Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,802,277 2 bp→GC 100% coding (660‑661/2124 nt) ligA → DNA ligase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,802,2770C→G100.0% 658.0 179S220S (TCC→TCGligADNA ligase
Reads supporting (aligned to +/- strand):  new base (97/82):  ref base (0/0):  total (97/82)
*NC_0140341,802,2780G→C100.0% 657.6 178M221M (GTG→CTG) ligADNA ligase
Reads supporting (aligned to +/- strand):  new base (96/81):  ref base (0/1):  total (96/82)

Only 100 of 186 total aligned reads displayed.

TACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGACGTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCCGTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGCTCTCGGACACGC  >  NC_014034/1802182‑1802371
                                                                                               ||                                                                                             
tACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTgcg                                                                                           <  1:4324260/101‑1 (MQ=255)
tACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTgcg                                                                                           <  2:946705/101‑1 (MQ=255)
tACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTgcg                                                                                           <  1:947126/101‑1 (MQ=255)
tACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTgcg                                                                                           <  1:2228293/101‑1 (MQ=255)
 aCATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTgcgc                                                                                          >  1:3035556/1‑101 (MQ=255)
  cATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGcc                                                                                         >  2:3261350/1‑101 (MQ=255)
  cATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGcc                                                                                         >  1:3268143/1‑101 (MQ=255)
  cATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGcc                                                                                         >  1:1107210/1‑101 (MQ=255)
  cATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGcc                                                                                         >  1:2806497/1‑101 (MQ=255)
   aTGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCa                                                                                        <  2:1243177/101‑1 (MQ=255)
    tGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAg                                                                                       >  2:3545968/1‑101 (MQ=255)
    tGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAg                                                                                       >  2:4039500/1‑101 (MQ=255)
     gAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGc                                                                                      >  1:1733723/1‑101 (MQ=255)
      aGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCt                                                                                     <  2:987502/101‑1 (MQ=255)
       gCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTc                                                                                    >  1:545125/1‑101 (MQ=255)
       gCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTc                                                                                    >  1:1168533/1‑101 (MQ=255)
       gCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTc                                                                                    <  2:3147653/101‑1 (MQ=255)
          aTGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGAc                                                                                 <  2:3441699/101‑1 (MQ=255)
           tGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACg                                                                                <  2:3986980/101‑1 (MQ=255)
            gCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGc                                                                               <  2:1570542/101‑1 (MQ=255)
            gCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGc                                                                               >  2:3475712/1‑101 (MQ=255)
            gCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGc                                                                               >  1:4412087/1‑101 (MQ=255)
              cGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATTCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGccc                                                                             <  2:2277189/101‑1 (MQ=255)
               gATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCg                                                                            >  1:2326984/1‑101 (MQ=255)
                 ttttGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGa                                                                          >  2:2760672/1‑101 (MQ=255)
                 ttttGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGa                                                                          >  1:65202/1‑101 (MQ=255)
                    tGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCa                                                                       <  1:1210281/101‑1 (MQ=255)
                    tGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCa                                                                       >  2:4293802/1‑101 (MQ=255)
                    tGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCa                                                                       <  2:2801526/101‑1 (MQ=255)
                      cggcgGTGAATGCGCGACAGTGGGCGCGGGGCGACAAGAGCTTAGCGAATCCGCGAAATGCCGCCGCGGGCTCGCTGGGCCAGCTGGACGCCCTGATCGcc                                                                     <  2:4448643/101‑1 (MQ=255)
                       ggcgCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCg                                                                    >  2:1519707/1‑101 (MQ=255)
                       ggcgCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCg                                                                    >  2:2015466/1‑101 (MQ=255)
                          gcTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCt                                                                 <  1:3592685/101‑1 (MQ=255)
                           cTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTc                                                                >  2:2308283/1‑101 (MQ=255)
                            tGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTcc                                                               >  1:1029899/1‑101 (MQ=255)
                            tGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTcc                                                               <  2:4047552/101‑1 (MQ=255)
                                tGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGAc                                                           >  1:864567/1‑101 (MQ=255)
                                 gcgcGGCAGGCGGAGCGGGGCTACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGCCGCCCGGATCACCGCCTCCAGAcc                                                          <  2:736626/101‑1 (MQ=255)
                                  cgcgGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGAccc                                                         >  1:163596/1‑101 (MQ=255)
                                   gcgGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCt                                                        <  2:2030968/101‑1 (MQ=255)
                                   gcgGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCt                                                        <  1:3281891/101‑1 (MQ=255)
                                     ggCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGGCGCCCGGATCACCGCCTCCAGGCCCTTg                                                      >  1:3400627/1‑101 (MQ=255)
                                     ggCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTg                                                      >  1:797355/1‑101 (MQ=255)
                                      gCAGGCGGCGCGGGGCGACAAGAGCTTCGCCAATCCGCGGAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGAGGCCCGGATCACCCGCTCCAGACCCttca                                                     >  2:2688184/1‑99 (MQ=255)
                                      gCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGa                                                     >  2:1374234/1‑101 (MQ=255)
                                       cAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGaa                                                    >  2:2125408/1‑101 (MQ=255)
                                        aGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGaaa                                                   >  2:290703/1‑101 (MQ=255)
                                        aGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGaaa                                                   >  1:1992188/1‑101 (MQ=255)
                                          gCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAAtt                                                 <  1:1305753/101‑1 (MQ=255)
                                           cGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTc                                                <  1:95370/101‑1 (MQ=255)
                                            ggggCGGGGCCACAAGAGCGTCCCCAATCCGCGCAAGGCCGGCGCGGGCCCGCTGCGCCAGCTCGACGCCCGGGTCACCGCCTCCAGACCCTTGAAATTCt                                               <  2:3439176/98‑1 (MQ=255)
                                            ggAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAACTTCt                                               >  2:703873/1‑101 (MQ=255)
                                            ggAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCt                                               >  1:588840/1‑101 (MQ=255)
                                              aGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCttt                                             <  2:3055294/101‑1 (MQ=255)
                                              aGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCttt                                             <  2:3302935/101‑1 (MQ=255)
                                               gCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTg                                            >  1:3997794/1‑101 (MQ=255)
                                                cGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGc                                           <  2:3062161/101‑1 (MQ=255)
                                                cGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGc                                           >  2:960082/1‑101 (MQ=255)
                                                cGGGGCGACAAGACCTTCGCCAATCCGCGCAATTACGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGc                                           <  1:3828019/101‑1 (MQ=255)
                                                  gggCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCt                                         >  1:2822641/1‑101 (MQ=255)
                                                   ggCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTa                                        >  1:1051210/1‑101 (MQ=255)
                                                   ggCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTa                                        <  1:748098/101‑1 (MQ=255)
                                                   ggCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTa                                        <  2:1271289/101‑1 (MQ=255)
                                                    gCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTAt                                       <  2:65189/101‑1 (MQ=255)
                                                    gCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTAt                                       <  2:2517890/101‑1 (MQ=255)
                                                     cGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTAtt                                      <  1:3544245/101‑1 (MQ=255)
                                                      gACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTc                                     <  2:1694577/101‑1 (MQ=255)
                                                      gACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTc                                     <  1:2476467/101‑1 (MQ=255)
                                                       aCAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTcc                                    >  1:3231397/1‑101 (MQ=255)
                                                        cAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCt                                   <  2:3666275/101‑1 (MQ=255)
                                                        cAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCt                                   >  2:4136693/1‑101 (MQ=255)
                                                         aagagcttCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTg                                  >  1:2440481/7‑101 (MQ=255)
                                                         aagagcttCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTg                                  <  2:3034054/95‑1 (MQ=255)
                                                         aagagcttCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTg                                  >  1:2718902/7‑101 (MQ=255)
                                                          atagcttCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTgg                                 >  2:56328/6‑101 (MQ=255)
                                                          agagcttCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTgg                                 >  1:1610400/6‑101 (MQ=255)
                                                          agagcttCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCAGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTgg                                 <  1:696932/96‑1 (MQ=255)
                                                             gcttCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGc                              >  1:180267/3‑101 (MQ=255)
                                                               ttCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGt                            >  2:3781569/1‑101 (MQ=255)
                                                               ttCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGt                            >  2:3004754/1‑101 (MQ=255)
                                                                 cGCCAATCCGCGTAATGCCGCCGTGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTc                          <  2:3982999/101‑1 (MQ=255)
                                                                      aTCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTcc                     <  1:1001853/101‑1 (MQ=255)
                                                                       tCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCg                    >  1:4412752/1‑101 (MQ=255)
                                                                        ccGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGa                   >  2:3595852/1‑101 (MQ=255)
                                                                        ccGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGa                   >  2:1171814/1‑101 (MQ=255)
                                                                        ccGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGa                   >  1:970375/1‑101 (MQ=255)
                                                                           cgcAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAAcc                >  2:1640852/1‑101 (MQ=255)
                                                                           cgcAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAAcc                >  1:2329340/1‑101 (MQ=255)
                                                                           cgcAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAAcc                <  2:1107122/101‑1 (MQ=255)
                                                                           cgcAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAAcc                >  1:4049617/1‑101 (MQ=255)
                                                                           cgcAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACACTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAAcc                >  2:1764551/1‑101 (MQ=255)
                                                                                tGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGctct           >  2:1815455/1‑101 (MQ=255)
                                                                                 gccgccGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGCtctc          >  1:3572371/1‑101 (MQ=255)
                                                                                  ccgccgCGGGCTCGGTGCGCCAGCTCGACGCCCCCATCGCCGCCTCCAGACCCTTGAAAGTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGCCCTCg         <  2:2175790/101‑1 (MQ=255)
                                                                                       gcgGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGCTCTCGGacac    <  1:3925260/101‑1 (MQ=255)
                                                                                        cgGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGCTCTCGGACACg   >  1:3148363/1‑101 (MQ=255)
                                                                                        cgGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGCTCTCGGACACg   <  2:1331819/101‑1 (MQ=255)
                                                                                        cgGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGCTCTCGGACACg   >  2:585274/1‑101 (MQ=255)
                                                                                         gggCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGCTCTCGGACACGc  <  1:2129462/101‑1 (MQ=255)
                                                                                         gggCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGCTCTCGGACACGc  >  2:1905080/1‑101 (MQ=255)
                                                                                               ||                                                                                             
TACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGACGTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCCGTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCTGGGGCGTTCTGTCCGAACCGCTCTCGGACACGC  >  NC_014034/1802182‑1802371

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG