Rv1316c Methylated-DNA--protein-cysteine methyltransferase (EC

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1316c ogt Methylated-DNA--protein-cysteine methyltransferase (EC CDS 1477134 1477631 - 498 165 FALSE

Rv1316c (Methylated-DNA--protein-cysteine methyltransferase (EC is predicted to be co-regulated in modules bicluster_0261 with residual 0.52 and bicluster_0560 with residual 0.59.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.03 and 260.00 for bicluster_0261 and 0.01 and 21.00 for bicluster_0560 respectively.

These modules are enriched for following go terms: S-methyltransferase activity, signal transducer activity, molecular transducer activity folic acid-containing compound biosynthe..., pteridine-containing compound biosynthet..., coenzyme metabolic process, cellular modified amino acid biosyntheti....

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 11:16
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18839 MT1357 3139
Product (LegacyBRC) Product (RefSeq)
Methylated-DNA--protein-cysteine methyltransferase methylated-DNA--protein-cysteine methyltransferase
Operon # Operon
892 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics


Not assigned Not assigned
Locus Tuberculist Genome View


Locus Tag KEGG Pathways


not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network


GI Number Protein ID Blast Conserved Domains
15608456 NP_215832.1 Run

methylated-DNA-[protein]-cysteine S-methyltransferase activity

methylated-DNA-[protein]-cysteine S-methyltransferase activity

Catalysis of the reaction: DNA (containing 6-O-methylguanine) + (protein)-L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.
GO Category: 
Total items in this category:  

plasma membrane

plasma membrane

The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.820000 1.46

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: