Rv0751c 3-hydroxyisobutyrate dehydrogenase (EC


Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0751c mmsB 3-hydroxyisobutyrate dehydrogenase (EC CDS 842347 843231 - 885 294 FALSE

Rv0751c (3-hydroxyisobutyrate dehydrogenase (EC is predicted to be co-regulated in modules bicluster_0035 with residual 0.52 and bicluster_0401 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.01 and 130.00 for bicluster_0035 and 100.00 and 180.00 for bicluster_0401 respectively.

These modules are enriched for following go terms: hydroxymethyl-, formyl- and related tran... .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 05:58
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18373 MT0775 2123
Product (LegacyBRC) Product (RefSeq)
Probable 3-hydroxyisobutyrate dehydrogenase 3-hydroxyisobutyrate dehydrogenase MmsB
Operon # Operon
498 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics


3-hydroxyisobutyrate dehydrogenase Valine, leucine and isoleucine degradation
Locus Tuberculist Genome View


Locus Tag KEGG Pathways


Valine, leucine and isoleucine degradation

Total items in this category:  


Metabolic pathways

Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network


GI Number Protein ID Blast Conserved Domains
15607891 NP_215265.1 Run

3-hydroxyisobutyrate dehydrogenase activity

3-hydroxyisobutyrate dehydrogenase activity

Catalysis of the reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+.
GO Category: 
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.140000 0.29

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: