Organism : Pseudomonas aeruginosa | Module List :
PA4853 fis

DNA-binding protein Fis (NCBI)

CircVis
Functional Annotations (3)
Function System
Factor for inversion stimulation Fis, transcriptional activator cog/ cog
sequence-specific DNA binding transcription factor activity go/ molecular_function
regulation of transcription, DNA-dependent go/ biological_process
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for PA4853
(Mouseover regulator name to see its description)

PA4853 is regulated by 30 influences and regulates 105 modules.
Regulators for PA4853 fis (30)
Regulator Module Operator
PA0155 308 tf
PA0179 308 tf
PA0576 308 tf
PA0815 308 tf
PA0890 308 tf
PA1504 308 tf
PA1759 308 tf
PA2281 308 tf
PA2622 308 tf
PA2957 308 tf
PA3804 308 tf
PA3965 308 tf
PA4052 308 tf
PA4451 308 tf
PA4745 308 tf
PA4853 308 tf
PA4890 308 tf
PA5337 308 tf
PA5550 308 tf
PA0125 474 tf
PA1422 474 tf
PA2957 474 tf
PA3604 474 tf
PA3622 474 tf
PA4275 474 tf
PA4279 474 tf
PA4451 474 tf
PA4745 474 tf
PA4853 474 tf
PA5239 474 tf
Regulated by PA4853 (105)
Module Residual Genes
4 0.56 17
13 0.42 12
19 0.41 12
21 0.36 12
28 0.53 23
38 0.46 17
40 0.52 21
48 0.50 24
56 0.39 11
59 0.54 21
65 0.50 26
72 0.56 19
78 0.54 18
86 0.59 20
88 0.56 24
90 0.40 14
99 0.55 19
100 0.59 23
102 0.36 11
111 0.45 11
114 0.48 17
122 0.45 14
126 0.56 17
135 0.49 21
143 0.55 21
162 0.53 23
165 0.56 25
185 0.54 19
186 0.42 13
193 0.49 22
194 0.41 17
195 0.57 21
198 0.50 18
204 0.47 18
207 0.45 18
210 0.38 13
217 0.32 11
223 0.42 12
228 0.51 17
230 0.43 16
232 0.46 13
239 0.53 25
247 0.49 26
249 0.52 13
255 0.51 18
258 0.53 17
259 0.42 13
262 0.40 11
263 0.54 17
265 0.49 21
269 0.45 16
271 0.52 17
272 0.41 13
274 0.51 18
276 0.53 12
280 0.46 24
281 0.51 14
286 0.52 15
299 0.50 26
308 0.41 12
314 0.44 14
315 0.59 21
318 0.41 19
322 0.45 19
332 0.52 19
352 0.43 9
354 0.47 15
360 0.41 13
371 0.58 22
374 0.55 31
398 0.48 18
399 0.49 18
404 0.42 12
405 0.52 20
416 0.42 15
417 0.27 12
419 0.32 11
422 0.52 21
424 0.43 13
429 0.38 13
433 0.44 16
440 0.43 14
442 0.51 21
451 0.51 15
454 0.34 11
465 0.41 12
474 0.45 15
477 0.58 23
479 0.44 17
490 0.52 19
491 0.54 28
501 0.51 17
504 0.39 11
506 0.32 9
510 0.37 10
517 0.52 25
520 0.45 17
521 0.48 26
533 0.38 14
535 0.41 12
539 0.28 12
541 0.55 23
543 0.45 12
549 0.43 9
555 0.36 11
Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
3442 6.90e-03 GgTAtAaTgccccgCtTt
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3443 1.00e+04 CAgcaAgccAC
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3764 2.70e-01 gaaAAgcct.c.Att
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3765 4.20e+02 aCcCt.tataAtgCg
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for PA4853

PA4853 is enriched for 3 functions in 3 categories.
Module neighborhood information for PA4853

PA4853 has total of 26 gene neighbors in modules 308, 474
Gene neighbors (26)
Gene Common Name Description Module membership
PA0330 rpiA ribose-5-phosphate isomerase A (NCBI) 308, 440
PA0577 dnaG DNA primase (NCBI) 404, 474
PA0578 PA0578 hypothetical protein (NCBI) 404, 474
PA0592 ksgA dimethyladenosine transferase (NCBI) 308, 520
PA0654 speD S-adenosylmethionine decarboxylase proenzyme (NCBI) 272, 308
PA0945 purM phosphoribosylaminoimidazole synthetase (NCBI) 272, 308
PA1192 PA1192 hypothetical protein (NCBI) 354, 474
PA3701 prfB peptide chain release factor H (NCBI) 308, 544
PA3806 PA3806 hypothetical protein (NCBI) 308, 395
PA3979 PA3979 hypothetical protein (NCBI) 232, 474
PA4278 PA4278 hypothetical protein (NCBI) 126, 474
PA4279 PA4279 hypothetical protein (NCBI) 126, 474
PA4280 birA biotin--protein ligase (NCBI) 299, 474
PA4455 PA4455 probable permease of ABC transporter (NCBI) 474, 517
PA4456 PA4456 probable ATP-binding component of ABC transporter (NCBI) 178, 474
PA4481 mreB rod shape-determining protein MreB (NCBI) 56, 474
PA4748 tpiA triosephosphate isomerase (NCBI) 404, 474
PA4768 smpB SsrA-binding protein (NCBI) 255, 474
PA4852 PA4852 hypothetical protein (NCBI) 424, 474
PA4853 fis DNA-binding protein Fis (NCBI) 308, 474
PA4854 purH bifunctionalphosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (NCBI) 90, 308
PA5298 PA5298 xanthine phosphoribosyltransferase (NCBI) 308, 549
PA5425 purK phosphoribosylaminoimidazole carboxylase (NCBI) 90, 308
PA5426 purE phosphoribosylaminoimidazole carboxylase, catalytic subunit (NCBI) 90, 308
PA5503 PA5503 probable ATP-binding component of ABC transporter (NCBI) 56, 474
PA5570 rpmH 50S ribosomal protein L34 (NCBI) 282, 308
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for PA4853
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend