCTCTCAGCCGAACAGGACCAGGTTTGGGCGCGCGATTACCGAGACGGTCATCAGCCCATAGCCCGCa                  >  2:1466836/1‑101 (MQ=255)                                     gCCGAGGCGAAATGCCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCaa                 <  1:2792446/101‑1 (MQ=255)                                      ccGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCaaa                >  2:3919374/1‑101 (MQ=255)                                      ccGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGGCATCAGCGCATAGGCCGCaaa                >  1:177364/1‑101 (MQ=255)                                       cGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAAc               >  1:2794319/1‑101 (MQ=255)                                       cGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAAc               <  2:3788734/101‑1 (MQ=255)                                         aGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAAccc             >  2:1477677/1‑101 (MQ=255)                                            cGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGc          >  1:553519/1‑101 (MQ=255)                                             gAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGcc         <  1:3518005/101‑1 (MQ=255)                                               aaTACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAg       >  1:454677/1‑101 (MQ=255)                                                aTACCTCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAgc      >  1:1286818/1‑101 (MQ=255)                                                 tcccccgGTCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAgcg     <  1:3850005/99‑1 (MQ=255)                                                 tcccccgGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAgcg     <  2:4064360/99‑1 (MQ=255)                                                  aCCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAgcgc    >  1:2296812/1‑101 (MQ=255)                                                    cccgGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAGCGccc  >  2:1255186/1‑101 (MQ=255)                                                       |                                                                                                  GTGTGCGGAAGCCCCCGGCGGGTCGCCTCGGCGACAGCCGAGGCGAAATACCCCCGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAGCGCCC  >  NC_014034/974307‑974458 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG