Rv0208c tRNA (guanine46-N7-)-methyltransferase (EC


Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0208c yggH tRNA (guanine46-N7-)-methyltransferase (EC CDS 248115 248906 - 792 263 FALSE

Rv0208c (tRNA (guanine46-N7-)-methyltransferase (EC is predicted to be co-regulated in modules bicluster_0231 with residual 0.47 and bicluster_0408 with residual 0.55.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 3.10 and 6,500.00 for bicluster_0231 and 0.01 and 0.62 for bicluster_0408 respectively.

These modules are enriched for following go terms: fatty acid synthase activity.

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Distance to Tuberculist Start Codon Internal TSS New Internal UTR Re-Annotated Start Tuberculist Annotated Start
-0.058 18 248888 180 248708 248906
Product (LegacyBRC) Product (RefSeq)
tRNA [guanine-N[7]-]-methyltransferase tRNA (guanine-N(7)-)-methyltransferase
Operon # Operon
144 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics


Not assigned Not assigned
Locus Tuberculist Genome View


Locus Tag KEGG Pathways


not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network


GI Number Protein ID Blast Conserved Domains
15607349 NP_214722.1 Run

tRNA (guanine-N7-)-methyltransferase activity

tRNA (guanine-N7-)-methyltransferase activity

Catalysis of the reaction: S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N7-methylguanine.
GO Category: 
Total items in this category:  



The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.240000 0.14

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: