Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,732,707 3 bp→TCC 100% intergenic (‑131/‑270) RCAP_rcc01601 ← / → tgt FeS assembly SUF system protein/queuine tRNA‑ribosyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,732,7070G→T100.0% 75.2 36intergenic (‑131/‑272)RCAP_rcc01601/tgtFeS assembly SUF system protein/queuine tRNA‑ribosyltransferase
Reads supporting (aligned to +/- strand):  new base (0/33):  ref base (1/1):  total (1/35)
*NC_0140341,732,7080G→C100.0% 85.5 36intergenic (‑132/‑271)RCAP_rcc01601/tgtFeS assembly SUF system protein/queuine tRNA‑ribosyltransferase
Reads supporting (aligned to +/- strand):  new base (0/34):  ref base (1/1):  total (1/35)
*NC_0140341,732,7090G→C100.0% 89.8 36intergenic (‑133/‑270)RCAP_rcc01601/tgtFeS assembly SUF system protein/queuine tRNA‑ribosyltransferase
Reads supporting (aligned to +/- strand):  new base (0/35):  ref base (1/0):  total (1/35)

GTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GA.CCGGGC..GGGCG..G..G..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATGCC  >  NC_014034/1732612‑1732780
                                                                                                   |||||||                                                                         
gTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGGCGCGGC.GG.CCCCGG..GGGGg..g..g..ggg                                                                      >  1:3211375/1‑99 (MQ=255)
  aTCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGCGGC.CCGGGC..GGGGg..g..g..gggc                                                                     >  1:3565799/1‑98 (MQ=255)
  aTCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GA.CC.CGC..GGCCg..g..g..gggacc                                                                   >  2:3482098/1‑96 (MQ=255)
   tCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GA.CCCGGG..GGGGg..g..g..gggccc                                                                   >  2:3258430/1‑96 (MQ=255)
    cGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GA.Cccggg..ggggg..g..gcgggcac                                                                    >  2:4404700/1‑94 (MQ=255)
     ggggggAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCCGGGG..GGGGG..G..G..Gcgcccc                                                                  >  1:2144327/1‑100 (MQ=255)
            gagGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GAGCCGGGC..CGGGG..G..G..GCGCCGGCCccccc                                                           >  2:3598854/1‑101 (MQ=255)
             agGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCCGGGC..GGGGG..G..G..GCGCCCCCCcccccc                                                          >  1:1317387/1‑100 (MQ=255)
              ggTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCCGGGCGGGGGCT..C..C..GCCCCCGCAccccc                                                           <  2:4112580/101‑1 (MQ=25)
               gTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCCGGGCGGGGGCT..C..C..GCCCCCGCACCCCCg                                                          <  2:4150718/101‑1 (MQ=25)
               gTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCCGGGCGGGGGCT..C..C..GCCCCCGCACCCCCg                                                          <  1:1866188/101‑1 (MQ=25)
                tAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCCGGGCGGGGGCT..C..C..GCCCCCGCACCCCCgg                                                         <  2:2123651/101‑1 (MQ=25)
                tAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCCGGGC..AGG.G..G..G..CCCCCCGCCccggctcccc                                                      >  2:2207937/1‑93 (MQ=255)
                 aaaGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCCGGGG..GGGGG..G..G..GCCCCCCCCcccccccccc                                                      >  2:2846371/1‑96 (MQ=255)
                 aaaGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCCGGGC..GGGGGATC..C..GCCCCCGCACCCCCGGc                                                        <  1:3049891/101‑1 (MQ=37)
                 aaaGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCCGGGC..GGGGG..G..G..CCGCCCCCCcccccccccc                                                      >  2:452225/1‑96 (MQ=255)
                  aaGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCCGGGCGGGGGCT..C..C..GCCCCCGCACCCCCGGCg                                                       <  1:2230478/101‑1 (MQ=25)
                   aGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCCGGGCGGGGGCT..C..C..GCCCCCGCACCCCCGGCGt                                                      <  2:2039214/101‑1 (MQ=25)
                    ggCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCCGGGCGGGGGCT..C..C..GCCCCCGCACCCCCGGCGTa                                                     <  1:4291833/101‑1 (MQ=25)
                     gCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCCGGGCGGGGGCT..C..C..GCCCCCGCACCCCCGGCGTAt                                                    <  1:2822342/101‑1 (MQ=25)
                       cGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCCGGGCGGGGGCT..C..C..GCCCCCGCACCCCCGGCGTAttt                                                  <  2:2197933/101‑1 (MQ=25)
                             tGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCCGGGCGGGGGCT..C..C..GCCCCCGCACCCCCGGCGTATTTGCGCCa                                            <  1:3599102/101‑1 (MQ=21)
                                ggggCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCCGGGCGGGGGCT..C..C..GCCCCCGCACCCCCGGCGTATTTGCGCCaaga                                         <  2:4033100/101‑1 (MQ=21)
                                 gggCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCCGGGCGGGGGCT..C..C..GCCCCCGCACCCCCGGCGTATTTGCGCCaagaa                                        <  2:937029/101‑1 (MQ=21)
                                  ggCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCCGGGCGGGGGCT..C..C..GCCCCCGCACCCCCGGCGTATTTGCGCCaagaag                                       <  2:1852620/101‑1 (MQ=18)
                                  ggCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCCGGGCGGGGGCT..C..C..GCCCCCGCACCCCCGGCGTATTTGCGCCaagaag                                       <  2:2109448/101‑1 (MQ=18)
                                    cGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCCGGGCGGGGGCT..C..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAgaagaa                                     <  1:2161466/101‑1 (MQ=18)
                                     ggAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCCGGGCGGGGGCT..C..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGaaa                                    <  2:1299150/101‑1 (MQ=18)
                                      gAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCCGGGCGGGGGCT..C..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAAc                                   <  2:2078712/101‑1 (MQ=18)
                                        aaGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCCGGGCGGGGGCT..C..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACaa                                 <  1:3469757/101‑1 (MQ=18)
                                        aaGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCCGGGCGGGGGCT..C..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACaa                                 <  1:3918651/101‑1 (MQ=18)
                                        aaGCCGCGTGACAAGCAGGGTTTCGCCGCGGCGGGGCAGCGGC.GACCCGGGCAGGGGCT..C..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACaa                                 <  2:1058920/101‑1 (MQ=18)
                                          gCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCCGGGCGGGGGCT..C..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGc                               <  2:463680/101‑1 (MQ=18)
                                          gCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCCGGGCGGGGGCT..C..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGc                               <  1:3525803/101‑1 (MQ=18)
                                               gTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCCGGGCGGGGGCT..C..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGAc                          <  2:3214114/101‑1 (MQ=18)
                                                tGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCCGGGCGGGGGCT..C..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGAcc                         <  1:2781204/101‑1 (MQ=18)
                                                 gCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GACCCGGGCGGGGGCT..C..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCa                        <  1:3881522/101‑1 (MQ=18)
                                                         gggTTTCGCCGCGGCGGGGCCGCGGC.GACCCGGGCGGGGGCT..C..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGcccccc                <  2:2977817/101‑1 (MQ=18)
                                                          ggTTTCGCCGCGGCGGGGCCGCGGC.GACCCGGGCGGGGGCT..C..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCt               <  2:3015952/101‑1 (MQ=18)
                                                          ggTTTCGCCGCGGCGGGGCCGCGGC.GACCCGGGCGGGGGCT..C..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCt               <  2:2144188/101‑1 (MQ=18)
                                                            tttCGCCGCGGCGGGGCCGCGGC.GACCCGGGCGGGGGCT..C..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGc             <  2:1831561/101‑1 (MQ=18)
                                                             ttCGCCGCGGCGGGGCCGCGGC.GACCCGGGC..GGGGT..GTCC..GCCGCCACGCCCCTGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCa            <  2:3696035/101‑1 (MQ=25)
                                                             ctccccgcTGCGGGGCCGCCGC.GACCCGGGCGGGGGCC..C..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCa            <  2:2006285/97‑1 (MQ=21)
                                                                gccgcGGCGGGGCCGCGGC.GACCCGGGCGGGGGCT..C..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGcc         <  2:667191/101‑1 (MQ=18)
                                                                   gcggggGGGCCGCGGC.GACCCGGGCGGGGGCT..C..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCa      <  2:1317290/101‑1 (MQ=18)
                                                                    cggcggGGCCGCGGC.GACCCGGGG..GGGGG..G..GCCGCCCCCCCCCCCCCCGCGGATTTTCGCCCAGAAGAAACAAACGGGGCCAAGCCCCCCCCCACCCGccg     >  2:2082861/1‑99 (MQ=255)
                                                                     ggcggGGCCGCGGC.GACCCGGGCGGGGGCT..C..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATg    <  1:3258662/101‑1 (MQ=18)
                                                                       cggGGCCGCGGC.GACCCGGGCGGGGGCT..C..C..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATGcc  <  1:298500/101‑1 (MQ=18)
                                                                                                   |||||||                                                                         
GTATCGGGGGGAGAGGTAAAGGCCGTCGGTGCGGGGCGGAAAGCCGCGTGCCAAGCAGGGTTTCGCCGCGGCGGGGCCGCGGC.GA.CCGGGC..GGGCG..G..G..GCCCCCGCACCCCCGGCGTATTTGCGCCAAGAAGAAACAAGCGGGACCAGGCCCCCCTGCAGCCGCATGCC  >  NC_014034/1732612‑1732780

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 38 ≤  ATCG