Rv0353 HspR, transcriptional repressor of DnaK operon


Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0353 hspR HspR, transcriptional repressor of DnaK operon CDS 423639 424019 + 381 126 TRUE

Rv0353 (HspR, transcriptional repressor of DnaK operon) is predicted to be co-regulated in modules bicluster_0402 with residual 0.39 and bicluster_0475 with residual 0.40.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 650.00 for bicluster_0402 and 0.00 and 9.10 for bicluster_0475 respectively.

These modules are enriched for following go terms: fatty acid synthase activity.

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 4 of 4
Gene Target Differential Expression Distance Expression pvalue Type
Nitrite reductase [NAD(P)H] large subunit (EC
No -130 -0.14 0.771506 CDS
Chaperone protein DnaK
No -31 -0.72 0.0717048 Primary.TSS
ClpB protein
No 47 0.31 0.376743 Primary.TSS
Flavohemoprotein (Hemoglobin-like protein) (Flavohemoglobin) (Nitric oxide dioxygenase) (EC
No -64 -0.29 0.360026 Primary.TSS
Displaying 1 - 2 of 2
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator, TetR family
No -17 -0.42 0.256886 Primary.TSS
Mycofactocin system transcriptional regulator
No 52 0.31 0.999588 Primary.TSS
Product (LegacyBRC) Product (RefSeq)
Operon # Operon
241 - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics


Not assigned Not assigned
Locus Tuberculist Genome View


Locus Tag KEGG Pathways


not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network


GI Number Protein ID Blast Conserved Domains
15607494 NP_214867.1 Run

plasma membrane

plasma membrane

The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
Total items in this category:  

negative regulation of transcription, DNA-dependent

negative regulation of transcription, DNA-dependent

Any process that stops, prevents, or reduces the frequency, rate or extent of cellular DNA-dependent transcription.
GO Category: 
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
BioProject Accession GEO Series References Repository Sample Method Sample Type
PRJNA254351 GSM1426471 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426472 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426473 GSE59086 25232098 GEO Tiling Array RNA
Experiment UCSC Genome Browser
Rv0353_B608 UCSC Browser Tracks

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.010000 0.37

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated: 0.230658
p-value INH: 0.259123
Displaying 1 - 17 of 17
Condition Count Day Doublings Fitness U.I Plots
D0U 27 0 0.00 10.41 U
D3I 3 3 3.83 9.76 I
D3U 3 3 3.83 9.46 U
D5I 9 5 6.00 10.90 I
D5U 17 5 6.00 11.27 U
D7I 18 7 8.14 10.13 I
D7U 19 7 8.14 10.07 U
D14I 4 14 15.63 4.85 I
D14U 4 14 15.63 7.79 U
D17I 3 17 19.15 4.19 I
D17U 3 17 19.15 7.55 U
D21I 4 21 23.23 3.12 I
D21U 4 21 23.23 7.64 U
D24I 3 24 26.60 2.10 I
D24U 3 24 26.60 7.47 U
D28I 4 28 30.61 2.49 I
D28U 4 28 30.61 7.25 U