Organism : Pseudomonas aeruginosa | Module List :
PA1046

hypothetical protein (NCBI)

CircVis
Functional Annotations (3)
Function System
catalytic activity go/ molecular_function
carbohydrate metabolic process go/ biological_process
cation binding go/ molecular_function
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for PA1046
(Mouseover regulator name to see its description)

PA1046 is regulated by 25 influences and regulates 0 modules.
Regulators for PA1046 (25)
Regulator Module Operator
PA0037 375 tf
PA0294 375 tf
PA0306 375 tf
PA0455 375 tf
PA1884 375 tf
PA1998 375 tf
PA2115 375 tf
PA2121 375 tf
PA2547 375 tf
PA3477 375 tf
PA3778 375 tf
PA3898 375 tf
PA5253 375 tf
PA0701 241 tf
PA1153 241 tf
PA1223 241 tf
PA1261 241 tf
PA2005 241 tf
PA2050 241 tf
PA3381 241 tf
PA3433 241 tf
PA4341 241 tf
PA4914 241 tf
PA5032 241 tf
PA5431 241 tf

Warning: PA1046 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
3310 3.80e+04 CGgaTaccCCAGatactC.CG
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3311 2.80e+04 AcGccT.TCctCacTG
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3574 3.10e-02 CatGAAGTcaatGGaCAATcaaGA
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3575 6.60e-02 tc.TttctGTgCgGagaAacctgc
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for PA1046

PA1046 is enriched for 3 functions in 2 categories.
Enrichment Table (3)
Function System
catalytic activity go/ molecular_function
carbohydrate metabolic process go/ biological_process
cation binding go/ molecular_function
Module neighborhood information for PA1046

PA1046 has total of 53 gene neighbors in modules 241, 375
Gene neighbors (53)
Gene Common Name Description Module membership
PA0010 tag DNA-3-methyladenine glycosidase I (NCBI) 65, 375
PA0013 PA0013 hypothetical protein (NCBI) 335, 375
PA0037 trpI transcriptional regulator TrpI (NCBI) 375, 377
PA0294 aguR transcriptional regulator AguR (NCBI) 20, 375
PA0305 PA0305 hypothetical protein (NCBI) 375, 380
PA0306 PA0306 probable transcriptional regulator (NCBI) 295, 375
PA0383 PA0383 hypothetical protein (NCBI) 375, 377
PA0544 PA0544 hypothetical protein (NCBI) 313, 375
PA0545 PA0545 hypothetical protein (NCBI) 162, 375
PA0560 PA0560 hypothetical protein (NCBI) 229, 375
PA0602 PA0602 probable binding protein component of ABC transporter (NCBI) 31, 241
PA0703 PA0703 probable major facilitator superfamily (MFS) transporter (NCBI) 283, 375
PA0714 PA0714 hypothetical protein (NCBI) 241, 311
PA0847 PA0847 hypothetical protein (NCBI) 241, 523
PA1044 PA1044 hypothetical protein (NCBI) 101, 375
PA1046 PA1046 hypothetical protein (NCBI) 241, 375
PA1110 PA1110 hypothetical protein (NCBI) 101, 375
PA1133 PA1133 hypothetical protein (NCBI) 171, 241
PA1153 PA1153 hypothetical protein (NCBI) 104, 241
PA1234 PA1234 hypothetical protein (NCBI) 20, 241
PA1488 PA1488 hypothetical protein (NCBI) 295, 375
PA1489 PA1489 hypothetical protein (NCBI) 295, 375
PA1636 kdpD two-component sensor KdpD (NCBI) 241, 313
PA1637 kdpE two-component response regulator KdpE (NCBI) 241, 313
PA1831 PA1831 hypothetical protein (NCBI) 283, 375
PA1844 PA1844 hypothetical protein (NCBI) 235, 375
PA1884 PA1884 probable transcriptional regulator (NCBI) 335, 375
PA2005 PA2005 probable transcriptional regulator (NCBI) 241, 489
PA2596 PA2596 hypothetical protein (NCBI) 241, 497
PA2628 PA2628 hypothetical protein (NCBI) 20, 375
PA2661 PA2661 hypothetical protein (NCBI) 375, 478
PA3016 PA3016 hypothetical protein (NCBI) 48, 375
PA3358 PA3358 hypothetical protein (NCBI) 171, 241
PA3403 PA3403 hypothetical protein (NCBI) 372, 375
PA3532 PA3532 hypothetical protein (NCBI) 375, 377
PA3669 PA3669 hypothetical protein (NCBI) 104, 241
PA3670 PA3670 hypothetical protein (NCBI) 104, 241
PA3783 PA3783 hypothetical protein (NCBI) 175, 241
PA3826 PA3826 hypothetical protein (NCBI) 266, 375
PA3886 PA3886 hypothetical protein (NCBI) 187, 241
PA4093 PA4093 hypothetical protein (NCBI) 241, 489
PA4347 PA4347 hypothetical protein (NCBI) 241, 253
PA4921 PA4921 hypothetical protein (NCBI) 206, 241
PA4999 waaL O-antigen ligase, WaaL (NCBI) 231, 375
PA5089 PA5089 hypothetical protein (NCBI) 241, 473
PA5121 PA5121 hypothetical protein (NCBI) 40, 375
PA5283 PA5283 probable transcriptional regulator (NCBI) 241, 434
PA5444 PA5444 hypothetical protein (NCBI) 206, 241
PA5457 PA5457 hypothetical protein (NCBI) 20, 375
PA5468 PA5468 probable citrate transporter (NCBI) 344, 375
PA5469 PA5469 hypothetical protein (NCBI) 344, 375
PA5514 poxB probable beta-lactamase (NCBI) 241, 469
PA5518 PA5518 probable potassium efflux transporter (NCBI) 40, 375
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for PA1046
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend