Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,180,185 2 bp→GC 100% coding (874‑875/1314 nt) RCAP_rcc02022 → polyols ABC transporter periplasmic polyols‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,180,1850C→G100.0% 675.4 180R292G (CGC→GGC) RCAP_rcc02022polyols ABC transporter periplasmic polyols‑binding protein
Reads supporting (aligned to +/- strand):  new base (84/95):  ref base (0/0):  total (84/96)
*NC_0140342,180,1860G→C100.0% 680.0 180R292P (CGC→CCC) RCAP_rcc02022polyols ABC transporter periplasmic polyols‑binding protein
Reads supporting (aligned to +/- strand):  new base (84/96):  ref base (0/0):  total (84/96)

Only 100 of 182 total aligned reads displayed.

ATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCG.GCTGGC.CCGAACAAGG.C.GGTGTCCGCAAGCATGGCAACTGGCTCTGGGCCTGGAACCCTCGCCAATTCCGGCGGGCACGCAAAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGAC  >  NC_014034/2180093‑2180282
                                                                                                ||                                                                                                
tctcGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAg                                                                                              <  1:1906232/98‑1 (MQ=255)
 tGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGc                                                                                             <  1:4321407/101‑1 (MQ=255)
  gCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCa                                                                                            <  2:2571614/101‑1 (MQ=255)
  gCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCa                                                                                            >  2:201526/1‑101 (MQ=255)
  gCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCa                                                                                            >  1:563284/1‑101 (MQ=255)
       cGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCa                                                                                       >  2:2665036/1‑101 (MQ=255)
        gTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCaa                                                                                      <  2:1938655/101‑1 (MQ=255)
         tGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGTCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAAc                                                                                     <  1:1253029/101‑1 (MQ=255)
         tGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAAc                                                                                     <  1:874431/101‑1 (MQ=255)
          ggCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACt                                                                                    <  2:815622/101‑1 (MQ=255)
           gccgccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTg                                                                                   <  2:2117984/101‑1 (MQ=255)
             cgccTCTTTCGTGACCANCCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGc                                                                                 <  2:2215256/101‑1 (MQ=255)
              gccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGct                                                                                <  2:1443565/101‑1 (MQ=255)
              gccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGct                                                                                <  1:2671821/101‑1 (MQ=255)
               ccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGctc                                                                               <  1:3148623/101‑1 (MQ=255)
               ccTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGctc                                                                               <  2:4178223/101‑1 (MQ=255)
                ctctTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGctct                                                                              <  1:1177756/101‑1 (MQ=255)
                  ctTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTgg                                                                            <  1:943692/101‑1 (MQ=255)
                  ctTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTgg                                                                            <  1:2984355/101‑1 (MQ=255)
                   tttCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTggg                                                                           <  1:4116227/101‑1 (MQ=255)
                      cGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCt                                                                        >  2:3551383/1‑101 (MQ=255)
                      cGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCt                                                                        >  1:3049261/1‑101 (MQ=255)
                       gTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTg                                                                       <  2:3953616/101‑1 (MQ=255)
                        tGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTgg                                                                      >  2:3264058/1‑101 (MQ=255)
                         gACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGGGTCGCCAAGCATGGCGACTGGCTCTGGGCCTGGa                                                                     >  2:3813288/1‑101 (MQ=255)
                          aCCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGaa                                                                    <  1:3109403/101‑1 (MQ=255)
                          aCCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGaa                                                                    >  2:1771773/1‑101 (MQ=255)
                           ccAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGGAc                                                                   <  1:2558542/101‑1 (MQ=255)
                           ccAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAc                                                                   <  1:4028758/101‑1 (MQ=255)
                            cAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcc                                                                  >  1:3192805/1‑101 (MQ=255)
                            cAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcc                                                                  >  2:392816/1‑101 (MQ=255)
                            cAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTATGGGCCTGGAAcc                                                                  <  2:322219/101‑1 (MQ=255)
                             aaCCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcct                                                                 <  1:3389921/101‑2 (MQ=255)
                             aaCCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcct                                                                 >  1:3449772/1‑100 (MQ=255)
                             aaCCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcct                                                                 >  1:618763/1‑100 (MQ=255)
                              aCCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGG.CTGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctc                                                                >  2:2445230/1‑99 (MQ=255)
                               ccccAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctcg                                                               <  1:3551631/101‑4 (MQ=255)
                                cccAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctcgc                                                              >  2:4265824/1‑97 (MQ=255)
                                 ccAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGcc                                                            >  1:2724700/1‑101 (MQ=255)
                                 ccAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGcc                                                            >  2:4207801/1‑101 (MQ=255)
                                  cAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGTCGAACAAGGCC.GGTGTCGCGAAACATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCa                                                           >  1:3807045/1‑101 (MQ=255)
                                  cAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCa                                                           >  2:2505740/1‑101 (MQ=255)
                                   aaGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCat                                                          >  1:2511382/1‑100 (MQ=255)
                                    aGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCatt                                                         <  2:3624336/101‑3 (MQ=255)
                                    aGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCatt                                                         <  2:3084913/101‑3 (MQ=255)
                                     ggACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattc                                                        >  2:4345404/1‑98 (MQ=255)
                                     ggACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattc                                                        <  2:1262317/101‑4 (MQ=255)
                                      gACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattcc                                                       >  2:2969527/1‑97 (MQ=255)
                                       aCTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcg                                                     <  2:3449532/101‑1 (MQ=255)
                                       aCTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcg                                                     <  1:4334058/101‑1 (MQ=255)
                                        cTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcgg                                                    >  1:3879181/1‑101 (MQ=255)
                                        cTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcgg                                                    <  2:3589537/101‑1 (MQ=255)
                                         tCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACGAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcggc                                                   >  2:2270847/1‑101 (MQ=255)
                                         tCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcggc                                                   >  1:682200/1‑101 (MQ=255)
                                         tCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcggc                                                   <  2:2163111/101‑1 (MQ=255)
                                          cGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcggcg                                                  <  2:1597233/101‑1 (MQ=255)
                                           ggCCGTGGCCGATCATGTCTGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcggcgg                                                 >  1:4031035/1‑101 (MQ=255)
                                             ccGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGc                                               >  1:1552591/1‑101 (MQ=255)
                                               gTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGccc                                             >  1:2469111/1‑99 (MQ=255)
                                               gTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCAc                                             >  2:3502424/1‑101 (MQ=255)
                                                 ggCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGc                                           >  1:3029453/1‑101 (MQ=255)
                                                  gCCGATCATGTCGTCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCTCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCa                                          <  2:1391065/101‑1 (MQ=255)
                                                   ccGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaa                                         <  1:2870836/101‑1 (MQ=255)
                                                     gATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaa                                       >  2:337950/1‑101 (MQ=255)
                                                      aTCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaag                                      >  2:634319/1‑100 (MQ=255)
                                                      aTCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaag                                      >  1:2198123/1‑100 (MQ=255)
                                                       tCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaagg                                     >  2:277217/1‑99 (MQ=255)
                                                        cATGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggc                                    <  1:2259268/101‑4 (MQ=255)
                                                          tGTCGGCTTCGCGCTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCg                                 <  1:1955324/101‑1 (MQ=255)
                                                             cggcttcgcgcTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAgg                              >  1:1796236/1‑101 (MQ=255)
                                                               gcttcgcgcTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggcg                            >  2:749748/1‑101 (MQ=255)
                                                               gcttcgcgcTGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggcg                            <  1:3776518/101‑1 (MQ=255)
                                                                 ttcgcgctGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggcggc                          >  1:3717938/6‑101 (MQ=255)
                                                                 ttcgcgctGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGACGAggcggc                          >  1:3544621/6‑101 (MQ=255)
                                                                  tcgcgctGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGgcggcg                         <  1:2251500/97‑1 (MQ=255)
                                                                   cgcgctGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGgcgc                        <  1:4020901/98‑1 (MQ=255)
                                                                   cgcgctGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGgcgc                        >  1:407756/4‑101 (MQ=255)
                                                                    gcgctGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGACGGGCACGC.AAAAGGCCGAGGCGGCGCt                       <  2:3968449/99‑1 (MQ=255)
                                                                     cgctGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTg                      >  1:2836234/2‑101 (MQ=255)
                                                                     cgctGGCGCCGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTg                      >  2:238061/2‑101 (MQ=255)
                                                                       ctggcgccGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.GAAAGGCCGAGGCGGCGCTGaa                    <  1:4208062/95‑1 (MQ=255)
                                                                       ctggcgccGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGaa                    <  1:4245694/95‑1 (MQ=255)
                                                                         ggcgccGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAAt                  >  1:1153990/5‑101 (MQ=255)
                                                                         ggcgccGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAAt                  <  1:1673564/97‑1 (MQ=255)
                                                                          gcgccGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAAtt                 >  2:61652/4‑101 (MQ=255)
                                                                           cgccGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtc                <  2:289519/99‑1 (MQ=255)
                                                                            gccGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcg               >  1:1129031/2‑101 (MQ=255)
                                                                             ccGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgt              <  1:893826/101‑1 (MQ=255)
                                                                              cGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtc             <  2:3033654/101‑1 (MQ=255)
                                                                              cGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtc             >  1:4417498/1‑101 (MQ=255)
                                                                              cGAACAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtc             >  2:773295/1‑101 (MQ=255)
                                                                                aaCAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGa           >  1:232184/1‑101 (MQ=255)
                                                                                aaCAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGa           <  2:3323042/101‑1 (MQ=255)
                                                                                 aCAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGaa          <  2:2468954/101‑1 (MQ=255)
                                                                                 aCAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGaa          <  1:3948327/101‑1 (MQ=255)
                                                                                  cAAGGCC.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAAt         <  1:2578655/101‑1 (MQ=255)
                                                                                   aaggcc.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATg        >  2:1337586/6‑101 (MQ=255)
                                                                                    aggcc.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATgg       >  2:3657944/5‑101 (MQ=255)
                                                                                    aggcc.GGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATgg       <  2:563232/97‑1 (MQ=255)
                                                                                          ggTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGAc  <  1:1618059/101‑1 (MQ=255)
                                                                                                ||                                                                                                
ATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCG.GCTGGC.CCGAACAAGG.C.GGTGTCCGCAAGCATGGCAACTGGCTCTGGGCCTGGAACCCTCGCCAATTCCGGCGGGCACGCAAAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGAC  >  NC_014034/2180093‑2180282

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG