Organism : Pseudomonas aeruginosa | Module List :
PA0448

probable transcriptional regulator (NCBI)

CircVis
Functional Annotations (3)
Function System
Transcriptional regulator cog/ cog
sequence-specific DNA binding transcription factor activity go/ molecular_function
regulation of transcription, DNA-dependent go/ biological_process
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for PA0448
(Mouseover regulator name to see its description)

PA0448 is regulated by 27 influences and regulates 16 modules.
Regulators for PA0448 (27)
Regulator Module Operator
PA0217 175 tf
PA0448 175 tf
PA0564 175 tf
PA1128 175 tf
PA1261 175 tf
PA1526 175 tf
PA2050 175 tf
PA2917 175 tf
PA3249 175 tf
PA3711 175 tf
PA3757 175 tf
PA3830 175 tf
PA4169 175 tf
PA4174 175 tf
PA5032 175 tf
PA0163 438 tf
PA0448 438 tf
PA1128 438 tf
PA1309 438 tf
PA1315 438 tf
PA1374 438 tf
PA1627 438 tf
PA1853 438 tf
PA2047 438 tf
PA3067 438 tf
PA3133 438 tf
PA5342 438 tf

Warning: PA0448 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
3178 3.80e+02 AtcGgGaaAA
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3179 6.00e+04 AAGTGTCATT
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3696 1.90e-04 T.AGaa.acgTagCtAT.aTcATg
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3697 2.40e-03 cTgGCc.aGGAAgAA
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for PA0448

PA0448 is enriched for 3 functions in 3 categories.
Enrichment Table (3)
Function System
Transcriptional regulator cog/ cog
sequence-specific DNA binding transcription factor activity go/ molecular_function
regulation of transcription, DNA-dependent go/ biological_process
Module neighborhood information for PA0448

PA0448 has total of 58 gene neighbors in modules 175, 438
Gene neighbors (58)
Gene Common Name Description Module membership
PA0142 PA0142 hydroxydechloroatrazine ethylaminohydrolase (NCBI) 175, 192
PA0163 PA0163 probable transcriptional regulator (NCBI) 344, 438
PA0223 PA0223 probable dihydrodipicolinate synthetase (NCBI) 175, 489
PA0378 mtgA monofunctional biosynthetic peptidoglycan transglycosylase (NCBI) 181, 438
PA0379 PA0379 hypothetical protein (NCBI) 387, 438
PA0448 PA0448 probable transcriptional regulator (NCBI) 175, 438
PA0550 PA0550 hypothetical protein (NCBI) 175, 420
PA0600 PA0600 probable two-component sensor (NCBI) 175, 245
PA0736 PA0736 hypothetical protein (NCBI) 372, 438
PA1113 PA1113 probable ATP-binding/permease fusion ABC transporter (NCBI) 175, 537
PA1128 PA1128 probable transcriptional regulator (NCBI) 175, 438
PA1158 PA1158 probable two-component sensor (NCBI) 175, 528
PA1227 PA1227 hypothetical protein (NCBI) 101, 438
PA1291 PA1291 hypothetical protein (NCBI) 173, 438
PA1309 PA1309 probable transcriptional regulator (NCBI) 266, 438
PA1315 PA1315 probable transcriptional regulator (NCBI) 220, 438
PA1316 PA1316 probable major facilitator superfamily (MFS) transporter (NCBI) 408, 438
PA1334 PA1334 probable oxidoreductase (NCBI) 175, 333
PA1373 fabF2 3-oxoacyl-(acyl carrier protein) synthase (NCBI) 68, 438
PA1374 PA1374 hypothetical protein (NCBI) 68, 438
PA1406 PA1406 hypothetical protein (NCBI) 175, 372
PA1425 PA1425 probable ATP-binding component of ABC transporter (NCBI) 335, 438
PA1626 PA1626 probable major facilitator superfamily (MFS) transporter (NCBI) 236, 438
PA1627 PA1627 probable transcriptional regulator (NCBI) 306, 438
PA1646 PA1646 probable chemotaxis transducer (NCBI) 175, 345
PA1652 PA1652 hypothetical protein (NCBI) 68, 438
PA1820 nhaB sodium/proton antiporter NhaB (NCBI) 175, 478
PA1825 PA1825 hypothetical protein (NCBI) 33, 175
PA1906 PA1906 hypothetical protein (NCBI) 175, 489
PA1960 PA1960 hypothetical protein (NCBI) 245, 438
PA2521 czcB Resistance-Nodulation-Cell Division (RND) divalent metal cation efflux membrane fusion protein CzcB precursor (NCBI) 175, 446
PA2563 PA2563 probable sulfate transporter (NCBI) 438, 507
PA2688 pfeA Ferric enterobactin receptor, outer membrane protein PfeA precursor (NCBI) 175, 339
PA2917 PA2917 probable transcriptional regulator (NCBI) 175, 333
PA3065 PA3065 hypothetical protein (NCBI) 324, 438
PA3066 PA3066 hypothetical protein (NCBI) 324, 438
PA3067 PA3067 probable transcriptional regulator (NCBI) 324, 438
PA3129 PA3129 hypothetical protein (NCBI) 135, 438
PA3206 PA3206 probable two-component sensor (NCBI) 175, 313
PA3312 PA3312 probable 3-hydroxyisobutyrate dehydrogenase (NCBI) 196, 438
PA3464 PA3464 hypothetical protein (NCBI) 175, 192
PA3605 PA3605 hypothetical protein (NCBI) 175, 247
PA3679 PA3679 hypothetical protein (NCBI) 335, 438
PA3680 PA3680 hypothetical protein (NCBI) 114, 438
PA3733 PA3733 hypothetical protein (NCBI) 175, 192
PA3749 PA3749 probable major facilitator superfamily (MFS) transporter (NCBI) 175, 337
PA3783 PA3783 hypothetical protein (NCBI) 175, 241
PA3830 PA3830 probable transcriptional regulator (NCBI) 33, 175
PA3927 PA3927 probable transcriptional regulator (NCBI) 33, 175
PA3960 PA3960 hypothetical protein (NCBI) 175, 408
PA4082 cupB5 adhesive protein CupB5 (NCBI) 175, 192
PA4146 PA4146 hypothetical protein (NCBI) 175, 536
PA4167 PA4167 probable oxidoreductase (NCBI) 175, 266
PA4609 radA DNA repair protein RadA (NCBI) 306, 438
PA4804 PA4804 probable amino acid permease (NCBI) 175, 267
PA5292 pchP phosphorylcholine phosphatase (NCBI) 175, 292
PA5412 PA5412 hypothetical protein (NCBI) 359, 438
PA5442 PA5442 hypothetical protein (NCBI) 266, 438
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for PA0448
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend