Rv1263 Amidase (EC


Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1263 amiB2 Amidase (EC CDS 1410431 1411819 + 1 389 462 FALSE

Rv1263 (Amidase (EC is predicted to be co-regulated in modules bicluster_0303 with residual 0.47 and bicluster_0535 with residual 0.57.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 18,000.00 and 1,800.00 for bicluster_0303 and 11.00 and 3,000.00 for bicluster_0535 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 11:16
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18084 MT1301 1364
Product (LegacyBRC) Product (RefSeq)
Putative amidase amiB2 amidase
Operon # Operon
Locus Tuberculist Genome View


Locus Tag KEGG Pathways


Arginine and proline metabolism

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Phenylalanine metabolism

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Tryptophan metabolism

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Cyanoamino acid metabolism

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Aminobenzoate degradation

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Styrene degradation

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Microbial metabolism in diverse environments

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BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network


GI Number Protein ID Blast Conserved Domains
15608403 NP_215779.1 Run
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.940000 1.58

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: