Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,747,876	2 bp→GC	100%	intergenic (‑208/+84)	RCAP_rcc02553 ← / ← RCAP_rcc02554	hypothetical protein/NmrA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,747,876	0	C→G	100.0%	57.7	23	intergenic (‑208/+85)	RCAP_rcc02553/RCAP_rcc02554	hypothetical protein/NmrA family protein
Reads supporting (aligned to +/- strand):  new base (21/1):  ref base (1/0):  total (22/1)
*	NC_014034	2,747,877	0	T→C	100.0%	73.0	27	intergenic (‑209/+84)	RCAP_rcc02553/RCAP_rcc02554	hypothetical protein/NmrA family protein
Reads supporting (aligned to +/- strand):  new base (25/1):  ref base (0/0):  total (26/1)

CGCCCGGCCC.GCGGCCGAGAAGCGCCCGCC.GG..CCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTCATGCCAG  >  NC_014034/2747840‑2747969                                         ||                                                                                             cgcCCGGCCCGGCGGGCGAGAAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGc                                 >  2:4142135/1‑101 (MQ=18)  gcccggcccgGCGGGCGAGAAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCg                                >  1:490430/1‑101 (MQ=18)    ccggcccgGCGGGCGAGAAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGAt                              >  1:1645388/1‑101 (MQ=18)      ggcccgGCGGGCGAGAAGCGCCCGCC.GGGACCGGC.GCTGCCCGAGGGCCTTTGGGCCCTCGGGGGGCGCAGGCCCTCTGTGACGCATGGCCAAGGCGATgg                            >  1:3661614/1‑101 (MQ=18)        cccggcgGGCGAGAAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGcc                          >  2:1361967/5‑101 (MQ=18)         ccggcgGGCGAGAAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCACGGCGATGGCCt                         >  2:3374190/4‑101 (MQ=18)             cgGGCGAGAAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGCTGGCCTTGGc                     >  2:1885540/1‑101 (MQ=21)              ggggggggaGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGcc                    <  1:4194455/93‑1 (MQ=21)              gggcGAGAAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGcc                    >  2:1409004/4‑101 (MQ=21)              gggcGAGAAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGcc                    >  1:119572/4‑101 (MQ=21)               ggcGAGAAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCa                   >  1:3600466/3‑101 (MQ=21)               ggcGAGAAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCa                   >  2:1498111/3‑101 (MQ=21)                  gagaAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAgg                >  1:475572/1‑101 (MQ=21)                  gagaAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAgg                >  1:198/1‑101 (MQ=21)                   agaAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGt               >  2:4325000/1‑101 (MQ=21)                   agaAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGt               >  1:2458491/1‑101 (MQ=21)                   agaAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGt               >  1:3026178/1‑101 (MQ=21)                   agaAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGt               >  1:3857312/1‑101 (MQ=21)                    gaAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTc              >  2:376633/1‑101 (MQ=21)                    gaAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTc              >  2:4235120/1‑101 (MQ=21)                    gaAGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGGCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAggcg              >  2:2084552/1‑99 (MQ=21)                     aaGCGCCCGCCGGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCa             >  2:430159/1‑101 (MQ=21)                         gcccgccgGG..CCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCATAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTc         >  1:1958903/1‑101 (MQ=18)                            cgccggg..ccgggCGCTGCCCGCGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTCAtc      >  2:4265707/6‑100 (MQ=21)                             gccggg..ccgggCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTCATGc     >  2:2317795/5‑101 (MQ=255)                              ccggg..ccgggCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTCATGcc    >  1:3307349/4‑101 (MQ=255)                                ggg..ccgggCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACTCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTCATGCCAg  >  1:323647/2‑101 (MQ=255)                                         ||                                                                                             CGCCCGGCCC.GCGGCCGAGAAGCGCCCGCC.GG..CCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGCCAAGGTCAGCTCATGCCAG  >  NC_014034/2747840‑2747969 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG