Rv0971c Methylglutaconyl-CoA hydratase (EC 4.2.1.18)

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0971c echA7 Methylglutaconyl-CoA hydratase (EC 4.2.1.18) CDS 1081775 1082584 - 810 269 FALSE

Rv0971c (Methylglutaconyl-CoA hydratase (EC 4.2.1.18)) is predicted to be co-regulated in modules bicluster_0134 with residual 0.52 and bicluster_0326 with residual 0.49.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 9.50 for bicluster_0134 and 0.00 and 0.40 for bicluster_0326 respectively.

These modules are enriched for following go terms: oxidoreductase activity, acting on other... .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
PROBABLE ENOYL-CoA HYDRATASE ECHA7 [ENOYL HYDRASE] [UNSATURATED ACYL-CoA HYDRATASE] [CROTONASE] enoyl-CoA hydratase
Operon # Operon
649 - - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Methylglutaconyl-CoA hydratase Valine, leucine and isoleucine degradation
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Fatty acid metabolism

51
Total items in this category:  

KEGG

Valine, leucine and isoleucine degradation

60
Total items in this category:  

KEGG

Geraniol degradation

52
Total items in this category:  

KEGG

Lysine degradation

46
Total items in this category:  

KEGG

Benzoate degradation

48
Total items in this category:  

KEGG

Tryptophan metabolism

47
Total items in this category:  

KEGG

beta-Alanine metabolism

37
Total items in this category:  

KEGG

Aminobenzoate degradation

73
Total items in this category:  

KEGG

Propanoate metabolism

62
Total items in this category:  

KEGG

Butanoate metabolism

63
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608111 NP_215486.1 Run
GO:0004490

methylglutaconyl-CoA hydratase activity

methylglutaconyl-CoA hydratase activity

Details: 
Catalysis of the reaction: (S)-3-hydroxy-3-methylglutaryl-CoA = trans-3-methylglutaconyl-CoA + H(2)O.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.310000 0.81

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: