Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,304,900 2 bp→CA 100% coding (2674‑2675/2808 nt) RCAP_rcc02139 ← methyl‑accepting chemotaxis protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,304,9000A→C100.0% 599.4 171V892G (GTA→GGA) RCAP_rcc02139methyl‑accepting chemotaxis protein
Reads supporting (aligned to +/- strand):  new base (72/99):  ref base (0/0):  total (72/99)
*NC_0140342,304,9010C→A100.0% 591.9 171V892L (GTA→TTA) RCAP_rcc02139methyl‑accepting chemotaxis protein
Reads supporting (aligned to +/- strand):  new base (72/99):  ref base (0/0):  total (72/99)

Only 100 of 174 total aligned reads displayed.

ATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCGCCCTGCATGC.GAAGACC.G.C.GTACGCTCCCG.CA.GGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTG  >  NC_014034/2304809‑2304988
                                                                                               ||                                                                                         
aTAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCg                                                                                    >  1:610156/1‑101 (MQ=255)
 tAATCTTGCGCAAACCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.c                                                                                  <  1:515124/101‑1 (MQ=255)
  aaTCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCGTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.Ca                                                                                 >  2:2276797/1‑101 (MQ=255)
  aaTCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.Ca                                                                                 >  2:4141316/1‑101 (MQ=255)
   aTCTTGCGCAAACCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CA.g                                                                               <  1:1549174/101‑1 (MQ=255)
   aTCTTGCGCAAACCCCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CA.g                                                                               <  1:3277092/101‑1 (MQ=255)
    tCTTGCGCAAACCCGGGCCGAGTCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CA.gg                                                                              <  2:4325987/101‑1 (MQ=255)
    tCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCTGCTGGCG.CCTGCATGCGGGAGACC.GCC.GTCAGCTCCCG.CA.gg                                                                              >  2:1769368/1‑101 (MQ=255)
       tGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CA.gggtc                                                                           >  2:1660157/1‑98 (MQ=255)
       tGCGAAAACCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CA.gggtc                                                                           <  2:3330584/101‑4 (MQ=255)
        gcgcAAACCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CA.gggtct                                                                          <  2:1332496/101‑5 (MQ=255)
        gcgcAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CA.gggtct                                                                          >  2:1799642/1‑97 (MQ=255)
         cgaaaaaCGCGGCCGGACCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CA.gggtctg                                                                         <  1:109960/98‑6 (MQ=255)
           cAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTgcg                                                                        >  1:3667541/1‑101 (MQ=255)
            aaaaCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCGCCG.GGTCTgcgc                                                                       >  1:3162833/1‑101 (MQ=255)
            aaaaCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTgcgc                                                                       >  1:3915596/1‑101 (MQ=255)
              accTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCt                                                                     <  1:2506183/99‑1 (MQ=255)
                cTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCg                                                                   >  2:2646087/1‑101 (MQ=255)
                cTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGGCTGCGCCTCg                                                                   >  2:1235116/1‑101 (MQ=255)
                  cGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGcc                                                                 >  1:3887043/1‑101 (MQ=255)
                   ggCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGccc                                                                >  2:2320140/1‑101 (MQ=255)
                   ggCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGccc                                                                <  2:3348117/101‑1 (MQ=255)
                   ggCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGccc                                                                <  1:3444749/101‑1 (MQ=255)
                   ggCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGccc                                                                >  2:434479/1‑101 (MQ=255)
                    gCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCca                                                               <  2:1927523/101‑1 (MQ=255)
                    gCCGGACCCGGCCTGCCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCca                                                               <  2:1285012/101‑1 (MQ=255)
                     ccGGACCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCcag                                                              <  2:2379206/101‑1 (MQ=255)
                      cGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCcagc                                                             <  1:3389620/101‑1 (MQ=255)
                      cGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCcagc                                                             >  2:3794350/1‑101 (MQ=255)
                       ggATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCcagca                                                            <  1:1265099/101‑1 (MQ=255)
                        gtgccGGCCGGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCcagcag                                                           <  1:460213/98‑1 (MQ=255)
                        gATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCcagcag                                                           >  1:4149768/1‑101 (MQ=255)
                         aTCGGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCagcagc                                                          <  1:2962098/101‑1 (MQ=255)
                         aTCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCagcagc                                                          <  1:3688199/101‑1 (MQ=255)
                           ccGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGCGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAgcgc                                                        >  1:2731578/1‑101 (MQ=255)
                            cgcccTGTCAGCCGCCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGcc                                                       <  1:1079309/98‑1 (MQ=255)
                            cGGCGTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGcc                                                       <  1:1457026/101‑1 (MQ=255)
                             ggCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCt                                                      >  1:3044714/1‑101 (MQ=255)
                             ggCCTGTCAGCCGCCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCt                                                      <  2:3651209/101‑1 (MQ=255)
                             ggCCTGCCGGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCt                                                      <  1:3723407/101‑1 (MQ=255)
                               ccTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGc                                                    >  2:331820/1‑101 (MQ=255)
                               ccTGCCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATTCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGc                                                    <  2:1872017/101‑1 (MQ=255)
                                cTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCg                                                   >  1:688750/1‑101 (MQ=255)
                                    cAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTcgc                                               >  1:758588/1‑101 (MQ=255)
                                    cAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTcgc                                               <  2:2833590/101‑1 (MQ=255)
                                    cAGCCGCCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTcgc                                               <  2:3548080/101‑1 (MQ=255)
                                     cgCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTcgcg                                              <  1:3948868/100‑1 (MQ=255)
                                         ggCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcg                                          >  2:3943003/1‑101 (MQ=255)
                                           ccTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcgcg                                        <  2:22239/101‑1 (MQ=255)
                                           ccTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcgcg                                        <  1:3785492/101‑1 (MQ=255)
                                             tGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcgcggt                                      >  1:911119/1‑99 (MQ=255)
                                              gCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGttt                                     <  1:2320290/101‑1 (MQ=255)
                                               ccGCAACGGCGGCGCCGGTGGCG.CCTGCATTCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGtttt                                    <  1:1777363/101‑1 (MQ=255)
                                                 gCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTcc                                  <  1:2466094/101‑1 (MQ=255)
                                                  cAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCt                                 >  1:2182722/1‑101 (MQ=255)
                                                  cAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCt                                 >  2:182656/1‑101 (MQ=255)
                                                   aaCGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCTC.C.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGGTTTCCTc                                >  2:390057/1‑101 (MQ=255)
                                                   aaCGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTc                                <  1:2752991/101‑1 (MQ=255)
                                                   aaCGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTc                                <  1:1918672/101‑1 (MQ=255)
                                                   aaCGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTc                                >  2:1186997/1‑101 (MQ=255)
                                                   aaCGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGGTTTCCTc                                >  1:4253484/1‑101 (MQ=255)
                                                      ggcggcGCCGGTGGCG.CCTGCATGCGGAAGGCC.GCC.GTCAGCTGCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGaa                             <  1:288152/101‑1 (MQ=255)
                                                       gcggcgCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAAc                            <  1:3122769/101‑1 (MQ=255)
                                                       gcggcgCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAAc                            <  2:3473458/101‑1 (MQ=255)
                                                       gcggcgCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAAc                            <  2:2190246/101‑1 (MQ=255)
                                                        gggcgCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACa                           <  2:2780843/100‑1 (MQ=25)
                                                        cggcgCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACa                           >  1:1973146/1‑101 (MQ=255)
                                                        cggcgCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACa                           <  2:1900007/101‑1 (MQ=37)
                                                         ggcgCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACAt                          <  2:3915342/101‑1 (MQ=255)
                                                         ggcgCCGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACAt                          <  2:4294936/101‑1 (MQ=255)
                                                             ccGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgcc                      >  1:2965113/1‑101 (MQ=25)
                                                              cGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccg                     <  2:754918/101‑1 (MQ=25)
                                                              cGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccg                     <  1:2276942/101‑1 (MQ=25)
                                                              cGGTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccg                     <  1:817882/101‑1 (MQ=25)
                                                               ggTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccgc                    >  1:4072745/1‑101 (MQ=25)
                                                               ggTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccgc                    >  1:2710671/1‑101 (MQ=25)
                                                                gTGGCG.CCTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTCCGCCGCGGCCGCGTTTTCCTCGAACATCGCCGCa                   >  1:383511/1‑101 (MQ=25)
                                                                 tGGCG.CCTGCATGCGGAAGACC.G.CGGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAt                  <  1:1834542/101‑1 (MQ=25)
                                                                     cgccTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgt               <  1:1235209/99‑1 (MQ=25)
                                                                      gccTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgtg              >  1:3329215/2‑101 (MQ=21)
                                                                        cTGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCt            <  1:4369653/101‑1 (MQ=25)
                                                                         tGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTg           <  2:298647/101‑1 (MQ=25)
                                                                         tGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTg           <  2:3162609/101‑1 (MQ=25)
                                                                         tGCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTg           >  1:1508958/1‑101 (MQ=21)
                                                                          gCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCTGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTgg          >  1:768079/1‑101 (MQ=21)
                                                                          gCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTgg          <  2:420596/101‑1 (MQ=25)
                                                                          gCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTgg          <  1:1799761/101‑1 (MQ=25)
                                                                          gCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTgg          >  2:1340986/1‑101 (MQ=21)
                                                                          gCATGCGGAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTgg          <  1:1989373/101‑1 (MQ=25)
                                                                            atgcggAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGt        >  2:4224339/6‑101 (MQ=21)
                                                                            atgcggAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGt        <  1:4300742/96‑1 (MQ=25)
                                                                            atgcggAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCCGGGt        >  2:2017423/6‑101 (MQ=21)
                                                                             tgcggAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTg       <  2:3886789/97‑1 (MQ=25)
                                                                               cggAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCTGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGAc     <  1:2121578/99‑1 (MQ=21)
                                                                               cggAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGAc     <  2:274042/99‑1 (MQ=25)
                                                                                 gAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCt   >  2:620207/1‑101 (MQ=21)
                                                                                 gAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCt   >  2:2820692/1‑101 (MQ=21)
                                                                                 gAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCt   <  1:350596/101‑1 (MQ=21)
                                                                                 gAAGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCt   >  2:2865112/1‑101 (MQ=21)
                                                                                  aaGACC.GCC.GTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTg  <  2:1451017/101‑1 (MQ=21)
                                                                                               ||                                                                                         
ATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCGCCCTGCATGC.GAAGACC.G.C.GTACGCTCCCG.CA.GGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTG  >  NC_014034/2304809‑2304988

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG