Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,307,862 3 bp→GGC 100% coding (933‑935/1203 nt) RCAP_rcc02140 ← extracellular ligand‑binding receptor

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,307,8620C→G100.0% 27.7 146G312A (GGC→GCC) RCAP_rcc02140extracellular ligand‑binding receptor
Reads supporting (aligned to +/- strand):  new base (67/66):  ref base (12/1):  total (79/67)
Fisher's exact test strand distribution p-value = 3.31e-03
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00
*NC_0140342,307,8630C→G100.0% 527.0 146G312R (GGC→CGC) RCAP_rcc02140extracellular ligand‑binding receptor
Reads supporting (aligned to +/- strand):  new base (78/67):  ref base (1/0):  total (79/67)
*NC_0140342,307,8640G→C100.0% 28.3 146N311K (AAC→AAG) RCAP_rcc02140extracellular ligand‑binding receptor
Reads supporting (aligned to +/- strand):  new base (66/66):  ref base (12/1):  total (79/67)
Fisher's exact test strand distribution p-value = 3.14e-03
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 151 total aligned reads displayed.

TAGCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCGCCG.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCTGCG  >  NC_014034/2307772‑2307952
                                                                                          |||                                                                                         
tAGCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTcgccgc                                                                                  >  1:2952618/1‑101 (MQ=255)
  gCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGccgccg                                                                                >  2:2083031/1‑101 (MQ=255)
  gCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGccgccg                                                                                >  2:2573260/1‑101 (MQ=255)
    tGGACCGGGTCGGGTCCGCCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGa                                                                              <  1:851340/101‑1 (MQ=255)
       aaCGGGTGGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATcc                                                                           <  1:892188/101‑1 (MQ=255)
       aaCGGGTGGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCC.CGGCTTCGCCGCCGGATcc                                                                           <  1:1737227/101‑1 (MQ=255)
       aaCGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGGCGGCCGGCCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATcc                                                                           >  2:3852816/1‑101 (MQ=255)
       aaCGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATcc                                                                           >  2:1738124/1‑101 (MQ=255)
        ccgggccgggtGCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCa                                                                          <  2:119397/100‑1 (MQ=255)
        aCGGGTCGGGTTCGGCCCCGCTGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCa                                                                          >  1:3878803/1‑101 (MQ=255)
        aCGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATGCa                                                                          >  2:2192464/1‑101 (MQ=255)
          gggtcgggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAgc                                                                        <  1:238051/101‑1 (MQ=255)
          gggtcgggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAgc                                                                        <  1:2453750/101‑1 (MQ=255)
          gggtcgggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAgc                                                                        <  2:4243180/101‑1 (MQ=255)
            gtcgggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAgcgc                                                                      >  1:670759/1‑101 (MQ=255)
             tcgggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGcgcc                                                                     <  1:3644124/101‑1 (MQ=255)
              cgggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGcgccg                                                                    >  1:1640379/1‑101 (MQ=255)
              cgggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGcgccg                                                                    <  1:1353167/101‑1 (MQ=255)
               tggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGcgccgc                                                                   >  2:1686152/2‑101 (MQ=255)
               gggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGcgccgc                                                                   >  1:2223613/1‑101 (MQ=255)
                gtttCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCcgcg                                                                  <  2:2642643/99‑1 (MQ=255)
                ggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCcgcg                                                                  >  1:3598907/1‑101 (MQ=255)
                ggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCcgcg                                                                  >  2:690715/1‑101 (MQ=255)
                ggtGCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCcgcg                                                                  <  2:4082178/101‑1 (MQ=255)
                ggtCCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCcgcg                                                                  <  2:806588/101‑1 (MQ=255)
                 gtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGa                                                                 >  1:2681794/1‑101 (MQ=255)
                   tCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAATCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAgc                                                               >  1:348637/1‑101 (MQ=255)
                   tCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAgc                                                               >  1:4324769/1‑101 (MQ=255)
                   tCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAgc                                                               >  2:3091198/1‑101 (MQ=255)
                     ggCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGCTAGCCCTCGAAGGAGGCGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAgcgc                                                             >  1:4431891/1‑101 (MQ=255)
                     ggCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAgcgc                                                             >  1:3872362/1‑101 (MQ=255)
                     ggCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAgcgc                                                             >  2:1663233/1‑101 (MQ=255)
                     ggCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAgcgc                                                             <  2:1759005/101‑1 (MQ=255)
                      gCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGcgcc                                                            >  1:3155920/1‑101 (MQ=255)
                      gCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGcgcc                                                            <  1:3427547/101‑1 (MQ=255)
                       ccccGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGcgccg                                                           <  2:3011019/101‑1 (MQ=255)
                         ccgccgCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCgccgcc                                                         <  1:2102311/101‑1 (MQ=255)
                          cgccgcGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCt                                                        >  2:1043844/1‑101 (MQ=255)
                          cgccgcGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCt                                                        >  2:2802745/1‑101 (MQ=255)
                            ccgcGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGa                                                      <  1:1346138/101‑1 (MQ=255)
                             cgcgGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGAt                                                     <  1:2319291/101‑1 (MQ=255)
                             cgcgGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGAt                                                     >  2:3191388/1‑101 (MQ=255)
                             cgcgGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGAt                                                     <  1:1810042/101‑1 (MQ=255)
                             cgcgGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGAt                                                     >  1:3093219/1‑101 (MQ=255)
                              gcgGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATa                                                    <  1:2926814/101‑1 (MQ=255)
                              gcgGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATa                                                    <  1:943315/101‑1 (MQ=255)
                               cgGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAg                                                   <  1:3765471/101‑1 (MQ=255)
                                ggCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAgc                                                  <  2:2016531/101‑1 (MQ=255)
                                  ccagcgccgccagcgccAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAgcgc                                                >  1:2860466/1‑101 (MQ=255)
                                        ccgccagcgccAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGccacc                                          >  1:43095/1‑101 (MQ=255)
                                        ccgccagcgccAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGccacc                                          >  2:4023411/1‑101 (MQ=255)
                                          gccagcgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCcaccac                                        <  2:1472032/101‑1 (MQ=255)
                                            cagcgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCg                                      >  1:207324/1‑101 (MQ=255)
                                            cagcgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCg                                      >  2:4367058/1‑101 (MQ=255)
                                             cgcgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCgg                                     <  2:3788055/100‑1 (MQ=255)
                                             agggccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCGCCACCgg                                     <  1:2622035/98‑1 (MQ=255)
                                             agcgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCgg                                     <  2:2130233/101‑1 (MQ=255)
                                              gcgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGa                                    <  1:946918/101‑1 (MQ=255)
                                                gccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAAt                                  >  1:2758068/1‑101 (MQ=255)
                                                gccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAAt                                  <  1:1465860/101‑1 (MQ=255)
                                                 ccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATg                                 >  2:1387286/1‑101 (MQ=255)
                                                 ccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATg                                 >  2:2983395/1‑101 (MQ=255)
                                                 ccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATg                                 <  1:2513560/101‑1 (MQ=255)
                                                   agACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTc                               <  1:1431990/101‑1 (MQ=255)
                                                   agACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTc                               >  1:3218854/1‑101 (MQ=255)
                                                    gACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGCTCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGCATGTCg                              >  1:3065459/1‑101 (MQ=255)
                                                    gACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCg                              <  2:2952008/101‑1 (MQ=255)
                                                       ggCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGgtg                           >  2:1430632/1‑101 (MQ=255)
                                                          cGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCg                        <  1:2520834/101‑1 (MQ=255)
                                                          cGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGCCg                        <  1:2555012/101‑1 (MQ=255)
                                                          cGGTCAGATAGCCCTCGAAGGAGACGAAGCC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCg                        >  1:3432068/1‑101 (MQ=255)
                                                           ggTCAGATAGCCCTCGAAGGAGACGACGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGACCGCGGCCTGATAGGGCGCCCCCACCGGAATGTCGGTGTCgc                       >  2:2915964/1‑100 (MQ=255)
                                                           ggTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGa                       <  1:3623848/101‑1 (MQ=255)
                                                            ggccgATAGCCCTCGAAGGACCATACGCCCGGC.TTCGCCCGCGGATCCCGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGGCGaa                      <  2:3683369/97‑1 (MQ=255)
                                                            gTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGaa                      <  1:3961150/101‑1 (MQ=255)
                                                            gTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGaa                      >  1:3758427/1‑101 (MQ=255)
                                                            gTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGaa                      <  1:1607177/101‑1 (MQ=255)
                                                            gTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGaa                      >  2:946031/1‑101 (MQ=255)
                                                            gTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCACCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGaa                      >  2:1626402/1‑101 (MQ=255)
                                                             tCAGATAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAAc                     >  1:4335791/1‑101 (MQ=255)
                                                             tCAGATAGCCCTCGAAGGAGACGAAGCC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAAc                     >  1:1775421/1‑101 (MQ=255)
                                                              cAGATAGCCCTCGAAGGAGACGAAGCC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACg                    >  1:1034504/1‑101 (MQ=255)
                                                                  tAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATa                >  1:4200402/1‑101 (MQ=255)
                                                                  tAGCCCTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATa                <  2:1625357/101‑1 (MQ=255)
                                                                  tAGCCCTCGAAGGAGACGAAGCC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATa                >  2:2333620/1‑101 (MQ=255)
                                                                   aGCCCTCGAAGGAGACGAAGCC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAg               >  1:296842/1‑101 (MQ=255)
                                                                      ccTCGAAGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGc            <  2:1524375/101‑1 (MQ=255)
                                                                      ccTCGAAGGAGACGAAGCC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGc            >  2:2261283/1‑101 (MQ=255)
                                                                        tCGAAGGAGACGAAGCC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCAc          >  1:3204342/1‑101 (MQ=255)
                                                                           aaGGAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGAc       >  2:3236291/1‑101 (MQ=255)
                                                                            aGGAGACGAAGCC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGAcc      >  1:3167575/1‑101 (MQ=255)
                                                                             ggAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCt     <  2:3598659/101‑1 (MQ=255)
                                                                             ggAGACGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCt     >  1:612858/1‑101 (MQ=255)
                                                                              gagaCGAAGCCGGGG.TTCGCTGGCGGATCGAGCGCCGAGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGGCGAACGGATAGGGCACGACCTg    <  2:3074564/101‑1 (MQ=255)
                                                                              gagaCGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCGCCGGAATGTCGGTGTCGAACGGATAGGGCACGACCTg    <  1:681119/101‑1 (MQ=255)
                                                                               agaCGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGCCCtcc   >  2:1939090/1‑99 (MQ=255)
                                                                               agaCGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCTGc   >  2:3470267/1‑101 (MQ=255)
                                                                               agaCGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCTGc   <  1:2149368/101‑1 (MQ=255)
                                                                                gaCGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCTGCg  <  2:4160294/101‑1 (MQ=255)
                                                                                gaCGAAGCCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCTGCg  <  1:1259220/101‑1 (MQ=255)
                                                                                          |||                                                                                         
TAGCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCGCCG.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCTGCG  >  NC_014034/2307772‑2307952

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG