Organism : Bacillus subtilis | Module List :
BSU35370 csrA

carbon storage regulator (RefSeq)

CircVis
Functional Annotations (6)
Function System
Carbon storage regulator (could also regulate swarming and quorum sensing) cog/ cog
RNA binding go/ molecular_function
regulation of carbohydrate metabolic process go/ biological_process
mRNA metabolic process go/ biological_process
Two-component system kegg/ kegg pathway
csrA tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for BSU35370
(Mouseover regulator name to see its description)

BSU35370 is regulated by 14 influences and regulates 0 modules.
Regulators for BSU35370 csrA (14)
Regulator Module Operator
BSU12560 5 tf
BSU14480 5 tf
BSU19090 5 tf
BSU35430 5 tf
BSU07220 342 tf
BSU08250 342 tf
BSU10420 342 tf
BSU11930 342 tf
BSU12560 342 tf
BSU14480 342 tf
BSU19090 342 tf
BSU26430 342 tf
BSU29030 342 tf
BSU35430 342 tf

Warning: BSU35370 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
4972 2.20e-02 GCCGATAa
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4973 3.80e+02 tcCCtCct
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5618 1.00e-02 GCCGATAa
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5619 1.30e+02 aaAaGtaggaGgCaG
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for BSU35370

BSU35370 is enriched for 6 functions in 3 categories.
Enrichment Table (6)
Function System
Carbon storage regulator (could also regulate swarming and quorum sensing) cog/ cog
RNA binding go/ molecular_function
regulation of carbohydrate metabolic process go/ biological_process
mRNA metabolic process go/ biological_process
Two-component system kegg/ kegg pathway
csrA tigr/ tigrfam
Module neighborhood information for BSU35370

BSU35370 has total of 28 gene neighbors in modules 5, 342
Gene neighbors (28)
Gene Common Name Description Module membership
BSU02050 ybdO hypothetical protein (RefSeq) 5, 342
BSU03440 tlpC methyl-accepting chemotaxis protein (RefSeq) 5, 342
BSU10380 hemAT haem-based dioxygen sensor (RefSeq) 5, 342
BSU11570 yjbJ putative murein lytic transglycosylase (RefSeq) 5, 342
BSU11940 yjcP hypothetical protein (RefSeq) 5, 342
BSU11950 yjcQ hypothetical protein (RefSeq) 5, 342
BSU11960 yjcR putative phage-related nucleic acid binding terminase small subunit (RefSeq) 52, 342
BSU12120 yjfB hypothetical protein (RefSeq) 5, 342
BSU13420 ykoW putative sensor protein (RefSeq) 5, 342
BSU13950 mcpC methyl-accepting chemotaxis protein (RefSeq) 5, 366
BSU14010 cheV coupling protein and response regulator for CheA activity in response to attractants (chemotaxis) (RefSeq) 5, 342
BSU15960 ylqB hypothetical protein (RefSeq) 342, 392
BSU21940 degR activation of degradative enzymes (aprE, nprE, sacB) production or activity (RefSeq) 96, 342
BSU26200 yqaS putative phage-related terminase small subunit; skin element (RefSeq) 238, 342
BSU26210 yqaR hypothetical protein; skin element (RefSeq) 5, 342
BSU31250 tlpA methyl-accepting chemotaxis protein (RefSeq) 342, 392
BSU33690 yvaQ putative methyl-accepting transducer (RefSeq) 5, 96
BSU35150 yvzB putative flagellin (RefSeq) 5, 342
BSU35230 swrAA hypothetical protein (RefSeq) 5, 342
BSU35360 hag flagellin (RefSeq) 5, 392
BSU35370 csrA carbon storage regulator (RefSeq) 5, 342
BSU35380 yviF flagellar assembly protein FliW (RefSeq) 5, 342
BSU35390 yviE hypothetical protein (RefSeq) 5, 342
BSU35780 lytD exported N-acetylglucosaminidase (major autolysin) (CWBP90) (RefSeq) 5, 342
BSU35860 pgdS gamma-DL-glutamyl hydrolase (PGA depolymerase) (RefSeq) 5, 342
BSU36390 flhP putative flagellar hook-basal body protein (RefSeq) 5, 342
BSU36400 flhO putative flagellar basal-body rod protein (RefSeq) 5, 342
BSU38930 yxjJ hypothetical protein (RefSeq) 217, 342
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for BSU35370
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend