Rv0512 Porphobilinogen synthase (EC


Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0512 hemB Porphobilinogen synthase (EC CDS 604602 605591 + 990 329 FALSE

Rv0512 (Porphobilinogen synthase (EC is predicted to be co-regulated in modules bicluster_0135 with residual 0.54 and bicluster_0581 with residual 0.61.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 3.10 and 2,200.00 for bicluster_0135 and 0.01 and 120.00 for bicluster_0581 respectively.

These modules are enriched for following go terms: small molecule biosynthetic process, single-organism biosynthetic process, aromatic compound biosynthetic process, heterocycle biosynthetic process, organic cyclic compound biosynthetic pro... .

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Re-Annotated Start Tuberculist Annotated Start
-0.379 604608 604602
Product (LegacyBRC) Product (RefSeq)
Delta-aminolevulinic acid dehydratase delta-aminolevulinic acid dehydratase
Operon # Operon
346 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics


Porphobilinogen synthase Porphyrin and chlorophyll metabolism
Locus Tuberculist Genome View


Locus Tag KEGG Pathways


Porphyrin and chlorophyll metabolism

Total items in this category:  


Metabolic pathways

Total items in this category:  


Biosynthesis of secondary metabolites

Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network


GI Number Protein ID Blast Conserved Domains
15607653 NP_215026.1 Run

porphobilinogen synthase activity

porphobilinogen synthase activity

Catalysis of the reaction: 2 5-aminolevulinate = 2 H(2)O + H(+) + porphobilinogen.
GO Category: 
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: