Rv1296 Homoserine kinase (EC 18.104.22.168)
These modules are enriched for following go terms: damaged DNA binding, cysteine-type endopeptidase activity, cysteine-type peptidase activity cellular macromolecule catabolic process, nucleobase-containing compound catabolic..., aromatic compound catabolic process, macromolecule catabolic process, exonuclease activity, active with either..., deoxyribonuclease activity, oxidoreductase activity, acting on paire..., exonuclease activity.
This gene is found to be for growth on cholesterol.
|Product (LegacyBRC)||Product (RefSeq)|
|Homoserine kinase||homoserine kinase|
|BioCyc Gene Page||Cellular Overview Map|
homoserine kinase activity
Quantitative Proteomics Data
|t-test p-value||Cholesterol/Glycerol Ratio|
How essentiality calculations were done?
The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.
TRIP log2 fold abundance change
reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.