Organism : Pseudomonas aeruginosa | Module List :
PA2601

probable transcriptional regulator (NCBI)

CircVis
Functional Annotations (3)
Function System
Transcriptional regulator cog/ cog
sequence-specific DNA binding transcription factor activity go/ molecular_function
regulation of transcription, DNA-dependent go/ biological_process
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for PA2601
(Mouseover regulator name to see its description)

PA2601 is regulated by 28 influences and regulates 10 modules.
Regulators for PA2601 (28)
Regulator Module Operator
PA0289 469 tf
PA0939 469 tf
PA0942 469 tf
PA1290 469 tf
PA1754 469 tf
PA1760 469 tf
PA2601 469 tf
PA2718 469 tf
PA2885 469 tf
PA3322 469 tf
PA4203 469 tf
PA4270 469 tf
PA4745 469 tf
PA5105 469 tf
PA5261 469 tf
PA0032 346 tf
PA0056 346 tf
PA0528 346 tf
PA1328 346 tf
PA2050 346 tf
PA2432 346 tf
PA2469 346 tf
PA2601 346 tf
PA3249 346 tf
PA3630 346 tf
PA3776 346 tf
PA4203 346 tf
PA5032 346 tf

Warning: PA2601 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
3518 2.20e-11 Ttcatataagtaaattt..aaaAa
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3519 6.00e-06 tTtt.tTtaTgcaat
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3754 1.70e-16 actgT.gcAAAcatttTttTAttc
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3755 1.10e-04 AAtgcaTtT.gcATcgAtactAac
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for PA2601

PA2601 is enriched for 3 functions in 3 categories.
Enrichment Table (3)
Function System
Transcriptional regulator cog/ cog
sequence-specific DNA binding transcription factor activity go/ molecular_function
regulation of transcription, DNA-dependent go/ biological_process
Module neighborhood information for PA2601

PA2601 has total of 51 gene neighbors in modules 346, 469
Gene neighbors (51)
Gene Common Name Description Module membership
PA0032 PA0032 probable transcriptional regulator (NCBI) 346, 542
PA0056 PA0056 probable transcriptional regulator (NCBI) 346, 542
PA0289 gpuR transcriptional activator GpuR (NCBI) 443, 469
PA0417 chpE probable chemotaxis protein (NCBI) 283, 346
PA0528 PA0528 probable transcriptional regulator (NCBI) 346, 537
PA0939 PA0939 hypothetical protein (NCBI) 243, 469
PA0940 PA0940 hypothetical protein (NCBI) 243, 469
PA0941 PA0941 hypothetical protein (NCBI) 243, 469
PA0942 PA0942 probable transcriptional regulator (NCBI) 243, 469
PA0959 PA0959 hypothetical protein (NCBI) 205, 469
PA0960 PA0960 hypothetical protein (NCBI) 205, 469
PA0990 PA0990 hypothetical protein (NCBI) 450, 469
PA1066 PA1066 short chain dehydrogenase (NCBI) 68, 346
PA1239 PA1239 hypothetical protein (NCBI) 346, 537
PA1240 PA1240 probable enoyl-CoA hydratase/isomerase (NCBI) 173, 346
PA1290 PA1290 probable transcriptional regulator (NCBI) 469, 546
PA1575 PA1575 hypothetical protein (NCBI) 63, 469
PA1957 PA1957 hypothetical protein (NCBI) 313, 469
PA1994 PA1994 hypothetical protein (NCBI) 292, 469
PA1995 PA1995 hypothetical protein (NCBI) 457, 469
PA1997 PA1997 probable AMP-binding enzyme (NCBI) 294, 469
PA2245 pslO hypothetical protein (NCBI) 24, 346
PA2246 bkdR transcriptional regulator BkdR (NCBI) 338, 346
PA2268 PA2268 hypothetical protein (NCBI) 346, 519
PA2307 PA2307 probable permease of ABC transporter (NCBI) 22, 346
PA2308 PA2308 probable ATP-binding component of ABC transporter (NCBI) 22, 346
PA2374 PA2374 hypothetical protein (NCBI) 290, 346
PA2432 PA2432 probable transcriptional regulator (NCBI) 196, 346
PA2469 PA2469 probable transcriptional regulator (NCBI) 22, 346
PA2590 PA2590 hypothetical protein (NCBI) 173, 346
PA2598 PA2598 hypothetical protein (NCBI) 301, 346
PA2601 PA2601 probable transcriptional regulator (NCBI) 346, 469
PA2602 PA2602 hypothetical protein (NCBI) 346, 469
PA2603 PA2603 probable thiosulfate sulfurtransferase (NCBI) 346, 469
PA2723 PA2723 hypothetical protein (NCBI) 203, 469
PA2814 PA2814 hypothetical protein (NCBI) 136, 469
PA2816 PA2816 hypothetical protein (NCBI) 328, 469
PA2885 PA2885 probable transcriptional regulator (NCBI) 469, 541
PA3776 PA3776 probable transcriptional regulator (NCBI) 346, 537
PA3787 PA3787 hypothetical protein (NCBI) 432, 469
PA4201 ddlA D-alanine-D-alanine ligase A (NCBI) 443, 469
PA4202 PA4202 hypothetical protein (NCBI) 346, 469
PA4203 PA4203 probable transcriptional regulator (NCBI) 346, 469
PA4833 PA4833 hypothetical protein (NCBI) 361, 469
PA4983 PA4983 probable two-component response regulator (NCBI) 245, 469
PA5115 PA5115 hypothetical protein (NCBI) 469, 483
PA5422 PA5422 hypothetical protein (NCBI) 397, 469
PA5432 PA5432 probable acetyltransferase (NCBI) 469, 497
PA5439 PA5439 probable glucose-6-phosphate dehydrogenase (NCBI) 190, 469
PA5447 wbpZ glycosyltransferase WbpZ (NCBI) 3, 469
PA5514 poxB probable beta-lactamase (NCBI) 241, 469
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for PA2601
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend