Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,733,287 2 bp→GC 100% coding (309‑310/1128 nt) tgt → queuine tRNA‑ribosyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,733,2870C→G100.0% 648.1 174S103S (TCC→TCGtgtqueuine tRNA‑ribosyltransferase
Reads supporting (aligned to +/- strand):  new base (92/82):  ref base (0/0):  total (92/82)
*NC_0140341,733,2880G→C100.0% 651.9 174M104M (GTG→CTG) tgtqueuine tRNA‑ribosyltransferase
Reads supporting (aligned to +/- strand):  new base (92/82):  ref base (0/0):  total (92/82)

Only 100 of 202 total aligned reads displayed.

GCATCGCGCGGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCCGTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGATCCAGAA  >  NC_014034/1733193‑1733382
                                                                                               ||                                                                                              
gcATCGCGCGGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTgcgc                                                                                           >  1:3337365/1‑101 (MQ=255)
    cgcgcgGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGc                                                                                       >  2:2260254/1‑101 (MQ=255)
     gcgcgGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCt                                                                                      <  1:2762587/101‑1 (MQ=255)
      cgcgGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTg                                                                                     <  1:4193863/101‑1 (MQ=255)
       gcgGCTGGGCGGGTTTCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGa                                                                                    <  1:1353145/101‑1 (MQ=255)
        gatcTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGAc                                                                                   <  1:3462259/98‑1 (MQ=255)
        cgGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGAc                                                                                   <  1:3122408/101‑1 (MQ=255)
         ggCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGAcc                                                                                  >  1:2605025/1‑101 (MQ=255)
           cTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCga                                                                                >  1:3774880/1‑101 (MQ=255)
             gggCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCgagg                                                                              >  2:1648392/1‑101 (MQ=255)
             gggCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCgagg                                                                              <  1:1076610/101‑1 (MQ=255)
               ggcggTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCgaggag                                                                            <  2:3608547/98‑1 (MQ=255)
                 gggTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAggg                                                                          >  2:3159060/1‑101 (MQ=255)
                  ggTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGc                                                                         >  1:1163009/1‑101 (MQ=255)
                  ggTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGc                                                                         >  2:69992/1‑101 (MQ=255)
                  ggTTGCACAAGTTCATGAACTGGGAGCGCCAGATCCTGACCGAATCGGCCGGG.TTTCAGGTGATGAGCCTTTGTTCGCTGCGCAAGCTGACCGAGGAGGGc                                                                         <  2:3682515/101‑1 (MQ=255)
                    ttGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCgg                                                                       >  2:581558/1‑100 (MQ=255)
                    ttGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGt                                                                       <  2:1229756/101‑1 (MQ=255)
                     tGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTg                                                                      <  1:4274734/101‑1 (MQ=255)
                     tGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTg                                                                      <  1:406597/101‑1 (MQ=255)
                     tGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTg                                                                      <  2:3268372/101‑1 (MQ=255)
                       cacaAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGAc                                                                    <  1:3620582/101‑1 (MQ=255)
                        acaAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGAcc                                                                   <  1:3385605/101‑1 (MQ=255)
                         caAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCt                                                                  >  1:2494105/1‑101 (MQ=255)
                          aaGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCtt                                                                 >  2:3413470/1‑101 (MQ=255)
                           aggtCATGAACTGGGACCGCCCGCTCCTGACCGACTCCGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCttt                                                                <  1:3104129/98‑1 (MQ=255)
                             ttCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTc                                                              >  1:4115821/1‑101 (MQ=255)
                                aTGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCACGCTGACCGAGGAGGGCGTGACCTTTTCCAg                                                           <  1:3971164/101‑1 (MQ=255)
                                aTGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAg                                                           >  1:1727385/1‑101 (MQ=255)
                                 tGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGc                                                          >  2:2866305/1‑101 (MQ=255)
                                 tGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGc                                                          <  1:46118/101‑1 (MQ=255)
                                   aaCTGGGAGCGCCCGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCa                                                        <  2:4017756/101‑1 (MQ=255)
                                    aCTGGGAGCGCCCGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGGCCGAGGAGGGCGTGACCTTTTCCAGCCat                                                       >  1:4023214/1‑101 (MQ=255)
                                      tGGGAGCGCCCGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCatat                                                     >  2:1951051/1‑101 (MQ=255)
                                        ggAGCGCCCGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCg                                                   <  2:2031421/101‑1 (MQ=255)
                                        ggAGCGCCCGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCATTGCTTCGCTGCGCAAGCTGACCGAGGAGGGAGTGACCTTTTTCAGCCATATCg                                                   >  2:4119216/1‑101 (MQ=255)
                                           gcgcCCGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGaag                                                <  2:1046705/101‑3 (MQ=255)
                                           gcgcCCGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACg                                                <  2:4152603/101‑1 (MQ=255)
                                           gcgcCCGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACg                                                >  2:1706777/1‑101 (MQ=255)
                                            cgcCCGATCCTGCCCGACTGGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAGGCTGACGGAGGAGGGCGTGACCTTTTCCAGCCATATCGACgg                                               <  2:2229226/101‑1 (MQ=255)
                                            cccccGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACgg                                               <  1:286777/99‑1 (MQ=255)
                                              cccGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCt                                             >  2:2702836/1‑101 (MQ=255)
                                                cGATCTTGACCGACTCGGGGGGG.TTTCAGTTGATGAGCCTTGCTTCGCTGCGCAGGCTGCCCGAGGAGGCCGTGCCCTTTCCCAGCCATATCGACGGCTCg                                           <  2:1612213/101‑1 (MQ=255)
                                                cGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCg                                           >  2:3902895/1‑101 (MQ=255)
                                                cGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCg                                           <  2:2894813/101‑1 (MQ=255)
                                                 gATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGa                                          >  1:744264/1‑101 (MQ=255)
                                                    ccTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAac                                       >  2:3814299/1‑101 (MQ=255)
                                                    ccTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAac                                       >  1:1652141/1‑101 (MQ=255)
                                                    ccTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAac                                       <  1:1794534/101‑1 (MQ=255)
                                                    ccTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAac                                       <  1:1696254/101‑1 (MQ=255)
                                                      tGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAacac                                     >  1:3022791/1‑101 (MQ=255)
                                                      tGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAacac                                     <  2:1773558/101‑1 (MQ=255)
                                                      tGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAacac                                     >  1:2025737/1‑101 (MQ=255)
                                                       ggccGCCTCGGGCGGG.TTTGAGGTGATGATCCTTGTTTCGCTGGGCAATCTGACCGGGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACAcc                                    <  1:1581283/99‑1 (MQ=255)
                                                         ccGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCAt                                  >  2:3167896/1‑101 (MQ=255)
                                                              tggggcggg.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTc                             <  1:128501/99‑1 (MQ=255)
                                                              tCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTc                             <  2:3544687/101‑1 (MQ=255)
                                                               tgggcgggtTTTCAGGTGAGAAGCCTTGCTCCGCCGCCAAAGCGGACCGAGGAGGGGCTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTc                             <  2:817157/100‑1 (MQ=255)
                                                               cgggcggg.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCg                            <  2:2493942/101‑1 (MQ=255)
                                                               cgggcggg.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCg                            <  2:2604854/101‑1 (MQ=255)
                                                                gggcggg.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGc                           >  2:1507676/1‑101 (MQ=255)
                                                                gggcggg.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGc                           >  2:4255159/1‑101 (MQ=255)
                                                                 ggcggg.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGcc                          >  2:1860425/1‑101 (MQ=255)
                                                                 ggcggg.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGcc                          >  1:1924604/1‑101 (MQ=255)
                                                                 ggcggg.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGcc                          >  2:2072869/1‑101 (MQ=255)
                                                                 ggcggg.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGcc                          <  2:3756899/101‑1 (MQ=255)
                                                                  gcggg.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGccc                         <  1:1834902/101‑1 (MQ=255)
                                                                  gcggg.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGccc                         <  1:2941869/101‑1 (MQ=255)
                                                                  gcggg.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGccc                         <  1:920387/101‑1 (MQ=255)
                                                                  gcggg.TGTCAGGGGAGGAGCCTTGCTTCGCTGCGAAAGCTGCCCGAGGAGGCCGTGCCCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGccc                         <  2:292210/101‑1 (MQ=255)
                                                                    ggg.TTTCAGGTGATGGGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGa                       >  1:3751205/1‑101 (MQ=255)
                                                                    ggg.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGa                       >  1:1223062/1‑101 (MQ=255)
                                                                    ggg.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGa                       >  1:2246193/1‑101 (MQ=255)
                                                                      gatttCAGGTGATGAGCCTTGCTTCGCTGCGTAAGCTTACCGAGGAGGGCGTGACTTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGaa                      <  2:3327360/99‑1 (MQ=255)
                                                                      g.TTTCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAAc                     >  1:1461236/1‑101 (MQ=255)
                                                                      g.TTTCAGGTGAAGAGCCTCCCTTCGCTGCGCAAGCTGACCGAGGAGGGTGTCCCCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAAc                     <  1:1618378/101‑1 (MQ=255)
                                                                        tttCAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACg                    <  2:1162918/101‑1 (MQ=255)
                                                                           cAGGTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTc                 >  1:976950/1‑101 (MQ=255)
                                                                           cAGGAGATGAGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACAGCTCGAAACACCAACTGTAGCCCGAACgacc                 >  2:3950745/1‑98 (MQ=255)
                                                                              gTGATGAGCCTTGCTTCGCTGCGCAAGCTGACCTAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGAt              <  1:3814552/101‑1 (MQ=255)
                                                                               tgatggGCCTTGCTTCGCGGCGCACGATGAGCGGGAGGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATg             <  2:3291800/101‑1 (MQ=255)
                                                                               tgatgaGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATg             >  1:3669999/1‑101 (MQ=255)
                                                                               taatgaGCCTTGCTTCCCTGCGCAGGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATg             <  1:3526918/99‑1 (MQ=255)
                                                                                gatgaGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATgg            <  2:3337128/101‑1 (MQ=255)
                                                                                gatgaGCCTTGCTTCGCGGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATgg            <  1:70005/101‑1 (MQ=255)
                                                                                   gaGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGgaga         >  2:4401791/1‑101 (MQ=255)
                                                                                   gaGCCTTGCTCCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGgaga         <  1:2703026/101‑1 (MQ=255)
                                                                                    aGCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGAt        <  2:1838416/101‑1 (MQ=255)
                                                                                     gCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGATc       >  2:4275119/1‑101 (MQ=255)
                                                                                     gCCTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGATc       >  1:1398240/1‑101 (MQ=255)
                                                                                       cTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGATCCa     >  2:449128/1‑101 (MQ=255)
                                                                                       cTTGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGATCCa     >  2:1278625/1‑101 (MQ=255)
                                                                                        ttGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGATcccg    >  2:1243662/1‑99 (MQ=255)
                                                                                        ttGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGATCCAg    >  1:2658664/1‑101 (MQ=255)
                                                                                        ttGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGATCCAg    >  2:3213989/1‑101 (MQ=255)
                                                                                         tGCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGATCCAGa   >  2:3982111/1‑101 (MQ=255)
                                                                                          gCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGATCCAGaa  <  2:133249/101‑1 (MQ=255)
                                                                                          gCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGATCCAGaa  >  2:2469575/1‑101 (MQ=255)
                                                                                          gCTTCGCTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGATCCAGaa  >  1:3201034/1‑101 (MQ=255)
                                                                                          gCTTCGCTGCGAAAGCTGACCGGGGAGGGCGGGCCCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGATCCAGaa  <  1:590098/101‑1 (MQ=255)
                                                                                               ||                                                                                              
GCATCGCGCGGCTGGGCGGGTTGCACAAGTTCATGAACTGGGAGCGCCCGATCCTGACCGACTCGGGCGGG.TTTCAGGTGATGAGCCTTGCTTCCGTGCGCAAGCTGACCGAGGAGGGCGTGACCTTTTCCAGCCATATCGACGGCTCGAAACACCATCTGTCGCCCGAACGCTCGATGGAGATCCAGAA  >  NC_014034/1733193‑1733382

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG