Organism : Halobacterium salinarum NRC-1 | Module List :
VNG0384G trpE2

anthranilate synthase subunit alpha

CircVis
Functional Annotations (9)
Function System
Anthranilate/para-aminobenzoate synthases component I cog/ cog
anthranilate synthase activity go/ molecular_function
anthranilate synthase complex go/ cellular_component
biosynthetic process go/ biological_process
4-amino-4-deoxychorismate synthase activity go/ molecular_function
Phenylalanine tyrosine and tryptophan biosynthesis kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Biosynthesis of secondary metabolites kegg/ kegg pathway
PabB-clade2 tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for VNG0384G
(Mouseover regulator name to see its description)

VNG0384G is regulated by 8 influences and regulates 0 modules.
Regulators for VNG0384G trpE2 (8)
Regulator Module Operator
VNG1377G
VNG5176C
27 combiner
VNG1886C 27 tf
VNG5142G 27 tf
VNG6389G 27 tf
VNG1377G
VNG5176C
274 combiner
VNG5142G 274 tf
VNG6143H 269 tf
VNG6389G 269 tf

Warning: VNG0384G Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 6 motifs predicted.

Motif Table (6)
Motif Id e-value Consensus Motif Logo
1031 1.00e+02 GTGtTctccGTGgACGaATTaA
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1032 7.40e+01 GcCGaaaccctCGt
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1471 3.40e+03 tTtttATA.TACT.Aatt
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1472 1.90e+04 TaTtTaTTaATCTAT
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1481 2.20e+03 AAat.CtTTgTggtT
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1482 3.70e+03 gcgtgtaCGACcccg
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for VNG0384G

VNG0384G is enriched for 9 functions in 3 categories.
Enrichment Table (9)
Function System
Anthranilate/para-aminobenzoate synthases component I cog/ cog
anthranilate synthase activity go/ molecular_function
anthranilate synthase complex go/ cellular_component
biosynthetic process go/ biological_process
4-amino-4-deoxychorismate synthase activity go/ molecular_function
Phenylalanine tyrosine and tryptophan biosynthesis kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Biosynthesis of secondary metabolites kegg/ kegg pathway
PabB-clade2 tigr/ tigrfam
Module neighborhood information for VNG0384G

VNG0384G has total of 53 gene neighbors in modules 27, 269, 274
Gene neighbors (53)
Gene Common Name Description Module membership
VNG0033H hypothetical protein VNG0033H 83, 269
VNG0061C hypothetical protein VNG0061C 251, 269
VNG0105H hypothetical protein VNG0105H 247, 269
VNG0214C hypothetical protein VNG0214C 190, 269
VNG0280H hypothetical protein VNG0280H 269
VNG0298H hypothetical protein VNG0298H 57, 269, 298
VNG0319H hypothetical protein VNG0319H 269
VNG0329G caaX zinc metalloproteinase-like protein 269
VNG0346H hypothetical protein VNG0346H 118, 269
VNG0367H hypothetical protein VNG0367H 118, 269
VNG0382G aroE hypothetical protein VNG0382G 27
VNG0384G trpE2 anthranilate synthase subunit alpha 27, 269, 274
VNG0387G ilvE1 branched-chain amino acid aminotransferase 27, 269, 274
VNG0389C hypothetical protein VNG0389C 27, 269, 274
VNG0415G purB adenylosuccinate lyase 27, 269, 274
VNG0421C hypothetical protein VNG0421C 259, 274
VNG0442G rspA hypothetical protein VNG0442G 27, 269, 274
VNG0446G gcd glucose dehydrogenase 27, 269, 274
VNG0447H hypothetical protein VNG0447H 27
VNG0448G pyrE1 orotate phosphoribosyltransferase-like protein 27
VNG0475C hypothetical protein VNG0475C 27, 274
VNG0479G yajO1 putative oxidoreductase 27, 274
VNG0812G htr18 Htr18 269
VNG0822C hypothetical protein VNG0822C 269
VNG0852C hypothetical protein VNG0852C 269
VNG0861H hypothetical protein VNG0861H 269
VNG0882Gm PHO2 p-nitrophenyl phosphatase 27, 274, 282
VNG0915G hakA atrazine chlorohydrolase 269, 284
VNG0916G ark adaptive-response sensory-kinase 27, 274, 282
VNG0917G hrg Hrg 27
VNG0919G gst hypothetical protein VNG0919G 27, 274
VNG0947G fapJ flagellar assembly protein J 27, 274
VNG0981C hypothetical protein VNG0981C 27, 274
VNG0983C hypothetical protein VNG0983C 27, 274
VNG0986H hypothetical protein VNG0986H 27, 274
VNG1353C hypothetical protein VNG1353C 269
VNG1382H hypothetical protein VNG1382H 118, 274
VNG1384H hypothetical protein VNG1384H 27, 231, 274
VNG1387H hypothetical protein VNG1387H 27, 250, 274
VNG1417H hypothetical protein VNG1417H 27, 274
VNG1420H hypothetical protein VNG1420H 274
VNG1454C hypothetical protein VNG1454C 27, 274
VNG1456H hypothetical protein VNG1456H 27
VNG1480C hypothetical protein VNG1480C 27, 274
VNG1481G sat serine acetyltransferase 190, 274
VNG1786H hypothetical protein VNG1786H 269
VNG2101H hypothetical protein VNG2101H 269
VNG2376H hypothetical protein VNG2376H 269
VNG2498H hypothetical protein VNG2498H 269
VNG6254C hypothetical protein VNG6254C 269
VNG7078 hypothetical protein VNG7078 269
VNG7082 hypothetical protein VNG7082 269
VNG7137 hypothetical protein VNG7137 269
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for VNG0384G
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend