Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,620,690 2 bp→CT 100% coding (1050‑1051/1188 nt) livJ → branched‑chain amino acid ABC transporter periplasmic branched‑chain amino acid‑binding protein LivJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,620,6900T→C100.0% 852.1 220P350P (CCT→CCClivJbranched‑chain amino acid ABC transporter periplasmic branched‑chain amino acid‑binding protein LivJ
Reads supporting (aligned to +/- strand):  new base (95/125):  ref base (0/0):  total (95/125)
*NC_0140342,620,6910A→T100.0% 860.6 220N351Y (AAC→TAC) livJbranched‑chain amino acid ABC transporter periplasmic branched‑chain amino acid‑binding protein LivJ
Reads supporting (aligned to +/- strand):  new base (95/125):  ref base (0/0):  total (95/125)

Only 100 of 223 total aligned reads displayed.

GAATATATCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCTAACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGAATACAA  >  NC_014034/2620595‑2620785
                                                                                               ||                                                                                              
gAATATATCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGGTTCGCCGCGCCGACCCCCTACAt                                                                                            >  1:2953326/1‑101 (MQ=255)
 aaTATATCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATg                                                                                           <  2:1783151/101‑1 (MQ=255)
 aaTATATCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATg                                                                                           <  1:2264328/101‑1 (MQ=255)
 aaTATATCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATg                                                                                           >  1:1260786/1‑101 (MQ=255)
  atctatCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGa                                                                                          <  2:2417585/98‑1 (MQ=255)
  atatatCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGa                                                                                          <  2:2681498/101‑1 (MQ=255)
  atatatCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCNNCATGa                                                                                          >  1:1871221/1‑101 (MQ=255)
   tccgaccTGCAGACGCTGAAAGCCGCTACGAAGGGCCTGACCGACATTGCCGCCTTCAAGACAGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGaa                                                                                         <  2:1989928/96‑1 (MQ=255)
   tatatCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGaa                                                                                         >  1:1454819/1‑101 (MQ=255)
    atatCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAg                                                                                        >  2:2458610/1‑101 (MQ=255)
     tatCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAgg                                                                                       >  2:352078/1‑101 (MQ=255)
     tatCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAgg                                                                                       >  1:4065434/1‑101 (MQ=255)
     tatCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAgg                                                                                       <  1:3936619/101‑1 (MQ=255)
      atCCTGCAGACGGTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAgat                                                                                      >  1:2417742/1‑99 (MQ=255)
       tCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGc                                                                                     >  2:4397326/1‑101 (MQ=255)
        ccTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCg                                                                                    <  1:3089000/101‑1 (MQ=255)
        ccTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCg                                                                                    <  2:565097/101‑1 (MQ=255)
         cTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGa                                                                                   >  2:2009838/1‑101 (MQ=255)
         cTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGa                                                                                   >  2:1940710/1‑101 (MQ=255)
         cTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGa                                                                                   >  1:699181/1‑101 (MQ=255)
         cTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGa                                                                                   >  1:1835527/1‑101 (MQ=255)
          tGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGAt                                                                                  <  2:3824115/101‑1 (MQ=255)
          tGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGAt                                                                                  <  2:1815290/101‑1 (MQ=255)
           gCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATg                                                                                 <  2:2342218/101‑1 (MQ=255)
           gCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATg                                                                                 <  2:2318760/101‑1 (MQ=255)
           gCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATg                                                                                 <  1:17991/101‑1 (MQ=255)
            cAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGc                                                                                >  2:3547795/1‑101 (MQ=255)
            cAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGc                                                                                <  1:3769896/101‑1 (MQ=255)
             aGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCg                                                                               <  1:479720/101‑1 (MQ=255)
              gACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGa                                                                              >  2:846978/1‑101 (MQ=255)
               aCGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGaa                                                                             <  1:1416146/101‑1 (MQ=255)
               aCGCTGAAAGACGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGaa                                                                             <  1:3587641/101‑1 (MQ=255)
                cGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTGCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAg                                                                            >  2:2274116/1‑101 (MQ=255)
                  cTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCt                                                                          <  1:3204109/101‑1 (MQ=255)
                   tGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTg                                                                         >  1:1147656/1‑101 (MQ=255)
                   tGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTg                                                                         <  2:418886/101‑1 (MQ=255)
                   tGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTg                                                                         >  2:919166/1‑101 (MQ=255)
                   ggAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTg                                                                         <  1:3387674/100‑1 (MQ=255)
                    gAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGa                                                                        <  1:3457172/101‑1 (MQ=255)
                     aaaGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAg                                                                       <  1:1198513/101‑1 (MQ=255)
                      aaGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGc                                                                      <  2:2059398/101‑1 (MQ=255)
                      aaGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGc                                                                      <  2:2649602/101‑1 (MQ=255)
                      aaGCCGATCCGAAGGGCCTGACCGACATTGCCCCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGTGATGccctgcttttc                                                                      <  2:2151771/101‑11 (MQ=255)
                       aGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCt                                                                     <  1:1765389/101‑1 (MQ=255)
                        gCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTa                                                                    >  1:2861767/1‑101 (MQ=255)
                         ccGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTAt                                                                   <  2:3373959/101‑1 (MQ=255)
                         ccGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTAt                                                                   <  2:1246969/101‑1 (MQ=255)
                          gatctcGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATg                                                                  <  2:813810/96‑1 (MQ=255)
                          cGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATg                                                                  <  1:1388532/101‑1 (MQ=255)
                           gATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGt                                                                 <  1:2985036/101‑1 (MQ=255)
                               cGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATgagatc                                                             >  1:813892/1‑96 (MQ=255)
                                gAAGGGCCTGACCGACATTGCCTCCTTCAAGACCGCGAGCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGca                                                            <  2:2027234/101‑1 (MQ=255)
                                gAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGca                                                            <  1:912469/101‑1 (MQ=255)
                                 aaGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGcac                                                           >  1:1574148/1‑101 (MQ=255)
                                 aaGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGcac                                                           >  1:2190243/1‑101 (MQ=255)
                                  aGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGcacc                                                          <  2:475058/101‑1 (MQ=255)
                                    ggCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGcaccac                                                        <  2:1216870/101‑1 (MQ=255)
                                     gccttcccGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAGCCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCaccacc                                                       <  1:1007643/95‑1 (MQ=255)
                                       cTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTc                                                     >  1:268084/1‑101 (MQ=255)
                                          aCCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGtt                                                  >  2:3687526/1‑101 (MQ=255)
                                           ccGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTc                                                 >  2:3031007/1‑101 (MQ=255)
                                            cGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCg                                                >  2:1027211/1‑101 (MQ=255)
                                            cGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCg                                                <  1:4367170/101‑1 (MQ=255)
                                             gACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCgg                                               <  1:3051444/101‑1 (MQ=255)
                                                aTTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCa                                            <  2:4158844/101‑1 (MQ=255)
                                                 ttGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAg                                           >  1:1982717/1‑101 (MQ=255)
                                                    ccgccTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAg                                        >  1:4131230/1‑101 (MQ=255)
                                                     cgccTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGc                                       <  1:2014834/101‑1 (MQ=255)
                                                     cgccTTCAAGACCGCGATCGACAGGTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGc                                       <  2:1454712/101‑1 (MQ=255)
                                                        cTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGc                                    <  2:1892602/101‑1 (MQ=255)
                                                          tCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAg                                  >  2:2895018/1‑101 (MQ=255)
                                                           cAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGc                                 >  1:3153563/1‑101 (MQ=255)
                                                            aaGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCt                                <  1:2464634/101‑1 (MQ=255)
                                                             aGACCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTg                               <  2:3501581/101‑1 (MQ=255)
                                                               aCCGCGATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTc                             <  2:2090602/101‑1 (MQ=255)
                                                                  gcgATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGt                          >  1:2566802/1‑101 (MQ=255)
                                                                    gATCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGGGc                        >  2:521877/1‑101 (MQ=255)
                                                                     aTCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGcc                       <  1:229853/101‑1 (MQ=255)
                                                                     aTCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGcc                       >  2:770352/1‑101 (MQ=255)
                                                                      tCGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCg                      <  1:1710484/101‑1 (MQ=255)
                                                                       cGACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGc                     >  1:643621/1‑101 (MQ=255)
                                                                        gACAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCt                    <  1:4125909/101‑1 (MQ=255)
                                                                         aCAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTc                   <  2:1368792/101‑1 (MQ=255)
                                                                          cAGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACGTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCg                  >  1:3100405/1‑101 (MQ=255)
                                                                           aGTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCgg                 >  1:2506373/1‑100 (MQ=255)
                                                                            gTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCgtg                >  1:106608/1‑101 (MQ=255)
                                                                            gTTTCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCgtg                <  2:3176255/101‑1 (MQ=255)
                                                                             tttCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCgtgg               >  2:4249351/1‑101 (MQ=255)
                                                                              ttCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCgtggt              <  2:64678/101‑1 (MQ=255)
                                                                               tCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCgtggtg             <  1:64952/101‑1 (MQ=255)
                                                                               tCGCCGCGCCGAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGACACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCgtggtg             <  2:2572820/101‑1 (MQ=255)
                                                                                cgccgcgccgAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGa            <  2:1565217/101‑1 (MQ=255)
                                                                                cgccgcgccgAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGa            >  2:1660738/1‑101 (MQ=255)
                                                                                 gccgcgccgAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGaa           <  2:4045452/101‑1 (MQ=255)
                                                                                 gccgcgccgAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGaa           <  1:2009967/101‑1 (MQ=255)
                                                                                  ccgcgccgAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAAc          >  1:280710/1‑101 (MQ=255)
                                                                                    gcgccgAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGa        >  1:2725712/1‑101 (MQ=255)
                                                                                    gcgccgAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGGGCCGCTCGTGGTGAACGa        >  1:2437590/1‑101 (MQ=255)
                                                                                       ccgAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGGGAACGAATa     >  2:3237768/1‑101 (MQ=255)
                                                                                         gAACCCCTACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGAATACa   >  1:4301560/1‑101 (MQ=255)
                                                                                               ||                                                                                              
GAATATATCCTGCAGACGCTGAAAGCCGATCCGAAGGGCCTGACCGACATTGCCGCCTTCAAGACCGCGATCGACAGTTTCGCCGCGCCGAACCCTAACATGAAGGGCGATGCGAAGCTGAGCTATGTCGGCACCACCTCGTTCGGGCAGAAGCGGCAGCTGTCGGTGCCGCTCGTGGTGAACGAATACAA  >  NC_014034/2620595‑2620785

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG