Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014035	102,786	C→G	14.9%	intergenic (‑45/+28)	RCAP_rcp00118 ← / ← RCAP_rcp00119	Na+‑dependent transporter/BioY family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014035	102,786	0	C→G	14.9%	2.5 ‑4.5 0.0	69	intergenic (‑45/+28)	RCAP_rcp00118/RCAP_rcp00119	Na+‑dependent transporter/BioY family protein
Reads supporting (aligned to +/- strand):  new base (12/1):  ref base (30/26):  total (42/27)
Fisher's exact test strand distribution p-value = 1.12e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

ATCGCCAGCAAAAGAAGCATGTAGGTGTCGATGCCGAAGCGTTTGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.CCGGCGGGGGAGGGGGCAGGGCGGTCCTCAGGCCGCGGTGGCGCCGCGGGCGCGGAGGATCGGCCAGGCCCGCTTGACCGACAGCGCGACCGCGGCGGTG  >  NC_014035/102686‑102886                                                                                                       |                                                                                                      aTCGCCAGCAAAAGAAGCATGTAGGTGTCGATGCCGAAGCGTTTGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGc                                                                                                       >  1:4174229/1‑101 (MQ=255)  tCGCCAGCAAAAGAAGCATGTAGGTGTCGATGCCGAAGCGTTTGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGc.c                                                                                                     <  1:3385043/101‑1 (MQ=255)  tCGCCAGCAAAAGAAGCATGTAGGTGTCGATGCCGAAGCGTTTGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGc.c                                                                                                     <  1:3048348/101‑1 (MQ=255)  tCGCCAGCAAAAGAAGCATGTAGGTGTCGATGCCGAAGCGTTTGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGc.c                                                                                                     <  1:2373162/101‑1 (MQ=255)   cGCCAGCAAAAGAAGCATGTAGGTGTCGATGCCGAAGCGTTTGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGg.cc                                                                                                    >  2:1594337/1‑101 (MQ=255)   cGCCAGCAAAAGAAGCATGTAGGTGTCGATGCCGAAGCGTTTGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGc.cc                                                                                                    <  1:2673925/101‑1 (MQ=255)   cGCCAGCAAAAGAAGCATGTAGGTGTCGATGCCGAAGCGTTTGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGc.cc                                                                                                    >  1:521898/1‑101 (MQ=255)    gCCAGCAAAAGAAGCATGTAGGTGTCGATGCCGAAGCGTTTGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGG.Ccg                                                                                                   >  1:3360231/1‑101 (MQ=255)     ccAGCAAAAGAAGCATGTAGGTGTCGATGCCGAAGCGTTTGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.Ccgg                                                                                                  <  1:4006709/101‑1 (MQ=255)      cAGCAAAAGAAGCATGTAGGTGTCGATGCCGAAGCGTTTGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGG.Cccgc                                                                                                 >  1:3510829/1‑101 (MQ=255)      cAGCAAAAGAAGCATGTAGGTGTCGATGCCGAAGCGTTTGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.Ccggc                                                                                                 <  2:171821/101‑1 (MQ=255)       aGCAAAAGAAGCATGTAGGTGTCGATGCCGAAGCGTTTGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGCGCcggc                                                                                                 >  2:313061/1‑101 (MQ=255)         cAAAAGAAGCATGTAGGTGTCGATGCCGAAGCGTTTGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.CCGGCggg                                                                                              <  1:849762/101‑1 (MQ=255)          aaaaGAAGCATGTAGGTGTCGATGCCGAAGCGTTTGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGG.CCGGGgggg                                                                                             >  1:1277764/1‑101 (MQ=255)          aaaaGAAGCATGTAGGTGTCGATGCCGAAGCGTTTGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGG.CCCGCgggg                                                                                             >  2:4087181/1‑101 (MQ=255)          aaaaGAAGCATGTAGGTGTCGATGCCGAAGCGTTTGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.CCGGCgggg                                                                                             <  2:3505893/101‑1 (MQ=255)           aaaGAAGCATGTAGGTGTCGATGCCGAAGCGTTTGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.GCGGCggggg                                                                                            >  2:3001659/1‑101 (MQ=255)           aaaGAAGCATGTAGGTGTCGATGCCGAAGCGTTTGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.CCGGCggggg                                                                                            <  2:3322610/101‑1 (MQ=255)            aagaagCATGTAGGTGTCGATGCCGAAGCGTTTGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.CCGGCGGGGGa                                                                                           <  1:1785227/101‑1 (MQ=255)                 gCATGTAGGTGTCGATGCCGAAGCGTTTGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.CCGGCGGGGGAggggg                                                                                      <  1:2091733/101‑1 (MQ=255)                  gaTGTAGGGGTCGAGGCCGAAGTGTTTGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGG.GGGC.CCGGCGGGGGAGGGGGc                                                                                     <  2:1758786/100‑1 (MQ=255)                  cATGTAGGTGTCGATGCCGAAGCGTTTGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGG.GCCGCGGGGGGgggggg                                                                                     >  2:2941686/1‑100 (MQ=255)                   aTGTAGGTGTCGATGCCGAAGCGTTTGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGG.CCCGCGGGGGGggggggg                                                                                    >  1:3304588/1‑99 (MQ=255)                   aTGTAGGTGTCGATGCCGAAGCGTTTGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.CCGGCGGGGGAGGGGGCa                                                                                    <  2:402969/101‑1 (MQ=255)                    tGTAGGTGTCGATGCCGAAGCGTTTGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGG.CCCGCGGGGGGgggggggg                                                                                   >  2:1564777/1‑98 (MQ=255)                    ggTAGGTGTCGATGCCGAAGCGTTAGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.CCGGCGGGGGAGGGGGCAg                                                                                   <  2:502443/100‑1 (MQ=255)                     gTAGGTGTCGATGCCGAAGCGTTTGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.CCGGCGGGGGGggggggggg                                                                                  >  1:2519371/1‑97 (MQ=255)                       gggTGTCGGTGCCGAACCTTTGGGGCGGGTTAATCTTTGGTCCTTCCCCGCAAGGGCG.GGCCCCCCCCTTGCGGG.GGCC.CCGGCGGGGGAGGGGGCAGGGc                                                                                <  1:288139/100‑1 (MQ=255)                       aGGTGTCGATGCCGAAGCGTTTGAGCGGGTTCATCTTTCGTCCTTTCACGCAAAAGCC.GGGCCCCTCCTTGAGGC.GGGG.CCGGAAGGGGAgggggggcggg                                                                                >  2:4205290/1‑95 (MQ=255)                        ggTGTCGATGCCGAAGCGTTTGAGGGGGTTCATCTTTCGCCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.CCGGCGGGGGAGGGGGCAGGGCg                                                                               <  1:315967/101‑1 (MQ=255)                        ggTGTCGATGCCGAAGCGTTTGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGG.CCGGCGGGGGAGGGGGCAGGGCg                                                                               <  1:585797/101‑1 (MQ=255)                          tgtCGATGCCGAAGCGTTTGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGG.GCGGCGGGGGGgggggggggggggg                                                                             >  1:2657379/1‑92 (MQ=255)                           gtCGATGCCGAAGCGTTTGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGCGGGGC.CCGGGGGGGGGgggggggggggggc                                                                             >  2:3878160/1‑92 (MQ=255)                             cGATGCCGAAGCGTTTGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.CCGGCGGGGGGGGGGGGGGGGCGGTTCt                                                                          >  1:1095268/1‑101 (MQ=255)                              gttGCCAAAGCGTTTGAGGTGTTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.CCGGCGGGGGAGGGGGCAGGGCGGTCCTc                                                                         <  2:3254599/99‑1 (MQ=255)                              gATGCCGAAGCGTTTGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGT.CCGGCGGGGGGGGGGGGGGGGGGGGCCTc                                                                         >  1:4377991/1‑101 (MQ=255)                                    gAAGCGTTTGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGG.CCGGCGGGGGGGGGGGGGGGGCGGGGCTCAGGCcg                                                                   >  1:2121367/1‑101 (MQ=255)                                      aGCGTTTGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGG.CCGGCGGGGGGGGGGGGAGGGGGGGGCCCCGGCcgcg                                                                 >  2:4118762/1‑101 (MQ=255)                                        gttttGGGCGGGTTCATTTTTCTTCCTTGCACGCAAGGGCG.GGGCCCCTCCTGGCGGG.GGGC.CCGGCGGGGGAGGGGGCAGGGCGGTCCTCAGGCCGCGGt                                                               <  1:4173337/99‑1 (MQ=255)                                        cGTTTGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.CCGGCGGGGGGGGGGGGCGGGCGGGGCTCACGGCGCggg                                                               >  2:2855028/1‑100 (MQ=255)                                          tttGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.CCCGCGGGGGGGGGGGGCGGGGGGGCCCCCGGCCGCGGGgg                                                             >  2:1005934/1‑101 (MQ=255)                                          tttGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.CCCGCGGGGGGGGGGGGAGGGGGGGCCCCCGGCCGCGGGgg                                                             >  2:2643772/1‑101 (MQ=255)                                           ttGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGG.CCGGCGGGGGGGGGGGGCGGGGGGGCCCCCGGGCGCGGGggg                                                            >  2:1341681/1‑100 (MQ=255)                                           ttGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.CCCGCGGGGGGGGGGGCAGGGCGGGCCTCAGGCCGCGGGggg                                                            >  2:2198912/1‑100 (MQ=255)                                           tggggCGGTTTCTTCTTCCTCCCTTCCCCCCAAGAGCC.GGCCCCCTCCTGGGGGC.GGGC.CGGGCGGGGGAGGGGGCAGGGCGGTCCTCAGGCCGCGGTGgc                                                            <  1:1129115/97‑1 (MQ=255)                                             gAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGG.CCCGGGGGGGGGGGGGGGGGGGGGGCCCCGCGGCGCGGGGgcgc                                                          >  1:2807662/1‑101 (MQ=255)                                               gCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.CCGGCGGGGGGGGGGGGGGGGCGGGCCTCCGGCCGCGGGgggggcg                                                        >  2:3087033/1‑96 (MQ=255)                                               gCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.CCGGCGGGGGGGGGGGCAGGGGGGGGCTCCGGCCGCGGGgggggcc                                                        >  2:2690762/1‑96 (MQ=255)                                                cGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGG.CCGGCGGGGGGGGGGGGGGGGGGGGGCTCAGGCCGCGGGGGcgccgc                                                       >  1:4086851/1‑101 (MQ=255)                                                cGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.CCGGCGGGGGGGGGGGGGGGGGGGGCCTCAGGCCCCCGGGGcggcgc                                                       >  1:2067751/1‑101 (MQ=255)                                                cGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.CCGGCGGGGGAGGGGGCAGGGCGGTCCTCAGGCCGCGGTGGcgccgc                                                       <  2:2519208/101‑1 (MQ=255)                                                 gggTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.CCGGCGGGGGAGGGGGCAGGGCGGTCCTCAGGCCGCGGTGGCGCcgcg                                                      <  1:2072187/101‑1 (MQ=255)                                                 gggTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.CCCGCGGGGGGGGGGGGGGGGGGGGCCCCCGGCCGCGGGGGGGCcgcg                                                      >  1:3051515/1‑101 (MQ=255)                                                 gggTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.CCCGCGGGGGGGGGGGGAGGGCGGGCCTCCGGCCGCGGGGGGGCcgcg                                                      >  1:1652791/1‑101 (MQ=255)                                                  ggTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.CCGGCGGGGGGGGGGGGCGGGGGGGCCTCCGGCCGCGGGGGGGCCGCgg                                                     >  1:3038866/1‑101 (MQ=255)                                                  ggTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.CCGGCGGGGGGGGGGGGAGGGGCGTCCTCCAGTCCCGGTGGgggcgccg                                                     >  2:1428336/1‑99 (MQ=255)                                                   gTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.CCCGGGGGGGGGGGGGGGGGGGGGGCCCCCCGCCGCGGGGGCGGCGCcgg                                                    >  1:2258961/1‑101 (MQ=255)                                                    ttCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGG.CCGGCGGGGGGGGGGGGGGGGGGGGCCTCCGGGCGCGGGGGGGGCGCgggg                                                   >  1:3777668/1‑100 (MQ=255)                                                    ttCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.CCGGCGGGGGGGGGGGGGGGGGGGGGCCCCGGCCGCGGGGGGGCCGCgggg                                                   >  1:882066/1‑100 (MQ=255)                                                    ttCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.CCGGCGGGGGGGGGGGGAGGGCGGGCCTCCCGCCGCGGGGGCGCCGCgggg                                                   >  1:1295676/1‑100 (MQ=255)                                                    ttCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.CCGGCGGGGGGGGGGGCAGGGGGGGCCTCAGGCCGCGGGGGCGCCGCGCgc                                                   >  2:126514/1‑101 (MQ=255)                                                     tCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.CCGGCGGGGGAGGGGGCAGGGCGGTCCTCAGGCCGCGGTGGCGCCGCGGgcg                                                  <  1:4088115/101‑1 (MQ=255)                                                       aTCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGG.CCGGCGGGGGGGGGGGGGGGGGGGGCCCCCGGCCCCGGGGGGGCCGCGGGggcg                                                >  2:4035287/1‑101 (MQ=255)                                                       aTCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGG.CCGGCGGGGGGGGGGGGCGGGGGGGGCCCAGGCCGCGGGGGGGCCGCGGGggcg                                                >  2:4441823/1‑101 (MQ=255)                                                        tCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.CCCGCGGGGGGGGGGGGGGGGGGGGCCCCCGGGCGCGGGGGCGCCGCGGGGGCgg                                               >  1:2467307/1‑101 (MQ=255)                                                         cTTTCCTCCTTTCCCG.AAGGGCCGGGGCCCCTCCTGGCGGC.GGGC.CGGGCGGGGGAGGGGGCAGGGCGGTCCTCAGGCCGCGGTGGCGCCGCGGGCTCgga                                              <  2:160659/101‑1 (MQ=255)                                                              gTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGG.CCCGCGGGGGGGGGGGGGGGGGGGGCCTCCGGCCGCGGGGGCGGCGCGGGCGCggcgggtg                                         >  2:1351314/1‑98 (MQ=255)                                                               tCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.CCGGCGGGGGGGGGGGGAGGGGGGGCCTCAGGCCGCGGGGGGGGCGCGGGCGCgggggggcg                                        >  2:3610388/1‑97 (MQ=255)                                                                   ttCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.CCCGCGGGGGGGGGGGGAGGGCGGGCCTCAGGCGCCGGGGGGGCCGCGGGGGCggggggggggccc                                    >  2:2567508/1‑93 (MQ=255)                                                                    tCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.CCGGGGGGGGAGGGGGGCGGGGGGGCCTCACGACGGGGGGGGGCCGGCGGCGGggggggttgggccg                                   >  1:1431646/1‑92 (MQ=255)                                                                     cACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGG.CCGGCGGGGGGGGGGGGAGGGGGGGCCTCCGGGCCCGGGGGGGCCCCGGGCGCgggggggcgggcacg                                  >  2:3495794/1‑91 (MQ=255)                                                                     cACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.CCGGGGGGGGGGGGGGCAGGGCGGTGCTCAGGCCGCGGGGGGGCCGCGGGGGCGGGGGGTCGGGCAgg                                  >  2:736157/1‑101 (MQ=255)                                                                     cACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.CCGGCGGGGGAGGGGGCAGGGCGGTCCTCAGGCCGCGGTGGCGCCGCGGGCGCGGAGGATCGGCCAgg                                  <  2:3359994/101‑1 (MQ=255)                                                                      aCGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.CCGGCGGGGGAGGGGGCAGGGCGGTCCTCAGGCCGCGGTGGCGCCGCGGGCGCGGAGGATCGGCCAGGc                                 <  1:760761/101‑1 (MQ=255)                                                                           agagCC.GGGCCCCTCCTTGCGGC.GGGC.CCGGGGGGGGGGGGGGGAGGGGGGGCCTCCGGCCGCGGGGGCGCCGCGGGGGCGGGGGGCCGGGCGGGCCCGcc                            >  1:2098978/1‑100 (MQ=255)                                                                            gagCC.AGGCCAATCCTGGCGGC.GGGC.CCGGCGGGGGAGGGGGCAGGGCGGTCCTCAGGCCGCGGTGGCGCCGCGGGCGCGGAGGATCGGCCAGGCCCGCtt                           <  2:4269980/101‑1 (MQ=255)                                                                             agCC.GGGCCCCTCCTTGCGGC.GGGC.CCGGCGGGGGGGGGGGGAGGGCGGGCCTCAGGCCGCGGTGGGGGCGCGGGGGCGGGGGGGCGGGCCGGCCCGCCTg                          >  2:573137/1‑101 (MQ=255)                                                                             agCC.GGGCCCCTCCTTGCGGC.GGGC.CCGGCGGGGGAGGGGGCAGGGCGGTCCTCAGGCCGCGGTGGCGCCGCGGGCGCGGAGGATCGGCCAGGCCCGCTTg                          <  1:2967594/101‑1 (MQ=255)                                                                              gCC.GGGCCCCTCCTTGCGGC.GGGC.CCGGCGGGGGGGGGGGGGGGGGGGGCCCCAGGCCGCGGGGGCGGCGCGGGCGCGGGGGAGCGGCCAGGCCCGCCTgg                         >  1:150312/1‑100 (MQ=255)                                                                               cc.GGGCCCCTCCTTGCGGC.GGGC.CCGGCGGGGGAGGGGGCAGGGCGGTCCTCAGGCCGCGGTGGCGCCGCGGGCGCGGAGGATCGGCCAGGCCCGCTTGAc                        <  2:2067614/101‑1 (MQ=255)                                                                                c.GGGCCCCTCCTTGCGGC.GGGC.CCGGCGGGGGGGGGGGGAGGGGGGGCCTCAGGCCGCGGGGGGGCCGCGGGGGCGGAGGGGCGGCCCGGGCCGCCTGaac                       >  2:919279/1‑99 (MQ=255)                                                                                c.GGGCCCCTCCTTGCGGC.GGGC.CCGGCGGGGGGGGGGGGAGGGGGGGCCCCAGGCCGCGGGGGGGCCGCGGGGGCGGGGGGTCGGGCCGGCCCGCGTGTcc                       >  2:1227732/1‑101 (MQ=255)                                                                                c.GGGCCCCTCCTTGCGGC.GGGC.CCGGCGGGGGGGGGGGGAGGGCGGGCCTCCGGCCGCGGGGGGGGCGCGGGCGGGGGGGGGCGGCCCGGGCCGCCTGGcc                       >  2:1659496/1‑101 (MQ=255)                                                                                  gggCCCCTCCTTGCGGC.GGGC.CCGGCGGGGGGGGGGGGAGGGGGGGCCTCAGGCCGCGGGGGCGCCGCGGGCGCGGGGGATCGGCCAGGCCCGCCTGAccc                      >  1:1429319/1‑100 (MQ=255)                                                                                  gggCCCCTCCTTGCGGC.GGGC.CCGGCGGGGGAGGGGGCAGGGCGGTCCTCAGGCCGCGGTGGCGCCGCGGGCGCGGAGGATCGGCCAGGCCCGCTTGACCg                      <  1:1314688/101‑1 (MQ=255)                                                                                     ccccCCCTGGGGGG.GGGC.CGGGCGGGGGAGGGGGCAGGGCGGTCCTCAGGCCGCGGTGGCGCCGCGGGCGCGGAGGATCGGCCAGGCCCGCTTGACCGACa                   <  2:3810833/101‑1 (MQ=255)                                                                                       cctcctTGCGGC.GGGC.CCGGCGGGGGAGGGGGCAGGGCGGTCCTCAGGCCGCGGTGGCGCCGCGGGCGCGGAGGATCGGCCAGGCCCGCTTGACCGACAgc                 <  2:1123798/101‑1 (MQ=255)                                                                                        ctcctTGCGGC.GGGC.CCGGCGGGGGAGGGGGCAGGGCGGTCCTCAGGCCGCGGTGGCGCCGCGGGCGCGGAGGATCGGCCAGGCCCGCTTGACCGACAgcg                <  2:3361999/101‑1 (MQ=255)                                                                                        ctcctTGCGGC.GGGC.CCGGCGGGGGAGGGGGCAGGGCGGTCCTCAGGCCGCGGTGGCGCCGCGGGCGCGGAGGATCGGCCAGGCCCGCTTGACCGACAgcg                <  2:2657207/101‑1 (MQ=255)                                                                                            ttGCGGC.GGGC.CCGGCGGGGGAGGGGGCAGGGCGGTCCTCAGGCCGCGGTGGCGCCGCGGGCGCGGAGGATCGGCCAGGCCCGCTTGACCGACAGCGCGAc            <  1:313096/101‑1 (MQ=255)                                                                                            tggcggg.gGGC.CCGGCGGGGGAGGGGGCAGGGCGGTCCTCAGGCCGCGGTGGCGCCGCGGGCGCGGAGGATCGGCCAGGCCCGCTTGACCGACAGCGCGAc            <  1:4431505/99‑1 (MQ=255)                                                                                             tGCGGC.GGGC.CCGGCGGGGGGGGGGGCAGGGCGGTCCTCAGGCCGCGGTGGCGCCGCGGGCGCGGAGGATCGGCCAGGCCCGCTTGACCGACAGCGCGAcc           <  2:3255395/101‑1 (MQ=255)                                                                                             tGCGGC.GGGC.CCGGCGGGGGAGGGGGCAGTGCGGTCCTCAGGCCGCGGTGGCGCCGCGGGCGCGGAGGATCGGCCAGGCCCGCTTGACCGACAGCGCGAcc           <  2:2883741/101‑1 (MQ=255)                                                                                              gcggc.gGGC.GCGCCGGGGGAGGGGGCAGGGCGGTCCTCGGGCCGCGGTGGCGCCGCGGCCGCGGAGGATCGGCCAGGCCCGCTTGACCGACAGCGCGACcg          <  2:2273300/101‑1 (MQ=255)                                                                                               gggc.ggGC.CCGGCGGGGGAGGGGGCAGGGCGGTCCTCAGGCCGCGGTGGCGCCGCGGGCGCGGAGGATCGGCCAGGCCCGCTTGACCGACAGCGCGACcgc         <  1:277917/100‑1 (MQ=255)                                                                                               cggc.ggGC.CCGGCGGGGGAGGGGGCAGGGCGGTCCTCAGGCCGCGGTGGCGCCGCGGGCGCGGAGGATCGGCCAGGCCCGCTTGACCGACAGCGCGACcgc         <  1:2199051/101‑1 (MQ=255)                                                                                                  c.ggGC.CCGGCGGGGGAGGGGGCAGGGCGGTCCTCAGGCCGCGGTGGCGCCGCGGGCGCGGAGGATCGGCCAGGCCCGCTTGACCGACAGCGCGACCccggc      >  1:4434715/1‑101 (MQ=255)                                                                                                    gggC.CCGGCGGGGGAGGGGGCAGGGCGGTCCTCAGGCCGCGGTGGCGCCGCGGGCGCGGAGGATCGGCCAGGCCCGCTTGACCGACAGCGCGACCgcggcg     >  2:3761812/1‑101 (MQ=255)                                                                                                    gggC.CCGGCGGGGGAGGGGGCAGGGCGGTCCTCAGGCCGCGGTGGCGCCGCGGGCGCGGAGGATCGGCCAGGCCCGCTTGACCGACAGCGCGACCgcggcg     >  1:1206389/1‑101 (MQ=255)                                                                                                       c.ccGGCGGGGGAGGGGGCAGGGCGGTCCTCAGGCCGCGGTGGCGCCGCGGGCGCGGAGGATCGGCCAGGCCCGCTTGACCGACAGCGCGACCGCGGCGGTg  <  1:1378972/101‑1 (MQ=255)                                                                                                       |                                                                                                      ATCGCCAGCAAAAGAAGCATGTAGGTGTCGATGCCGAAGCGTTTGAGCGGGTTCATCTTTCGTCCTTTCACGCAAGAGCC.GGGCCCCTCCTTGCGGC.GGGC.CCGGCGGGGGAGGGGGCAGGGCGGTCCTCAGGCCGCGGTGGCGCCGCGGGCGCGGAGGATCGGCCAGGCCCGCTTGACCGACAGCGCGACCGCGGCGGTG  >  NC_014035/102686‑102886 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG