Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,394,235 2 bp→GC 100% coding (787‑788/831 nt) RCAP_rcc03194 ← hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,394,2350C→G100.0% 648.9 178R263P (CGG→CCG) RCAP_rcc03194hypothetical protein
Reads supporting (aligned to +/- strand):  new base (96/81):  ref base (0/1):  total (96/82)
*NC_0140343,394,2360G→C100.0% 661.2 179R263G (CGG→GGG) RCAP_rcc03194hypothetical protein
Reads supporting (aligned to +/- strand):  new base (96/81):  ref base (0/2):  total (96/83)

Only 100 of 195 total aligned reads displayed.

CGGGCGGGACCTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCCG.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCCGACCCAGCGTGCCAAGCGCCTTGCCCAGTTCCA  >  NC_014034/3394141‑3394331
                                                                                              ||                                                                                                
cgggcgggACCTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCtt                                                                                            >  1:2242880/1‑101 (MQ=255)
cgggcgggACCTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCtt                                                                                            <  2:1190212/101‑1 (MQ=255)
 gggcgggACCTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTc                                                                                           <  1:1191234/101‑1 (MQ=255)
  ggcgggACCTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTcc                                                                                          <  2:2715508/101‑1 (MQ=255)
  ggcgggACCTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTcc                                                                                          <  2:74185/101‑1 (MQ=255)
  ggcgggACCTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTcc                                                                                          <  2:1285829/101‑1 (MQ=255)
   gcgggCCCTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCg                                                                                         <  2:1626917/101‑1 (MQ=255)
    cgggACCTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGa                                                                                        <  1:1865165/101‑1 (MQ=255)
    cgggACCTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGa                                                                                        >  2:1335609/1‑101 (MQ=255)
    cgggACCTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCCTGCCCGC.CGCTTCCGa                                                                                        >  2:245532/1‑101 (MQ=255)
     gggCCCTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAg                                                                                       <  2:4057234/101‑1 (MQ=255)
     gggACCTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAg                                                                                       >  2:501614/1‑101 (MQ=255)
     gggACCTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAg                                                                                       >  1:605062/1‑101 (MQ=255)
     gggACCTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAg                                                                                       >  2:3601491/1‑101 (MQ=255)
       gACCACCCCGGTCAGCAAGGCCGCGCGCAGAGCGTCGGGGTCGGTCCCGGGCAGCAAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGcc                                                                                     <  2:2553453/101‑1 (MQ=255)
         ccTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCgg                                                                                   <  1:2402182/101‑1 (MQ=255)
          cTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCGGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCggg                                                                                  >  1:3764412/1‑101 (MQ=255)
           tCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGa                                                                                 >  2:1197749/1‑101 (MQ=255)
              ccGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAAc                                                                              >  1:2492522/1‑101 (MQ=255)
                gcgcAGCAGGCCCGCGCGCAGAGCGTCAGGGTCGTTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACcg                                                                            <  2:1580319/101‑1 (MQ=255)
                gcgcAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACcg                                                                            >  2:1419775/1‑101 (MQ=255)
                  gcagcaAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACcgcg                                                                          <  2:174079/101‑1 (MQ=255)
                  gcagcaAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACcgcg                                                                          <  1:1002990/101‑1 (MQ=255)
                   cagcaAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCgcgc                                                                         >  2:122317/1‑101 (MQ=255)
                   cagcaAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCCTCCGAGCCGGGAAACCgcgc                                                                         >  1:767059/1‑101 (MQ=255)
                    agcgAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGcc                                                                        >  2:3771857/1‑101 (MQ=255)
                    agcaAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGcc                                                                        >  2:4158247/1‑101 (MQ=255)
                    agcaAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGcc                                                                        >  1:1142905/1‑101 (MQ=255)
                     gcaAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCg                                                                       >  2:146431/1‑101 (MQ=255)
                      caAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCTCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCgg                                                                      >  1:353608/1‑101 (MQ=255)
                      caAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCgg                                                                      >  1:3154542/1‑101 (MQ=255)
                      caAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCgg                                                                      <  1:513475/101‑1 (MQ=255)
                       aaGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGa                                                                     >  1:3062725/1‑101 (MQ=255)
                       aaGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGa                                                                     >  1:686787/1‑101 (MQ=255)
                        aGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGaa                                                                    <  1:1633815/101‑1 (MQ=255)
                          gCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAAcc                                                                  <  1:3670989/101‑1 (MQ=255)
                            cgcgcgCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTc                                                                <  1:657029/101‑1 (MQ=255)
                            cgcgcgCAGAGCGTCAGGGTCGGTCACGGCCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTc                                                                <  2:1981981/101‑1 (MQ=255)
                              cgcgcAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCgg                                                              >  1:579076/1‑101 (MQ=255)
                               gcgcAGAGCGACGGGGTCGGTCCGGGGCAGCAAAGCGCCCCCTGCCCCGCCGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGt                                                             <  1:2706219/101‑1 (MQ=255)
                                ctaagggCGGCAGGGTCGGTCACGGGCAGCCAAGCTACGCCGGCGCCGCAGCCCCCTGGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTc                                                            <  1:1046640/95‑1 (MQ=255)
                                cgcAGAGCGTCGGGGTCGGCCACGGGCAGCCAAGCGCCGCCTGCGCCGCAGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTc                                                            <  2:3821707/101‑1 (MQ=255)
                                cgcAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTc                                                            >  1:4276876/1‑101 (MQ=255)
                                cgcAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTc                                                            >  2:1545913/1‑101 (MQ=255)
                                cgcAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTc                                                            >  1:2897257/1‑101 (MQ=255)
                                 gcAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCa                                                           <  1:4121601/101‑1 (MQ=255)
                                        gTCGGGGTCGGCCCGGGGCAGCCAAGCGCCGCCTGCCCCGCAGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTcgc                                                    <  1:3230648/101‑1 (MQ=255)
                                          cAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCgcgc                                                  >  1:497800/1‑101 (MQ=255)
                                          cAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCgcgc                                                  >  2:594354/1‑101 (MQ=255)
                                          cAGGGTCGGCCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCgcgc                                                  <  1:158458/101‑1 (MQ=255)
                                           aGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCa                                                 >  2:2136527/1‑101 (MQ=255)
                                               tcggtcACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGcc                                             >  1:1185123/1‑101 (MQ=255)
                                               tcggccACGGGCAGCCAAGCGCCGCCGGCCCCGCAGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGcc                                             <  1:2499056/101‑1 (MQ=255)
                                                cggtcTCGGGGAGCCAAGCGACGCCTGCGACGCAGCCACATTGCCCGC.CGCTTCTGATCCGGGCAACCGCGCCGGAACCTCGGGCAGGGCCGCGCAGGCCt                                            >  2:3675809/1‑101 (MQ=255)
                                                 tctcACGGGCACCCAAGCGACGCCTGCCCCGCAGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTc                                           <  1:948133/99‑1 (MQ=255)
                                                 ggtcAGGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTCCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTc                                           <  1:3696958/101‑1 (MQ=255)
                                                 ggtcACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTc                                           >  1:1491445/1‑101 (MQ=255)
                                                 ggccACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTc                                           <  2:178393/98‑1 (MQ=255)
                                                   tcACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCgg                                         >  2:3369637/1‑101 (MQ=255)
                                                    ccggggCAGCAAGGCGCCGCTGGGGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGt                                        <  2:4424521/98‑1 (MQ=255)
                                                       gggCACCCAGGCCACCCCTGCGCCGCAGCCACCTTCCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCtt                                     <  1:1702787/101‑1 (MQ=255)
                                                        ggCGGCCAGGCGCCGCCTGCGCCGCAGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTc                                    <  2:1993217/101‑1 (MQ=255)
                                                         gCAGCCACGCGGCCCCGGCCCCGCAGCCGCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTct                                   <  1:3601739/101‑2 (MQ=255)
                                                           aGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGa                                 >  2:2035584/1‑101 (MQ=255)
                                                           aGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGa                                 >  2:679882/1‑101 (MQ=255)
                                                           aGCCAAGCGACGCCTGCCCCGCAGCCCCCTTCCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGa                                 <  1:2608985/101‑1 (MQ=255)
                                                            gCCAAGGGCCGCCTGCGCCGCAGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGAc                                <  1:734497/101‑1 (MQ=255)
                                                             ccAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGAcc                               >  2:2335594/1‑101 (MQ=255)
                                                              caggCGCCGCCGCCGCCGCGGCCCCCTTCCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGAccc                              <  1:1335706/98‑1 (MQ=255)
                                                              cAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGAccc                              >  1:611589/1‑101 (MQ=255)
                                                               aaGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCa                             >  2:566274/1‑101 (MQ=255)
                                                                 gCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGc                           >  2:2382220/1‑101 (MQ=255)
                                                                 gCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGc                           >  1:2090930/1‑101 (MQ=255)
                                                                  cGACCCGTGCCCCGCAGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCg                          <  2:272289/101‑1 (MQ=255)
                                                                   gACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGt                         >  1:1940961/1‑101 (MQ=255)
                                                                    aCGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTg                        >  2:1015297/1‑101 (MQ=255)
                                                                       ccggccccgcAGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCa                     <  1:3752693/95‑1 (MQ=255)
                                                                       ccTGCGCCGCGGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCa                     <  2:4220827/101‑1 (MQ=255)
                                                                         tGCCCCGCACCCCCCTTCCCCGC.CGCTTCCGAGACGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAg                   <  1:3817402/101‑1 (MQ=255)
                                                                          gcgcCGCACCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAgc                  <  2:2861631/101‑1 (MQ=255)
                                                                           ccccgcACCCCCCTTCCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAgcg                 <  1:1541081/99‑1 (MQ=255)
                                                                            gccgcAGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAgcgc                <  1:3691894/101‑1 (MQ=255)
                                                                             ccgcAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGcc               >  1:2670954/1‑101 (MQ=255)
                                                                              cgcAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCt              >  2:889199/1‑101 (MQ=255)
                                                                              cgcAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCt              >  2:348570/1‑101 (MQ=255)
                                                                               gcAGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCtt             <  1:1986522/101‑1 (MQ=255)
                                                                                caccccccTTCCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCTTg            <  2:3198275/95‑1 (MQ=255)
                                                                                  gCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCTTGcc          >  1:3488764/1‑101 (MQ=255)
                                                                                  cccACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCTTGcc          <  2:346032/100‑1 (MQ=255)
                                                                                   ccACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCTTGccc         <  2:4276607/101‑1 (MQ=255)
                                                                                   ccACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCTTGccc         >  1:4405614/1‑101 (MQ=255)
                                                                                   ccACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCTTGccc         >  1:1657624/1‑101 (MQ=255)
                                                                                   ccACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCTTGccc         >  1:1213974/1‑101 (MQ=255)
                                                                                    ccccTTCCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCTTGCCCa        <  1:934229/99‑1 (MQ=255)
                                                                                      ccTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCTTGCCCAGt      >  2:3527808/1‑101 (MQ=255)
                                                                                      ccTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCTTGCCCAGt      >  2:3058409/1‑101 (MQ=255)
                                                                                       cTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCTTGCCCAGtt     >  2:796006/1‑101 (MQ=255)
                                                                                        ttGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCTTGCCCAGTTc    >  2:1554614/1‑101 (MQ=255)
                                                                                          cccccGCCGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCTTGCCCAGTTcc   <  1:1015382/100‑1 (MQ=255)
                                                                                           ccccGCCGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCTTGCCCAGTTCca  <  2:3979371/101‑1 (MQ=255)
                                                                                              ||                                                                                                
CGGGCGGGACCTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGTCGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCCG.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCCGACCCAGCGTGCCAAGCGCCTTGCCCAGTTCCA  >  NC_014034/3394141‑3394331

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG