Organism : Pseudomonas aeruginosa | Module List :
PA5224 pepP

aminopeptidase P (NCBI)

CircVis
Functional Annotations (7)
Function System
Xaa-Pro aminopeptidase cog/ cog
transporter activity go/ molecular_function
proteolysis go/ biological_process
transport go/ biological_process
metalloexopeptidase activity go/ molecular_function
X-Pro aminopeptidase activity go/ molecular_function
manganese ion binding go/ molecular_function
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for PA5224
(Mouseover regulator name to see its description)

PA5224 is regulated by 30 influences and regulates 0 modules.
Regulators for PA5224 pepP (30)
Regulator Module Operator
PA0393 42 tf
PA0893 42 tf
PA2020 42 tf
PA2054 42 tf
PA3002 42 tf
PA3699 42 tf
PA3965 42 tf
PA4052 42 tf
PA4057 42 tf
PA4080 42 tf
PA4182 42 tf
PA4269 42 tf
PA4270 42 tf
PA4493 42 tf
PA4755 42 tf
PA5253 42 tf
PA5324 42 tf
PA5344 42 tf
PA0167 540 tf
PA0393 540 tf
PA1945 540 tf
PA2376 540 tf
PA3804 540 tf
PA4147 540 tf
PA4269 540 tf
PA4493 540 tf
PA4784 540 tf
PA5166 540 tf
PA5253 540 tf
PA5344 540 tf

Warning: PA5224 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
2916 2.00e+03 gctTGCCggcg
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2917 6.40e+02 tc.c.GCccccttCGcggcaa
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3892 4.30e+04 TATAaAGA
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3893 4.90e+04 GcatgGcGccAtcc.gccgcT
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for PA5224

PA5224 is enriched for 7 functions in 3 categories.
Enrichment Table (7)
Function System
Xaa-Pro aminopeptidase cog/ cog
transporter activity go/ molecular_function
proteolysis go/ biological_process
transport go/ biological_process
metalloexopeptidase activity go/ molecular_function
X-Pro aminopeptidase activity go/ molecular_function
manganese ion binding go/ molecular_function
Module neighborhood information for PA5224

PA5224 has total of 39 gene neighbors in modules 42, 540
Gene neighbors (39)
Gene Common Name Description Module membership
PA0167 PA0167 probable transcriptional regulator (NCBI) 316, 540
PA0353 ilvD dihydroxy-acid dehydratase (NCBI) 42, 420
PA1293 PA1293 hypothetical protein (NCBI) 389, 540
PA1615 PA1615 probable lipase (NCBI) 42, 198
PA2529 PA2529 hypothetical protein (NCBI) 49, 540
PA2530 PA2530 hypothetical protein (NCBI) 378, 540
PA3028 moeA2 molybdenum cofactor biosynthesis protein A2 (NCBI) 420, 540
PA3029 moaB2 molybdopterin biosynthetic protein B2 (NCBI) 7, 540
PA3301 PA3301 hypothetical protein (NCBI) 42, 263
PA3302 PA3302 hypothetical protein (NCBI) 42, 436
PA3481 PA3481 hypothetical protein (NCBI) 420, 540
PA3831 pepA leucyl aminopeptidase (NCBI) 264, 540
PA3832 holC DNA polymerase III subunit chi (NCBI) 37, 540
PA3997 lipB lipoyltransferase (NCBI) 42, 262
PA4163 PA4163 amidase (NCBI) 42, 342
PA4164 PA4164 hypothetical protein (NCBI) 42, 342
PA4176 ppiC2 peptidyl-prolyl cis-trans isomerase C2 (NCBI) 42, 342
PA4180 PA4180 hypothetical protein (NCBI) 42, 342
PA4182 PA4182 hypothetical protein (NCBI) 42, 268
PA4423 PA4423 hypothetical protein (NCBI) 42, 263
PA4576 PA4576 probable ATP-dependent protease (NCBI) 191, 540
PA4595 PA4595 probable ATP-binding component of ABC transporter (NCBI) 42, 238
PA4808 selA selenocysteine synthase (NCBI) 381, 540
PA4863 PA4863 hypothetical protein (NCBI) 49, 540
PA4865 ureA urease gamma subunit (NCBI) 380, 540
PA4866 PA4866 hypothetical protein (NCBI) 380, 540
PA4872 PA4872 hypothetical protein (NCBI) 51, 540
PA4907 PA4907 probable short-chain dehydrogenase (NCBI) 42, 420
PA4974 PA4974 probable outer membrane protein precursor (NCBI) 435, 540
PA5166 PA5166 probable two-component response regulator (NCBI) 130, 540
PA5214 gcvH1 glycine cleavage system protein H1 (NCBI) 42, 420
PA5224 pepP aminopeptidase P (NCBI) 42, 540
PA5277 lysA diaminopimelate decarboxylase (NCBI) 42, 228
PA5278 dapF diaminopimelate epimerase (NCBI) 42, 198
PA5281 PA5281 probable hydrolase (NCBI) 42, 253
PA5340 PA5340 hypothetical protein (NCBI) 42, 160
PA5344 PA5344 probable transcriptional regulator (NCBI) 123, 540
PA5455 PA5455 hypothetical protein (NCBI) 215, 540
PA5459 PA5459 hypothetical protein (NCBI) 215, 540
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for PA5224
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend