Organism : Bacillus cereus ATCC14579 | Module List :
BC4326

Methyltransferase (NCBI ptt file)

CircVis
Functional Annotations (12)
Function System
SAM-dependent methyltransferases cog/ cog
metabolic process go/ biological_process
methyltransferase activity go/ molecular_function
lipid biosynthetic process go/ biological_process
cyclopropane-fatty-acyl-phospholipid synthase activity go/ molecular_function
oligosaccharide biosynthetic process go/ biological_process
nodulation go/ biological_process
Histidine metabolism kegg/ kegg pathway
Tyrosine metabolism kegg/ kegg pathway
Selenocompound metabolism kegg/ kegg pathway
Polycyclic aromatic hydrocarbon degradation kegg/ kegg pathway
Microbial metabolism in diverse environments kegg/ kegg pathway
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for BC4326
(Mouseover regulator name to see its description)

BC4326 is regulated by 30 influences and regulates 0 modules.
Regulators for BC4326 (30)
Regulator Module Operator
BC0051 259 tf
BC0122 259 tf
BC0123 259 tf
BC0477 259 tf
BC1033 259 tf
BC1337 259 tf
BC1363 259 tf
BC1996 259 tf
BC2680 259 tf
BC2760 259 tf
BC3493 259 tf
BC3653 259 tf
BC3814 259 tf
BC3826 259 tf
BC3982 259 tf
BC5265 259 tf
BC0059 236 tf
BC0116 236 tf
BC0122 236 tf
BC1363 236 tf
BC1477 236 tf
BC2442 236 tf
BC2670 236 tf
BC3826 236 tf
BC3844 236 tf
BC4010 236 tf
BC4057 236 tf
BC5265 236 tf
BC5332 236 tf
BC5339 236 tf

Warning: BC4326 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
4388 8.00e+00 tgaAgGgGaGaTTTT
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4389 7.10e+01 tATgA.gtgGaaaaa
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4434 3.10e+01 ctcTtcttTgttaac.aagGg
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4435 5.70e+02 GAggTG
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for BC4326

BC4326 is enriched for 12 functions in 3 categories.
Enrichment Table (12)
Function System
SAM-dependent methyltransferases cog/ cog
metabolic process go/ biological_process
methyltransferase activity go/ molecular_function
lipid biosynthetic process go/ biological_process
cyclopropane-fatty-acyl-phospholipid synthase activity go/ molecular_function
oligosaccharide biosynthetic process go/ biological_process
nodulation go/ biological_process
Histidine metabolism kegg/ kegg pathway
Tyrosine metabolism kegg/ kegg pathway
Selenocompound metabolism kegg/ kegg pathway
Polycyclic aromatic hydrocarbon degradation kegg/ kegg pathway
Microbial metabolism in diverse environments kegg/ kegg pathway
Module neighborhood information for BC4326

BC4326 has total of 27 gene neighbors in modules 236, 259
Gene neighbors (27)
Gene Common Name Description Module membership
BC1504 BC1504 GTP-binding protein (NCBI ptt file) 49, 259
BC1505 BC1505 Glycerol-3-phosphate dehydrogenase [NAD(P)+] (NCBI ptt file) 49, 259
BC1563 BC1563 Methyltransferase (NCBI ptt file) 94, 259
BC1564 BC1564 hypothetical protein (NCBI ptt file) 94, 259
BC2102 BC2102 hypothetical protein (NCBI ptt file) 69, 236
BC2103 BC2103 ATP-dependent RNA helicase (NCBI ptt file) 69, 236
BC2933 BC2933 ABC transporter ATP-binding protein (NCBI ptt file) 236, 423
BC3542 BC3542 Transcriptional regulator (NCBI ptt file) 66, 236
BC3681 BC3681 IG hypothetical 18106 (NCBI ptt file) 182, 236
BC3707 BC3707 Aluminum resistance protein (NCBI ptt file) 236, 283
BC3817 BC3817 Prolyl-tRNA synthetase (NCBI ptt file) 14, 236
BC3830 BC3830 Glucose inhibited division protein A (NCBI ptt file) 94, 236
BC4326 BC4326 Methyltransferase (NCBI ptt file) 236, 259
BC4327 BC4327 iojap protein family (NCBI ptt file) 236, 259
BC4328 BC4328 hydrolase (HAD superfamily) (NCBI ptt file) 259, 476
BC4329 BC4329 Nicotinate-nucleotide adenylyltransferase (NCBI ptt file) 259, 476
BC4330 BC4330 hypothetical RNA binding protein (NCBI ptt file) 94, 259
BC4331 BC4331 Shikimate 5-dehydrogenase (NCBI ptt file) 49, 259
BC4657 BC4657 Tyrosyl-tRNA synthetase (NCBI ptt file) 236, 423
BC4911 BC4911 Arsenical pump membrane protein (NCBI ptt file) 133, 236
BC5309 BC5309 ATP synthase delta chain (NCBI ptt file) 182, 259
BC5310 BC5310 ATP synthase B chain (NCBI ptt file) 182, 259
BC5311 BC5311 ATP synthase C chain (NCBI ptt file) 182, 259
BC5312 BC5312 ATP synthase A chain (NCBI ptt file) 182, 259
BC5313 BC5313 ATP synthase protein I (NCBI ptt file) 182, 259
BC5338 BC5338 CTP synthase (NCBI ptt file) 236, 423
BC5454 BC5454 Molybdenum cofactor biosynthesis enzyme and related Fe-S oxidoreductases (NCBI ptt file) 66, 236
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for BC4326
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend