Organism : Clostridium acetobutylicum | Module List :
CAC3136 tufA

Elongation Factor Tu (Ef-Tu) (NCBI ptt file)

CircVis
Functional Annotations (8)
Function System
GTPases - translation elongation factors cog/ cog
translation elongation factor activity go/ molecular_function
GTPase activity go/ molecular_function
GTP binding go/ molecular_function
intracellular go/ cellular_component
translational elongation go/ biological_process
protein-synthesizing GTPase activity go/ molecular_function
small_GTP tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for CAC3136
(Mouseover regulator name to see its description)

CAC3136 is regulated by 24 influences and regulates 0 modules.
Regulators for CAC3136 tufA (24)
Regulator Module Operator
CAC0162 335 tf
CAC0707 335 tf
CAC0863 335 tf
CAC0933 335 tf
CAC1032 335 tf
CAC1300 335 tf
CAC1320 335 tf
CAC1832 335 tf
CAC2430 335 tf
CAC3142 335 tf
CAC3149 335 tf
CAC3199 335 tf
CAC0032 78 tf
CAC0379 78 tf
CAC0402 78 tf
CAC0849 78 tf
CAC1668 78 tf
CAC2793 78 tf
CAC2794 78 tf
CAC3214 78 tf
CAC3361 78 tf
CAC3475 78 tf
CAC3496 78 tf
CAC3677 78 tf

Warning: CAC3136 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
6810 5.40e-03 aGGAGG
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6811 3.00e+03 GGTGCG
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7322 1.20e-05 gGAGAGGatG
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7323 9.80e+02 GCCTTCACcCC
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for CAC3136

CAC3136 is enriched for 8 functions in 3 categories.
Enrichment Table (8)
Function System
GTPases - translation elongation factors cog/ cog
translation elongation factor activity go/ molecular_function
GTPase activity go/ molecular_function
GTP binding go/ molecular_function
intracellular go/ cellular_component
translational elongation go/ biological_process
protein-synthesizing GTPase activity go/ molecular_function
small_GTP tigr/ tigrfam
Module neighborhood information for CAC3136

CAC3136 has total of 43 gene neighbors in modules 78, 335
Gene neighbors (43)
Gene Common Name Description Module membership
CAC0070 CAC0070 Hypothetical protein (NCBI ptt file) 44, 78
CAC0466 CAC0466 Hypothetical protein (NCBI ptt file) 78, 177
CAC0467 CAC0467 Uncharacterized membrane protein, homolog of YDAH B.subtilis (NCBI ptt file) 78, 174
CAC0468 CAC0468 HAD superfamily hydrolase (NCBI ptt file) 78, 177
CAC0502 uvrB Excinuclease ABC subunit B (helicase subunit of the DNA excision repair complex) (NCBI ptt file) 154, 335
CAC0503 uvrA Excinuclease ABC subunit A (ATP-ase), (uvrA) (NCBI ptt file) 154, 335
CAC0710 pgk 3-phosphoglycerate kinase (NCBI ptt file) 79, 335
CAC0811 CAC0811 Hydrogenase expression-formation factor (hypD) (NCBI ptt file) 78, 125
CAC1409 CAC1409 Predicted membrane protein (NCBI ptt file) 78, 351
CAC1594 rpsD Ribosomal protein S4 (NCBI ptt file) 78, 177
CAC1735 CAC1735 Predicted kinase related to hydroxyacetone kinase, YLOV ortholog (NCBI ptt file) 36, 335
CAC1743 askA Acetate kinase (NCBI ptt file) 335, 343
CAC1748 rncS DsRNA-specific ribonuclease III (NCBI ptt file) 271, 335
CAC2036 CAC2036 Hypothetical protein (NCBI ptt file) 78, 163
CAC2347 CAC2347 Glycosyltransferase (NCBI ptt file) 78, 188
CAC2411 CAC2411 Zn-dependent protease of MPP family (NCBI ptt file) 105, 335
CAC2469 CAC2469 Lactoylglutathione lyase (fragment) (NCBI ptt file) 78, 86
CAC2470 CAC2470 Uncharacterized Zn-finger protein (NCBI ptt file) 38, 78
CAC2741 gpmA Phosphoglycerate mutase (NCBI ptt file) 78, 101
CAC2744 CAC2744 Predicted membrane protein (NCBI ptt file) 78, 338
CAC2845 prfB Protein chain release factor B (NCBI ptt file) 329, 335
CAC2862 murA UDP-N-acetylglucosamine 1-carboxyvinyltransferase (NCBI ptt file) 78, 98
CAC2864 atpC FoF1-type ATP synthase epsilon subunit (NCBI ptt file) 304, 335
CAC2866 atpG FoF1-type ATP synthase gamma subunit (NCBI ptt file) 59, 335
CAC2867 atpA FoF1-type ATP synthase alpha subunit (NCBI ptt file) 59, 335
CAC2868 atpH FoF1-type ATP synthase delta subunit (NCBI ptt file) 59, 335
CAC2869 atpF FoF1-type ATP synthase B subunit (NCBI ptt file) 79, 335
CAC3007 CAC3007 ATPase component of MDR-type ABC transporter, two ATPase domains (NCBI ptt file) 19, 78
CAC3017 CAC3017 Predicted xylanase/chitin deacetylase (NCBI ptt file) 36, 335
CAC3112 adk Adenylate kinase (NCBI ptt file) 335, 362
CAC3114 rplO Ribosomal protein L15 (NCBI ptt file) 59, 335
CAC3120 rpsN Ribosomal protein S14 (NCBI ptt file) 335, 362
CAC3136 tufA Elongation Factor Tu (Ef-Tu) (NCBI ptt file) 78, 335
CAC3138 fus Translation elongation factor EF-G (NCBI ptt file) 36, 335
CAC3142 rpoC DNA-dependent RNA polymerase beta' subunit (NCBI ptt file) 255, 335
CAC3143 rpoB DNA-dependent RNA polymerase beta subunit (NCBI ptt file) 255, 335
CAC3550 natB Na+ ABC transporter, NATB (NCBI ptt file) 78, 203
CAC3563 CAC3563 Mismatch repair protein MutS-like ATPase (NCBI ptt file) 247, 335
CAC3678 kdpD Sensor protein KdpD (ATPase containing sensor domain and histidine kinase domain) (NCBI ptt file) 22, 78
CAC3679 kdpX Uncharacterized protein of kdp operon, kdpX (NCBI ptt file) 22, 78
CAC3681 kdpB/atkB K+-transporting ATPase, b chain (NCBI ptt file) 22, 78
CAC3690 CAC3690 Uncharacterized conserved protein, related to polyketide cyclase (CypC) (NCBI ptt file) 78, 125
CAC3715 dnaC Replicative DNA helicase, DNAC (NCBI ptt file) 243, 335
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for CAC3136
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend