Rv1077 Cystathionine beta-synthase (EC 4.2.1.22)

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1077 cbs Cystathionine beta-synthase (EC 4.2.1.22) CDS 1201717 1203111 + 1 395 464 FALSE

Rv1077 (Cystathionine beta-synthase (EC 4.2.1.22)) is predicted to be co-regulated in modules bicluster_0062 with residual 0.51 and bicluster_0440 with residual 0.53.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 18.00 and 490.00 for bicluster_0062 and 0.01 and 67.00 for bicluster_0440 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 11:08
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18697 MT1108 1674
Product (LegacyBRC) Product (RefSeq)
Probable cystathionine beta-synthase CBS [Serine sulfhydrase] [Beta-thionase] [Hemoprotein H-450] cystathionine beta-synthase CBS (Serine sulfhydrase) (Beta-thionase) (hemoprotein H-450)
Operon # Operon
725
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Glycine, serine and threonine metabolism

27
Total items in this category:  

KEGG

Cysteine and methionine metabolism

28
Total items in this category:  

KEGG

Selenocompound metabolism

40
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57116820 YP_177782.1 Run
GO:0004124

cysteine synthase activity

cysteine synthase activity

Details: 
Catalysis of the reaction: O3-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate.
GO Category: 
molecular_function
4
Total items in this category:  
GO:0005576

extracellular region

extracellular region

Details: 
The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
GO Category: 
cellular_component
272
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.610000 1.47

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: