Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,927,518 2 bp→TG 100% coding (185‑186/336 nt) yajC → preprotein translocase subunit YajC

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,927,5180G→T100.0% 818.0 219G62V (GGT→GTT) yajCpreprotein translocase subunit YajC
Reads supporting (aligned to +/- strand):  new base (120/99):  ref base (0/0):  total (120/99)
*NC_0140341,927,5190T→G100.0% 841.8 219G62G (GGT→GGGyajCpreprotein translocase subunit YajC
Reads supporting (aligned to +/- strand):  new base (120/99):  ref base (0/0):  total (120/99)

Only 100 of 225 total aligned reads displayed.

CTGATCTTCGTGATCATGTATTTCCTGCTGATCCGCCCGCAGCAAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGGTCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGAAATCGAGGTCGAGATCGCCAAGGACATGAAGGTGCGGATGA  >  NC_014034/1927418‑1927613
                                                                                                    ||                                                                                              
ctgatcTTCGTGATCATGTATTTCCTGCTGATCCGCCCGCAGCAAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATggg                                                                                                 >  2:2072178/1‑101 (MQ=255)
     cTTCGTGATCATGTATTTCCTGCTGATCCGCCCGCAGCAAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGa                                                                                            >  1:3135431/1‑101 (MQ=255)
     cTTCGTGATCATGTATTTCCTGCTGATCCGCCCGCAGCAAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGa                                                                                            >  2:1515721/1‑101 (MQ=255)
     cTTCGTGATCATGTATTTCCTGCTGATCCGCCCGCAGCAAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGa                                                                                            >  1:4442315/1‑101 (MQ=255)
       tCGTGATCATGTATTTCCTGCTGATCCGCCCGCAGCAAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACg                                                                                          <  2:1185117/101‑1 (MQ=255)
         gTGATCATGTATTTCCTGCTGATCCGCCCGCAGCAAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCa                                                                                        >  2:9160/1‑101 (MQ=255)
           gATCATGTATTTCCTGCTGATCCGCCCGCAGCAAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAgg                                                                                      >  2:1617485/1‑101 (MQ=255)
           gATCATGTATTTCCTGCTGATCCGCCCGCAGCAAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAgg                                                                                      <  1:665629/101‑1 (MQ=255)
            aTCATGTATTTCCTGCTGATCCGCCCGCAGCAAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGc                                                                                     >  1:1757426/1‑101 (MQ=255)
             tCATGTATTTCCTGCTGATCCGCCCGCAGCAAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCg                                                                                    >  1:3194343/1‑101 (MQ=255)
               aTGTATTTCCTGCTGATCCGCCCGCAGCAAAAAAAGGCCAAGGAACACAAGGCGATGGCCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCggg                                                                                  >  1:4092453/1‑101 (MQ=255)
               aTGCATTTCATGCTGATCCGCCCGCAGCAAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACAGGCTGGTGACGCAGGCggg                                                                                  >  2:1458009/1‑101 (MQ=255)
                tGTATTTCCTGCTGATCCGCCCGCAGCAAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGc                                                                                 <  2:3734334/101‑1 (MQ=255)
                tGTATTTCCTGCTGATCCGCCCGCAGCAAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGc                                                                                 <  1:4417381/101‑1 (MQ=255)
                tGTATTTCCTGCTGATCCGCCCGCAGCAAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGc                                                                                 >  1:1677171/1‑101 (MQ=255)
                tGTATTTCCTGCTGATCCGCCCGCAGCAAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGc                                                                                 <  1:4149676/101‑1 (MQ=255)
                tGTATTTCCTGCTGATCCGCCCGCAGCAAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGc                                                                                 <  1:2775784/101‑1 (MQ=255)
                  tATTTCCTGCTGATCCGCCCGCAGCAAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGcat                                                                               <  1:1272484/101‑1 (MQ=255)
                      tCCTGCTGATCCGCCCGCAGCAAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCatcatc                                                                           <  1:2980877/101‑1 (MQ=255)
                      tCCTGCTGATCCGCCCGCAGCAAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCatcatc                                                                           >  2:2764148/1‑101 (MQ=255)
                       ccTGCTGATCCGCCCGCAGCAAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCg                                                                          <  1:3537113/101‑1 (MQ=255)
                       ccTGCTGATCCGCCCGCAGCAAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCg                                                                          <  1:18944/101‑1 (MQ=255)
                       ccTGCTGATCCGCCCGCAGCAAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCg                                                                          <  2:4203011/101‑1 (MQ=255)
                        ctgctgATCCGCCCGCAGCAAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCgg                                                                         >  2:2513474/1‑101 (MQ=255)
                         tgctgATCCGCCCGCAGCAAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGc                                                                        >  1:2020871/1‑101 (MQ=255)
                         tgctgATCCGCCCGCAGCAAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGc                                                                        >  1:3705830/1‑101 (MQ=255)
                            tgATCCGCCCGCAGCAAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAg                                                                     >  1:2930585/1‑101 (MQ=255)
                               tCCGCCCGCAGCAAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTc                                                                  >  1:524516/1‑101 (MQ=255)
                                ccgcccgcGGCAAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCg                                                                 >  1:1173074/1‑101 (MQ=255)
                                ccgcccgcAGCAAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCg                                                                 >  2:1347563/1‑101 (MQ=255)
                                  gcccgcAGCAAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTg                                                               <  2:2020027/101‑1 (MQ=255)
                                  gcccgcAGCAAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTg                                                               >  2:1307385/1‑101 (MQ=255)
                                  gcccgcAGCAAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTg                                                               <  1:1515826/101‑1 (MQ=255)
                                   cccgcAGCAAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGc                                                              <  2:347376/101‑1 (MQ=255)
                                    ccgcAGCAAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCa                                                             <  2:1982242/101‑1 (MQ=255)
                                     cgcAGCAAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCAt                                                            <  1:2455064/101‑1 (MQ=255)
                                      gcagcaAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATc                                                           >  2:1921844/1‑101 (MQ=255)
                                       cagcaAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCt                                                          <  2:1760649/101‑1 (MQ=255)
                                       cagcaAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCt                                                          <  2:438389/101‑1 (MQ=255)
                                       cagcaAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGGCGTGCATCt                                                          >  1:206103/1‑101 (MQ=255)
                                        agcaAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTg                                                         >  2:2830831/1‑101 (MQ=255)
                                        agcaAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTg                                                         <  1:4261786/101‑1 (MQ=255)
                                         gcaAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTgc                                                        <  1:3056440/101‑1 (MQ=255)
                                         gcaAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTgc                                                        <  1:1972750/101‑1 (MQ=255)
                                          caAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGGCGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTgcg                                                       >  1:3755346/1‑101 (MQ=255)
                                            aaaaaaGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGcgcg                                                     <  2:1423123/101‑1 (MQ=255)
                                            aaaaaaGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGcgcg                                                     >  2:3419737/1‑101 (MQ=255)
                                             aaaaaGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGa                                                    >  1:1625349/1‑101 (MQ=255)
                                              aaaaGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAg                                                   >  1:632387/1‑101 (MQ=255)
                                               aaaGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAgg                                                  >  1:3304841/1‑101 (MQ=255)
                                               aaaGGCCAAGGAACACAAGGCAATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAgg                                                  >  1:1057779/1‑101 (MQ=255)
                                                aaGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGa                                                 >  1:2163136/1‑101 (MQ=255)
                                                aaGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGa                                                 <  2:443953/101‑1 (MQ=255)
                                                 aGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGAc                                                >  1:1412896/1‑101 (MQ=255)
                                                   gCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACgg                                              >  2:1744309/1‑101 (MQ=255)
                                                    ccAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGc                                             <  2:3967347/101‑1 (MQ=255)
                                                    ccAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGc                                             <  2:4178570/101‑1 (MQ=255)
                                                    ccAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGc                                             >  2:2972251/1‑101 (MQ=255)
                                                     cAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCg                                            <  1:2103177/101‑1 (MQ=255)
                                                     cAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCg                                            >  1:2654902/1‑101 (MQ=255)
                                                      aaGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGa                                           >  1:1335494/1‑101 (MQ=255)
                                                       aGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGaa                                          <  1:4042920/101‑1 (MQ=255)
                                                         gAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGAAAt                                        >  1:3468348/1‑101 (MQ=255)
                                                          aaCACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGAAATc                                       >  2:354547/1‑101 (MQ=255)
                                                          aaCACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGAAATc                                       >  2:3755101/1‑101 (MQ=255)
                                                            cacaAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGAAATCGa                                     <  2:4265820/101‑1 (MQ=255)
                                                            cacaAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGGGCTGACGCAGGCGGGCATCATCGGCAAGGACGTGCATATGCGCCAGGAAGGCGAAAACGa                                     >  2:2211824/1‑101 (MQ=255)
                                                             acaAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGAAATCGAg                                    <  1:1433152/101‑1 (MQ=255)
                                                             acaAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGAAATCGAg                                    <  2:3661944/101‑1 (MQ=255)
                                                               aaGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGAAATCGAGGt                                  <  1:3603623/101‑1 (MQ=255)
                                                                aGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGAAATCGAGGTc                                 <  1:2664535/101‑1 (MQ=255)
                                                                 ggCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGAAATCGAGGTCg                                >  2:579960/1‑101 (MQ=255)
                                                                  gCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGAAATCGAGGTCga                               <  1:2843052/101‑1 (MQ=255)
                                                                  gCGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGAAATCGAGGTCga                               >  2:1391363/1‑101 (MQ=255)
                                                                   cGATGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGAAATCGAGGTCgag                              <  2:1172983/101‑1 (MQ=255)
                                                                     aTGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGAAATCGAGGTCGAGAt                            >  1:1101807/1‑101 (MQ=255)
                                                                     aTGGTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGAAATCGAGGTCGAGAt                            >  2:2356365/1‑101 (MQ=255)
                                                                      ttgTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGAAATCGAGGTCGAGATc                           <  2:785414/99‑1 (MQ=255)
                                                                       ggTTGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGAAATCGAGGTCGAGATCg                          <  2:1386000/101‑1 (MQ=255)
                                                                       ggTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGAAATCGAGGTCGAGATCg                          >  2:1088053/1‑101 (MQ=255)
                                                                       ggTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGAAATCGAGGTCGAGATCg                          <  2:4092193/101‑1 (MQ=255)
                                                                        gTCGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGAAATCGAGGTCGAGATCGc                         >  2:3010231/1‑101 (MQ=255)
                                                                          cGATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGAAATCGAGGTCGAGATCGCCa                       >  1:2517232/1‑101 (MQ=255)
                                                                           gATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGAAATCGAGGTCGAGATCGCCaa                      >  1:3912842/1‑101 (MQ=255)
                                                                           gATGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGAAATCGAGGTCGAGATCGCCaa                      >  1:3473359/1‑101 (MQ=255)
                                                                            aTGCGCTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGAAATCGAGGTCGAGATCGCCAAg                     <  1:1179147/101‑1 (MQ=255)
                                                                               cgcTGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGAAATCGAGGTCGAGATCGCCAAGGAc                  <  1:3230769/101‑1 (MQ=255)
                                                                                  tGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGAAATCGAGGTCGAGATCGCCAAGGACATg               <  2:3552877/101‑1 (MQ=255)
                                                                                  tGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGAAATCGAGGTCGAGATCGCCAAGGACATg               >  1:2881822/1‑101 (MQ=255)
                                                                                  tGCGGCGTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGAAATCGAGGTCGAGATCGCCAAGGACATg               >  2:1006507/1‑101 (MQ=255)
                                                                                   gcggcgTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGAAATCGAGGTCGAGATCGCCAAGGACATGa              <  1:4401628/101‑1 (MQ=255)
                                                                                     ggcgTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGAAATCGAGGTCGAGATCGCCAAGGACATGAAg            >  2:2056614/1‑101 (MQ=255)
                                                                                      gcgTGGTGACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCCGCGCGAGGACGGCGAAATCGAGGTCGAGATCGCCAAGGACATGAAgg           <  2:3839217/101‑1 (MQ=255)
                                                                                        gtggtgACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGAAATCGAGGTCGAGATCGCCAAGGACATGAAGGTg         >  2:332337/1‑101 (MQ=255)
                                                                                        gtggtgACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGAAATCGAGGTCGAGATCGCCAAGGACATGAAGGTg         <  1:2737405/101‑1 (MQ=255)
                                                                                         tggtgACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGAAATCGAGGTCGAGATCGCCAAGGACATGAAGGTGc        >  2:3122640/1‑101 (MQ=255)
                                                                                           gtgACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGAAATCGAGGTCGAGATCGCCAAGGACATGAAGGTGCgg      >  2:782410/1‑101 (MQ=255)
                                                                                            tgACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGAAATCGAGGTCGAGATCGCCAAGGACATGAAGGTGCGga     <  2:939417/101‑1 (MQ=255)
                                                                                             gACATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGAAATCGAGGTCGAGATCGCCAAGGACATGAAGGTGCGgat    <  1:737410/101‑1 (MQ=255)
                                                                                               cATGGTGCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGAAATCGAGGTCGAGATCGCCAAGGACATGAAGGTGCGgatga  <  1:3275407/101‑1 (MQ=255)
                                                                                                    ||                                                                                              
CTGATCTTCGTGATCATGTATTTCCTGCTGATCCGCCCGCAGCAAAAAAAGGCCAAGGAACACAAGGCGATGGTCGATGCGCTGCGGCGTGGTGACATGGGTCTGACGCAGGCGGGCATCATCGGCAAGGTCGTGCATCTGCGCGAGGACGGCGAAATCGAGGTCGAGATCGCCAAGGACATGAAGGTGCGGATGA  >  NC_014034/1927418‑1927613

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG