Rv0157 NAD(P) transhydrogenase subunit beta (EC 1.6.1.2)

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0157 pntB NAD(P) transhydrogenase subunit beta (EC 1.6.1.2) CDS 185052 186479 + 1 428 475 FALSE

Rv0157 (NAD(P) transhydrogenase subunit beta (EC 1.6.1.2)) is predicted to be co-regulated in modules bicluster_0100 with residual 0.55 and bicluster_0381 with residual 0.54.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 130.00 and 310.00 for bicluster_0100 and 0.18 and 6,300.00 for bicluster_0381 respectively.

These modules are enriched for following go terms: oxidoreductase activity, oxidoreductase activity, acting on NAD(P... inorganic anion transport, secondary active transmembrane transport....

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 17:54
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-15669 MT0165 2610
Product (LegacyBRC) Product (RefSeq)
PROBABLE NAD[P] TRANSHYDROGENASE [SUBUNIT BETA] PNTB [INTEGRAL MEMBRANE PROTEIN] [PYRIDINE NUCLEOTIDE TRANSHYDROGENASE SUBUNIT BETA] [NICOTINAMIDE NUCLEOTIDE TRANSHYDROGENASE SUBUNIT BETA] NAD(P) transhydrogenase subunit beta
Operon # Operon
111 - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Nicotinate and nicotinamide metabolism

16
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607299 NP_214671.1 Run
GO:0008750

NAD(P)+ transhydrogenase (AB-specific) activity

NAD(P)+ transhydrogenase (AB-specific) activity

Details: 
Catalysis of the reaction: NADPH + H+ + NAD+ = NADP+ + NADH + H+. The reaction is A-specific (i.e. the pro-R hydrogen is transferred from the 4-position of reduced nicotinamide cofactor) with respect to NAD+ and B-specific (i.e. the pro-S hydrogen is transferred) with respect to NADP+.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0005887

integral to plasma membrane

integral to plasma membrane

Details: 
Penetrating at least one phospholipid bilayer of a plasma membrane. May also refer to the state of being buried in the bilayer with no exposure outside the bilayer.
GO Category: 
cellular_component
101
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.080000 0.36

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: