Organism : Rhodobacter sphaeroides 2.4.1 | Module List :
RSP_0591

Cold-shock protein (NCBI)

CircVis
Functional Annotations (3)
Function System
Cold shock proteins cog/ cog
DNA binding go/ molecular_function
regulation of transcription, DNA-dependent go/ biological_process
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for RSP_0591
(Mouseover regulator name to see its description)

RSP_0591 is regulated by 23 influences and regulates 16 modules.
Regulators for RSP_0591 (23)
Regulator Module Operator
RSP_0591 327 tf
RSP_1032 327 tf
RSP_1055 327 tf
RSP_1669 327 tf
RSP_1776 327 tf
RSP_1871 327 tf
RSP_1945 327 tf
RSP_2494 327 tf
RSP_3022 327 tf
RSP_3109 327 tf
RSP_3324 327 tf
RSP_3621 327 tf
RSP_3694 327 tf
RSP_0186 56 tf
RSP_0316 56 tf
RSP_0591 56 tf
RSP_1139 56 tf
RSP_1945 56 tf
RSP_1952 56 tf
RSP_2324 56 tf
RSP_2494 56 tf
RSP_3620 56 tf
RSP_3621 56 tf

Warning: RSP_0591 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
7832 1.80e-08 TTtccTTgCggcTtcaGTtGcgac
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7833 3.30e-05 tcctCtttcgAaCTT
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8362 9.20e-02 AAAAAC
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8363 4.40e-01 AttGacGaCaCGGtGaaAAcTCaA
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for RSP_0591

RSP_0591 is enriched for 3 functions in 3 categories.
Enrichment Table (3)
Function System
Cold shock proteins cog/ cog
DNA binding go/ molecular_function
regulation of transcription, DNA-dependent go/ biological_process
Module neighborhood information for RSP_0591

RSP_0591 has total of 47 gene neighbors in modules 56, 327
Gene neighbors (47)
Gene Common Name Description Module membership
RSP_0091 smoE ABC sorbitol/mannitol transporter, periplasmic binding protein (NCBI) 56, 64
RSP_0092 smoF ABC sorbitol/mannitol transporter, inner membrane subunit (NCBI) 56, 64
RSP_0093 smoG ABC sorbitol/mannitol transporter, inner membrane subunit (NCBI) 56, 64
RSP_0094 smoK ABC sorbitol/mannitol transporter, ATPase subunit (NCBI) 56, 64
RSP_0095 smoS Sorbitol dehydrogenase (NCBI) 56, 64
RSP_0096 mtlK Mannitol dehydrogenase (NCBI) 56, 64
RSP_0205 RSP_0205 hypothetical protein (NCBI) 213, 327
RSP_0220 RSP_0220 hypothetical protein (NCBI) 68, 327
RSP_0370 RSP_0370 hypothetical protein (NCBI) 210, 327
RSP_0591 RSP_0591 Cold-shock protein (NCBI) 56, 327
RSP_0626 infA Probable translation initiation factor 1 (IF-1) (NCBI) 75, 327
RSP_0718 rpsU 30S ribosomal protein S21 (RpsU) (NCBI) 48, 56
RSP_0766 RSP_0766 hypothetical protein (NCBI) 10, 56
RSP_0867 rnhA ribonuclease H (NCBI) 213, 327
RSP_1059 rpmH 50S ribosomal protein L34 (NCBI) 75, 327
RSP_1104 RSP_1104 hypothetical protein (NCBI) 35, 327
RSP_1133 RSP_1133 hypothetical protein (NCBI) 56, 341
RSP_1201 RSP_1201 hypothetical protein (NCBI) 43, 327
RSP_1341 rpsT Ribosomal protein S20 (NCBI) 75, 327
RSP_1388 RSP_1388 DNA-binding protein HU, form N (NCBI) 327, 345
RSP_1398 rpmJ ribosomal protein L36 (NCBI) 75, 327
RSP_1496 RSP_1496 hypothetical protein (NCBI) 181, 327
RSP_1498 RSP_1498 hypothetical protein (NCBI) 181, 327
RSP_1517 spbA Histone-like protein of HNS family (NCBI) 48, 327
RSP_1529 trxA Thioredoxin (NCBI) 327, 345
RSP_1536 RSP_1536 conserved hypothetical protein that may be involved in lipid metabolism (NCBI) 56, 101
RSP_1669 rpoZ RNA polymerase omega subunit (NCBI) 75, 327
RSP_1887 RSP_1887 Ribosomal protein L33 (NCBI) 75, 327
RSP_1951 RSP_1951 hypothetical protein (NCBI) 56, 229
RSP_1952 RSP_1952 Cold-shock DNA-binding domain protein (NCBI) 56, 229
RSP_2016 rpmB 50S ribosomal protein L28 (NCBI) 248, 327
RSP_2024 cspA Cold shock protein cspA (NCBI) 56, 229
RSP_2305 RSP_2305 MaoC family protein (NCBI) 56, 140
RSP_2424 RSP_2424 ferredoxin II (NCBI) 75, 327
RSP_2463 acpP Acyl carrier protein (ACP) (NCBI) 327, 345
RSP_2843 hfq Host factor I protein (NCBI) 56, 256
RSP_3599 RSP_3599 hypothetical protein (NCBI) 56, 200
RSP_3620 RSP_3620 Cold-shock DNA-binding protein (NCBI) 56, 140
RSP_3621 RSP_3621 Cold-shock DNA-binding protein (NCBI) 56, 229
RSP_3622 RSP_3622 hypothetical protein (NCBI) 56, 229
RSP_4300 RSP_4300 tRNA-Ser (NCBI) 327, 336
RSP_4301 RSP_4301 tRNA-Leu (NCBI) 48, 327
RSP_4309 RSP_4309 tRNA-Val (NCBI) 75, 327
RSP_4311 RSP_4311 tRNA-Pro (NCBI) 75, 327
RSP_4323 RSP_4323 tRNA-OTHER (NCBI) 75, 327
RSP_4332 RSP_4332 tRNA-Leu (NCBI) 75, 327
RSP_6201 RSP_6201 hypothetical protein (NCBI) 56, 276
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for RSP_0591
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend