Rv0245 Nitrilotriacetate monooxygenase component B (EC 1.14.13.-)


Product Feature Type Start End Strand Length AA Length is TF
Rv0245 Nitrilotriacetate monooxygenase component B (EC 1.14.13.-) CDS 296005 296493 + 489 162 FALSE

Rv0245 (Nitrilotriacetate monooxygenase component B (EC 1.14.13.-)) is predicted to be co-regulated in modules bicluster_0350 with residual 0.37 and bicluster_0593 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 1,000.00 and 4,000.00 for bicluster_0350 and 4,900.00 and 7,400.00 for bicluster_0593 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Operon # Operon
Locus Tuberculist Genome View


Locus Tag KEGG Pathways


not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network


GI Number Protein ID Blast Conserved Domains
15607386 NP_214759.1 Run
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.170000 0.07

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: