Rv0351 Heat shock protein GrpE

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0351 grpE Heat shock protein GrpE CDS 421709 422416 + 708 235 FALSE

Rv0351 (Heat shock protein GrpE) is predicted to be co-regulated in modules bicluster_0402 with residual 0.39 and bicluster_0475 with residual 0.40.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 650.00 for bicluster_0402 and 0.00 and 9.10 for bicluster_0475 respectively.

These modules are enriched for following go terms: fatty acid synthase activity.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Protein grpE GRPE protein (HSP-70 cofactor)
Operon # Operon
241 - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607492 NP_214865.1 Run
GO:0051087

chaperone binding

chaperone binding

Details: 
Interacting selectively and non-covalently with a chaperone protein, a class of proteins that bind to nascent or unfolded polypeptides and ensure correct folding or transport.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0000774

adenyl-nucleotide exchange factor activity

adenyl-nucleotide exchange factor activity

Details: 
Stimulates the hydrolysis and exchange of adenyl nucleotides by other proteins.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0006457

protein folding

protein folding

Details: 
The process of assisting in the covalent and noncovalent assembly of single chain polypeptides or multisubunit complexes into the correct tertiary structure.
GO Category: 
biological_process
7
Total items in this category:  
GO:0042803

protein homodimerization activity

protein homodimerization activity

Details: 
Interacting selectively and non-covalently with an identical protein to form a homodimer.
GO Category: 
molecular_function
83
Total items in this category:  
GO:0009267

cellular response to starvation

cellular response to starvation

Details: 
Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of deprivation of nourishment.
GO Category: 
biological_process
9
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: