Rv0812 Aminodeoxychorismate lyase (EC 4.1.3.38)

Summary

Product Feature Type Start End Strand Length AA Length is TF
Rv0812 Aminodeoxychorismate lyase (EC 4.1.3.38) CDS 906423 907292 + 870 289 FALSE

Rv0812 (Aminodeoxychorismate lyase (EC 4.1.3.38)) is predicted to be co-regulated in modules bicluster_0390 with residual 0.52 and bicluster_0493 with residual 0.66.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.64 and 23.00 for bicluster_0390 and 0.02 and 25.00 for bicluster_0493 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score New Primary UTR Primary TSS Re-Annotated Start Tuberculist Annotated Start
-0.855 2 906403 906405 906423
Product (LegacyBRC) Product (RefSeq)
PROBABLE AMINO ACID AMINOTRANSFERASE 4-amino-4-deoxychorismate lyase
Operon # Operon
537
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Aminodeoxychorismate lyase Folate biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Folate biosynthesis

10
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57116785 YP_177757.1 Run
GO:0008696

4-amino-4-deoxychorismate lyase activity

4-amino-4-deoxychorismate lyase activity

Details: 
Catalysis of the reaction: 4-amino-4-deoxychorismate = 4-aminobenzoate + H(+) + pyruvate.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: