Rv0846c Multicopper oxidase

Summary

Product Feature Type Start End Strand Length AA Length is TF
Rv0846c Multicopper oxidase CDS 942680 944194 - 1 515 504 FALSE

Rv0846c (Multicopper oxidase) is predicted to be co-regulated in modules bicluster_0267 with residual 0.52 and bicluster_0337 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.05 for bicluster_0267 and 0.00 and 0.00 for bicluster_0337 respectively.

These modules are enriched for following go terms: carboxylic acid biosynthetic process, organic acid biosynthetic process, histidine biosynthetic process, histidine metabolic process, imidazole-containing compound metabolic ..., cellular amino acid metabolic process, small molecule biosynthetic process, single-organism biosynthetic process, aromatic amino acid family biosynthetic ... aspartate carbamoyltransferase activity.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
PROBABLE OXIDASE oxidase
Operon # Operon
566
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607986 NP_215361.1 Run
GO:0016491

oxidoreductase activity

oxidoreductase activity

Details: 
Catalysis of an oxidation-reduction (redox) reaction, a reversible chemical reaction in which the oxidation state of an atom or atoms within a molecule is altered. One substrate acts as a hydrogen or electron donor and becomes oxidized, while the other acts as hydrogen or electron acceptor and becomes reduced.
GO Category: 
molecular_function
12
Total items in this category:  
GO:0005507

copper ion binding

copper ion binding

Details: 
Interacting selectively and non-covalently with copper (Cu) ions.
GO Category: 
molecular_function
2
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.550000 1.52

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: