Rv1145

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1145 mmpL13a CDS 1272423 1273334 + 912 303 FALSE

Rv1145 () is predicted to be co-regulated in modules bicluster_0362 with residual 0.57 and bicluster_0442 with residual 0.47.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 32.00 and 150.00 for bicluster_0362 and 0.00 and 0.00 for bicluster_0442 respectively.

These modules are enriched for following go terms: nicotinamide nucleotide metabolic proces..., oxidoreduction coenzyme metabolic proces..., pyridine nucleotide metabolic process, glucose catabolic process, hexose catabolic process, monosaccharide catabolic process, pyridine-containing compound metabolic p..., organic substance catabolic process, catabolic process, oxidoreductase activity, acting on the C..., oxidoreductase activity, acting on CH-OH..., transferase activity, transferring aldeh....

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
PROBABLE CONSERVED TRANSMEMBRANE TRANSPORT PROTEIN MMPL13A transmembrane transport protein MmpL13A
Operon # Operon
775 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics
Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608285 NP_215661.1 Run
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.770000 1.10

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: