New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_014034	= 744791	76 (0.170)	73 (0.160)	5/198	39.0	0.40	intergenic (+176/+36)	idi2/bchO	isopentenyl‑diphosphate delta‑isomerase/magnesium‑chelatase BchO
	?	NC_014034	= 2330174	143 (0.310)					intergenic (+149/+163)	RCAP_rcc02161/mrcA	hypothetical protein/penicillin‑binding protein 1A
Rejected: Position hash score below cutoff.

CAAGATGAAATGAATGACTTTCCGTCTGCGGCGGTACAGCCTGTCCCACCCCTGCTTTTCCTTGGCAAAATACGCCGGGGGGTGCGGGGGGCGGAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATCCGCGGCAACCTCGTGGATCAG  >  NC_014034/744692‑744889                                                                                                    |                                                                                                   cAAGATGAAATGAATGACTTTCCGTCTGCGGCGGTACAGCCTGTCCCACCCCTGCTTTTCCTTGGCACAATACGCCGGGGGGTGCGGGGGGCGGAGccccc                                                                                                   <  1:8205333/101‑1 (MQ=255) cAAGATGAAATGAATGACTTTCCGTCTGCGGCGGTACAGCCTGTCCCACCCCTGCTTTTCCTTGGCAAAATACGCCGGGGGGTGCGGGGGGCGGAGccccc                                                                                                   <  1:5853030/101‑1 (MQ=255)  aaGATGAAATGAATGACTTTCCGTCTGCGGCGGTACAGCCTGTCCCACCCCTGCTTTTCCTTGGCAAAATACGCCGGGGGGTGCGGGGGGCGGAGcccccc                                                                                                  <  2:7396048/101‑1 (MQ=255)       gaaatgaaTGACTTTCCGTCTGCGGCGGTACAGCCTGTCCCACCCCTGCTTTTCCTTGGCAAAATACGCCGGGGGGTGCGGGGGGCGGAGCCCCCCGCggg                                                                                             >  2:2085745/1‑101 (MQ=255)           tgaatgaCTTTCCGTCTGCGGCGGTACAGCCTGTCCCACCCCTGCTTTTCCTTGGCAAAATACGCCGGGGGGTGCGGGGGGCGGAGCCCCCCGCGGGGCGc                                                                                         >  2:6380914/1‑101 (MQ=255)             aatgaCTTTCCGTCTGCGGCGGTACAGCCTGTCCCACCCCTGCTTTTCCTTGGCAAAATACGCCGGGGGGTGCGGGGGGCGGAGCCCCCCGCGGGGCGccc                                                                                       >  2:4460499/1‑100 (MQ=255)                gaCTTTCCGTCTGCGGCGGTACAGCCTGTCCCACCCCTGCTTTTCCTTGGCAAAATACGCCGGGGGGTGCGGGGGGCGGAGCCCCCCGCGGGTCGCCGCgg                                                                                    >  2:7407418/1‑101 (MQ=255)                    ttCCGTCTGCGGCGGTACAGCCTGTCCCACCCCTGCTTTTCCTTGGCAAAATACGCCGGGGGGTGCGGGGGGCGGAGCCCCCCGCGGGGCGCCTCGGGGCt                                                                                >  2:1514966/1‑101 (MQ=255)                     tCCGTCTGCGGCGGTACAGCCTGTCCCACCCCTGCTTTTCCTTGGCAAAATACGCCGGGGGGTGCGGGGGGCGGAGCCCCCCGCGGGTCGCCTCCGGcccg                                                                               >  1:6773094/1‑99 (MQ=255)                      gaccttGCGGCGGTACAGCCTGTCCCACCCCTGCTTTTCCTTGGCAAAATACGCCGGGGGGTGCGGGGGGCGGAGCCCCCCGCGGGTCGCCTCGGGCCTGc                                                                              >  2:4667884/6‑101 (MQ=255)                        gTCTGCGGCGGTACAGCCTGTCCCACCCCTGCTTTTCCTTGGCAAAATACGCCGGGGGGTGCGGGGGGCGGAGCCCCCCGCGGGGCGCCTCGGGCCTGccc                                                                            >  1:8112208/1‑101 (MQ=255)                         tCTGCGGCGGTACAGCCTGTCCCACCCCTGCTTTTCCTTGGCAAAATACGCCGGGGGGTGCGGGGGGCGGAGCCCCCCGCGGGTCGCCCAGGGCCTGCCCg                                                                           >  2:5341697/1‑101 (MQ=255)                          cTGCGGCGGTACAGCCTGTCCCACCCCTGCTTTTCCTTGGCAAAATACGCCGGGGGGTGCGGGGGGCGGAGCCCCCCGCGGGTCGCCTCCGGCCCGcccca                                                                          >  2:3060257/1‑99 (MQ=255)                              ggcggTACAGCCTGTCCCACCCCTGCTTTTCCTTGGCAAAATACGCCGGGGGGTGCGGGGGGCGGAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCCAGGCg                                                                      >  2:5128411/1‑101 (MQ=255)                                                      gCTTTTCCTTGGCAAAATACGCCGGGGGGTGCGGGGGGCGGAGCCCCCCGCGGGTCGCCTCGGGCCCGCCCGAGGCGAAATCTCCCCCCCCGAGCCACTcc                                              >  2:1168703/1‑101 (MQ=18)                                                          ttCCTTGGCAAAATACGCCGGGGGGTGCGGGGGGCGGAGCCCCCCGCGGGGCGCCTCGGGCCTGCCCGGGGCGAAAACTCACCCCTCGAGCCAGGCCAGaa                                          >  2:2111519/1‑101 (MQ=21)                                                            ccTTGGCAAAATACGCCGGGGGGTGCGGGGGGCGGAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTTACCCCTCGAGCCGGCCCAggaca                                        >  2:8928160/1‑97 (MQ=18)                                                            ccTTGGCAAAATACGCCGGGGGGTGCGGGGGGCGGAGCCCCCCGCGGGTCGCCTCGGGCCCGCCCGAGGCGAAATCTCACCCCCCGAGCCAGTCCAGAatc                                        >  1:8984600/1‑101 (MQ=255)                                                            ccTTGGCAAAATACGCCGGGGGGTGCGGGGGGCGGAGCCCCCCGCGGGGCGCCTCGGGCCTGGCCCCGGCGGAATCCCACCCCTCGAGCCAGTCCAGAatc                                        >  1:2020886/1‑101 (MQ=21)                                                            ccTTGGCAAAATACGCCGGGGGGTGCGGGGGGCGGAGCCCCCCGCGGGGCGCCCCCGGCCCGCCCGGGGCCAAATCCCACCCCCCGCGCCCGCCCCGAatc                                        >  1:361955/1‑101 (MQ=14)                                                               tGGCAAAATACGCCGGGGGGTGCGGGGGGCGGAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCCCCCCCCGCGCCCGTCCAGAatcacc                                     >  2:7402720/1‑99 (MQ=21)                                                                  cAAAATACGCCGGGGGGTGCGGGGGGCGGAGCCCCCCGCGGGTCGCCTCGGGCCCGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAAACATCCCt                                  >  1:5836933/1‑101 (MQ=255)                                                                    aaaTACGCCGGGGGGTGCGGGGGGCGGAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCCCGAGCCAGTCCAGAATCATCCCTGa                                >  2:3958028/1‑101 (MQ=255)                                                                     aaTACGCCGGGGGGTGCGGGGGGCGGAGCCCCCCGTGGGGCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGaa                               >  1:8253354/1‑101 (MQ=255)                                                                      aTACGCCGGGGGGTGCGGGGGGCGGAGCCCCCCGCGGGTCGCCTCCGGCCCGCCCGAGGCGAAATCTCACCCCTCGAGCCCGTCCCGGATCATCCCTGgaa                              >  2:5642983/1‑101 (MQ=25)                                                                        aCGCCGGGGGGTGCGGGGGGCGGAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGc                            >  1:3791112/1‑101 (MQ=255)                                                                        aCGCCGGGGGGTGCGGGGGGCGGAGCCCCCCGCGGGGCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCCGAAAGc                            >  2:5142798/1‑101 (MQ=255)                                                                          gCCGGGGGGTGCGGGGGGCGGAGCCCCCCGCGGGTCGCCCCGGGGCGGCCCCAGGGGAAAACTCACCCCCCGAGCCAGTTCAGCATCAGCCCCGaaattcc                          >  2:7535877/1‑97 (MQ=17)                                                                             ggggggTGCGGGGGGCGGAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCCGTCCAGAATCATCCCTGAAAGCCCATc                       >  2:8124001/1‑101 (MQ=255)                                                                             ggggggTGCGGGGGGCGGAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCCAGCCAGTCCAGAATCATCCCTGAAAGCCCATc                       >  1:8565512/1‑101 (MQ=255)                                                                              gggggTGCGGGGGGCGGAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCCGCCCAGAATCATCCCTGAAAGCCCCTcc                      >  2:8146669/1‑101 (MQ=255)                                                                              gggggTGCGGGGGGCGGAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCCAGCCAGTCCAGAATCATCCCTGAAAGccccccc                      >  2:8528997/1‑97 (MQ=255)                                                                              gggggTGCGGGGGGCGGAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCCCGAGCCCGTCCAGAATCATCCCTGAAAGCCCATcc                      >  1:3322825/1‑101 (MQ=255)                                                                              gggggTGCGGGGGGCGGAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCCCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATcc                      >  2:3492123/1‑101 (MQ=255)                                                                               ggggTGCGGGGGGCGGAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATCcg                     >  1:2380372/1‑101 (MQ=255)                                                                                gggTGCGGGGGGCGGAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATcccc                    >  2:2576048/1‑99 (MQ=255)                                                                                gggTGCGGGGGGCGGAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATCcgc                    >  1:8635070/1‑101 (MQ=255)                                                                                gggTGCGGGGGGCGGAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATCcgc                    >  1:2476275/1‑101 (MQ=255)                                                                                gggTGCGGGGGGCGGAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATCcgc                    >  2:5717759/1‑101 (MQ=255)                                                                                gggTGCGGGGGGCGGAGCCCCCCGCGGGTCGCCTCGGGCCCGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATCcgc                    >  2:2655454/1‑101 (MQ=255)                                                                                  gTGCGGGGGGCGGAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATCCGCgg                  >  2:2238676/1‑101 (MQ=255)                                                                                  gTGCGGGGGGCGGAGCCCCCCGCGGGGCGCCTCGGGCCTGCCCGAGGCGAAATCTCAGCCCTCCAGCCAGTCCAGAATCATCCCTGAAAGCCCACCCGCgg                  >  1:2538715/1‑101 (MQ=38)                                                                                  gTGCGGGGGGCGGAGCCCCCCGCGGGCCGCCTCGGGCCTGCCCGAGGCGCAATCTCACCCCTCGCGCCAGCCCACGATGCTCCCTGAAAGCCCATCcccgc                  >  2:9173179/1‑100 (MQ=25)                                                                                  gTGCAGGGGGCGGAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATCCGCgg                  >  2:6362770/1‑101 (MQ=255)                                                                                   tGCGGGGGGCGGAGCCCCCCGCGGGTCGCCTCGGGGCTGCCCGGGGCGAAATCTCACCCCTCGAGCCCGTCCAGAATCATCCCTGAAAGGCCATCCGCGGc                 >  2:1046761/1‑101 (MQ=37)                                                                                   tGCGGGGGGCGGAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCCGAAAGCCCATCCGCGGc                 >  1:2276904/1‑101 (MQ=255)                                                                                    gCGGGGGGCGGAGCCCCCCGCGGGTCGGCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATCCGCGGCa                >  2:8137368/1‑101 (MQ=255)                                                                                    gCGGGGGGCGGAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATCCGCGGCa                >  1:9123440/1‑101 (MQ=255)                                                                                    gCGGGGGGCGGAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATCCGCCGCa                >  2:2022012/1‑101 (MQ=255)                                                                                    gCGGGGGGCGGAGCCCCCCGCGGGGCGCCTCGGGCCTGCCCGCGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATCCGCGGCa                >  1:754691/1‑101 (MQ=255)                                                                                      ggggggCGGAGCCCCCCGCGGGTCGCCTCGGGGCTGCCCGAGGCGAAATCTCACCCCTCGCGCGGGTCCAGAACCATCCCTGAAAGCCCATCCGGGGCAAc              >  2:5564567/1‑101 (MQ=25)                                                                                      ggggggCGGAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATCCGCGGCAAc              >  1:2123448/1‑101 (MQ=255)                                                                                      ggggggCGGAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATCCGCGGCAAc              >  2:5147446/1‑101 (MQ=255)                                                                                      ggggggCGGAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATCCGCGGCAAc              >  1:1275112/1‑101 (MQ=255)                                                                                      ggggggCGGAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATCCGCGGCAAc              >  1:7116802/1‑101 (MQ=255)                                                                                      ggggggCGGAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATCCGCGGCAAc              >  1:7795537/1‑101 (MQ=255)                                                                                      ggggggCGGAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATCCGCGGCAAc              >  2:1092770/1‑101 (MQ=255)                                                                                      ggggggCGGAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAAACCCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATCCGCGGCCAc              >  1:7676787/1‑101 (MQ=255)                                                                                       gggggCGGAGCCCCCCGCGGGTCGCCTCGGGCCTGACCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATCCGCGGCAAcc             >  1:8253842/1‑101 (MQ=255)                                                                                          ggCGGAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATCCGCGGCAACCTCg          >  1:4215601/1‑101 (MQ=255)                                                                                          ggCGGAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATCCGCGGCAACCTCg          >  2:8311122/1‑101 (MQ=255)                                                                                           gCGGAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATCCGCGGCAACCTCGt         >  2:2292803/1‑101 (MQ=255)                                                                                           gCGGAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATCCGCGGCAACCTCGt         >  1:241286/1‑101 (MQ=255)                                                                                            cGGAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATCCGCGGCAACCTCGTg        >  2:851923/1‑101 (MQ=255)                                                                                            cGGAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATCCGCGGCAACCTCGTg        >  1:8434188/1‑101 (MQ=255)                                                                                             ggAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGGGAAATCTCACCCCTCGAGCCAGACCAGAATCATCCCTGAAAGCCCATCCGCGGCaaaccctggg       >  1:2211246/1‑93 (MQ=38)                                                                                             ggAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCCGTCCAGAATCATCCCTGAAAGCCCATCCGCGGCAACCCCGTgg       >  1:2289792/1‑101 (MQ=255)                                                                                             ggAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATCCGCGGCAACCTCGTgg       >  1:1116284/1‑101 (MQ=255)                                                                                             ggAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATCCGCGGCAACCTCGTgg       >  1:2427221/1‑101 (MQ=255)                                                                                             ggAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATCCGCGGCAACCTCGTgg       >  2:6882453/1‑101 (MQ=255)                                                                                             ggAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATCCGCGGCAACCTCGTgg       >  1:287619/1‑101 (MQ=255)                                                                                             ggAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATCCGCGGCAACCTCGTgg       >  2:6353634/1‑101 (MQ=255)                                                                                             ggAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATCCGCGGCAACCTCGTgg       >  1:3299662/1‑101 (MQ=255)                                                                                              gAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATCCGCGGCAACCTCGTGGa      >  1:3461889/1‑101 (MQ=255)                                                                                              gAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATCCGCGGCAACCTCGTGGa      >  1:1781942/1‑101 (MQ=255)                                                                                              gAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATCCGCGGCAACCTCGTGGa      >  2:2367529/1‑101 (MQ=255)                                                                                                 ccccccGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATCCGCGGCAACCTCGTGGATCa   >  2:7897841/1‑101 (MQ=255)                                                                                                  cccccGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATCCGCGGCAACCTCGTGGATCAg  >  1:5076585/1‑101 (MQ=255)                                                                                                    |                                                                                                   CAAGATGAAATGAATGACTTTCCGTCTGCGGCGGTACAGCCTGTCCCACCCCTGCTTTTCCTTGGCAAAATACGCCGGGGGGTGCGGGGGGCGGAGCCCCCCGCGGGTCGCCTCGGGCCTGCCCGAGGCGAAATCTCACCCCTCGAGCCAGTCCAGAATCATCCCTGAAAGCCCATCCGCGGCAACCTCGTGGATCAG  >  NC_014034/744692‑744889 Base quality scores:  ATCG  < 3 ≤  ATCG  < 8 ≤  ATCG  < 18 ≤  ATCG  < 28 ≤  ATCG  < 38 ≤  ATCG