Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 774,622 2 bp→TC 100% intergenic (‑76/+43) oppB1 ← / ← oppA1 oligopeptide ABC transporter permease OppB/oligopeptide ABC transporter periplasmic oligopeptide‑binding protein OppA

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034774,6220G→T100.0% 10.7 3intergenic (‑76/+44)oppB1/oppA1oligopeptide ABC transporter permease OppB/oligopeptide ABC transporter periplasmic oligopeptide‑binding protein OppA
Reads supporting (aligned to +/- strand):  new base (3/0):  ref base (0/0):  total (3/0)
*NC_014034774,6230G→C100.0% 10.7 3intergenic (‑77/+43)oppB1/oppA1oligopeptide ABC transporter permease OppB/oligopeptide ABC transporter periplasmic oligopeptide‑binding protein OppA
Reads supporting (aligned to +/- strand):  new base (3/0):  ref base (0/0):  total (3/0)

CGGGCCGCAGCGCCCCCCCCCCCCTGGGGAACCGGCCCTGCCGGT.CGA.CGTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATT  >  NC_014034/774596‑774703
                          ||                                                                                  
gcaggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCCGGTCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGt           >  2:420557/10‑101 (MQ=255)
 caggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCCGGTCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGtt          >  1:2718900/9‑101 (MQ=255)
         gcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCCGGTCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTAtg  >  2:1383497/1‑100 (MQ=255)
                          ||                                                                                  
CGGGCCGCAGCGCCCCCCCCCCCCTGGGGAACCGGCCCTGCCGGT.CGA.CGTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATT  >  NC_014034/774596‑774703

Base quality scores:  ATCG  < 3 ≤  ATCG  < 10 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 38 ≤  ATCG