Organism : Rhodobacter sphaeroides 2.4.1 | Module List :
RSP_2232

putative uracil-DNA glycosylase (NCBI)

CircVis
Functional Annotations (3)
Function System
Uracil-DNA glycosylase cog/ cog
DNA-directed DNA polymerase activity go/ molecular_function
UDG_fam_dom tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for RSP_2232
(Mouseover regulator name to see its description)

RSP_2232 is regulated by 23 influences and regulates 0 modules.
Regulators for RSP_2232 (23)
Regulator Module Operator
RSP_1518 193 tf
RSP_1590 193 tf
RSP_1790 193 tf
RSP_1867 193 tf
RSP_2610 193 tf
RSP_2867 193 tf
RSP_3238 193 tf
RSP_3322 193 tf
RSP_3400 193 tf
RSP_3464 193 tf
RSP_3684 193 tf
RSP_1014 38 tf
RSP_1055 38 tf
RSP_1231 38 tf
RSP_1607 38 tf
RSP_1776 38 tf
RSP_2780 38 tf
RSP_2867 38 tf
RSP_2882 38 tf
RSP_3322 38 tf
RSP_3339 38 tf
RSP_3528 38 tf
RSP_3684 38 tf

Warning: RSP_2232 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
7796 1.70e-02 aCctGAacgtC
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7797 5.20e+03 aagaAACAG.t.cGaAaaTaTT
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8106 3.70e-02 AacaaAtTGAGaA
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8107 3.50e+02 TTaAccTT
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for RSP_2232

RSP_2232 is enriched for 3 functions in 3 categories.
Enrichment Table (3)
Function System
Uracil-DNA glycosylase cog/ cog
DNA-directed DNA polymerase activity go/ molecular_function
UDG_fam_dom tigr/ tigrfam
Module neighborhood information for RSP_2232

RSP_2232 has total of 57 gene neighbors in modules 38, 193
Gene neighbors (57)
Gene Common Name Description Module membership
RSP_0117 RSP_0117 Cytochrome c oxidase, subunit I (NCBI) 38, 238
RSP_0151 RSP_0151 hypothetical protein (NCBI) 38, 74
RSP_0172 RSP_0172 Predicted transcriptional regulators (NCBI) 38, 62
RSP_0181 RSP_0181 ABC nitrate/sulfonate/bicarbonate transporter, inner membrane subunit (NCBI) 193, 342
RSP_0212 RSP_0212 HNH nuclease (NCBI) 74, 193
RSP_0213 RSP_0213 Phage terminase-like protein large subunit (NCBI) 193, 325
RSP_0362 RSP_0362 hypothetical protein (NCBI) 38, 320
RSP_0364 RSP_0364 possible transporter, RarD family, DMT superfamily (NCBI) 38, 234
RSP_0519 RSP_0519 NAD-dependent epimerase/dehydratase (NCBI) 38, 217
RSP_0520 RSP_0520 NAD-dependent dehydratase/epimerase (NCBI) 38, 217
RSP_0615 RSP_0615 Putative oxidoreductase (NCBI) 138, 193
RSP_1647 RSP_1647 probable peptidase (NCBI) 191, 193
RSP_1648 RSP_1648 hypothetical protein (NCBI) 193, 355
RSP_1695 RSP_1695 hypothetical protein (NCBI) 38, 124
RSP_1821 RSP_1821 Molybdopterin-containing oxidoreductase, probable formate dehydrogenase (NCBI) 38, 137
RSP_1888 RSP_1888 possible acetyltransferase (GNAT) family (NCBI) 38, 62
RSP_2074 RSP_2074 hypothetical protein (NCBI) 38, 379
RSP_2140 RSP_2140 hypothetical protein (NCBI) 19, 38
RSP_2166 putA Bifunctional putA protein; Proline dehydrogenase / Delta-1-pyrroline-5-carboxylate dehydrogenase (NCBI) 38, 251
RSP_2232 RSP_2232 putative uracil-DNA glycosylase (NCBI) 38, 193
RSP_2466 RSP_2466 hypothetical protein (NCBI) 193, 235
RSP_2467 RSP_2467 Putative large terminase (NCBI) 191, 193
RSP_2468 RSP_2468 putative portal protein (NCBI) 193, 236
RSP_2469 RSP_2469 hypothetical protein (NCBI) 193, 236
RSP_2470 RSP_2470 putative prohead protease (NCBI) 193, 236
RSP_2471 RSP_2471 Predicted phage phi-C31 gp36 major capsid-like protein (NCBI) 193, 235
RSP_2472 RSP_2472 hypothetical protein (NCBI) 193, 235
RSP_2473 RSP_2473 hypothetical protein (NCBI) 191, 193
RSP_2475 RSP_2475 putative phage tail minor protein (NCBI) 191, 193
RSP_2477 RSP_2477 hypothetical protein (NCBI) 189, 193
RSP_2478 RSP_2478 hypothetical protein (NCBI) 193, 235
RSP_2480 RSP_2480 hypothetical protein (NCBI) 193, 235
RSP_2575 RSP_2575 hypothetical protein (NCBI) 38, 124
RSP_2672 RSP_2672 hypothetical protein (NCBI) 38, 282
RSP_2720 RSP_2720 Putative LysE/RhtB family amino acid efflux pump (NCBI) 38, 325
RSP_2721 RSP_2721 hypothetical protein (NCBI) 38, 370
RSP_2794 RSP_2794 Putative monooxygenase beta subunit (NCBI) 103, 193
RSP_2795 RSP_2795 Putative regulatory protein of multicomponent monooxygenase (NCBI) 103, 193
RSP_3017 RSP_3017 nitrilotriacetate monooxygenase (NCBI) 38, 83
RSP_3020 RSP_3020 hypothetical protein (NCBI) 38, 62
RSP_3030 RSP_3030 ABC peptide/opine transporter, inner membrane subunit (NCBI) 193, 282
RSP_3032 RSP_3032 ABC peptide/opine transporter, ATPase subunit (NCBI) 193, 282
RSP_3033 RSP_3033 ABC peptide/opine transporter, ATPase subunit (NCBI) 193, 282
RSP_3034 RSP_3034 ABC peptide/opine transporter, periplasmic substrate-binding protein (NCBI) 193, 282
RSP_3036 RSP_3036 hypothetical protein (NCBI) 38, 141
RSP_3061 RSP_3061 hypothetical protein (NCBI) 38, 141
RSP_3068 RSP_3068 hypothetical protein (NCBI) 38, 307
RSP_3118 RSP_3118 hypothetical protein (NCBI) 38, 313
RSP_3119 RSP_3119 conserved hypothetical protein / putative esterase (NCBI) 14, 38
RSP_3180 RSP_3180 Transglutaminase-like enzyme (NCBI) 38, 97
RSP_3339 RSP_3339 transcriptional regulator, GntR family (NCBI) 38, 253
RSP_3355 RSP_3355 putative tape measure protein (NCBI) 38, 300
RSP_3424 RSP_3424 hypothetical protein (NCBI) 38, 285
RSP_3499 RSP_3499 H+-transporting two-sector ATPase, alpha/beta subunit (NCBI) 38, 62
RSP_3617 RSP_3617 hypothetical protein (NCBI) 38, 62
RSP_3654 RSP_3654 None 193, 292
RSP_3837 RSP_3837 hypothetical protein (NCBI) 38, 196
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for RSP_2232
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend