Rv1389 Guanylate kinase (EC 2.7.4.8)

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1389 gmk Guanylate kinase (EC 2.7.4.8) CDS 1564401 1565027 + 627 208 FALSE

Rv1389 (Guanylate kinase (EC 2.7.4.8)) is predicted to be co-regulated in modules bicluster_0195 with residual 0.47 and bicluster_0400 with residual 0.51.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0195 and 0.01 and 0.12 for bicluster_0400 respectively.

These modules are enriched for following go terms: macromolecule metabolic process, nucleic acid metabolic process carboxypeptidase activity, thiosulfate sulfurtransferase activity, unfolded protein binding, exopeptidase activity.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Guanylate kinase guanylate kinase
Operon # Operon
931
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Guanylate kinase Purine metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Purine metabolism

65
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608528 NP_215905.1 Run
GO:0004385

guanylate kinase activity

guanylate kinase activity

Details: 
Catalysis of the reaction: ATP + GMP = ADP + GDP.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0000287

magnesium ion binding

magnesium ion binding

Details: 
Interacting selectively and non-covalently with magnesium (Mg) ions.
GO Category: 
molecular_function
52
Total items in this category:  
GO:0004017

adenylate kinase activity

adenylate kinase activity

Details: 
Catalysis of the reaction: ATP + AMP = 2 ADP.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0004385

guanylate kinase activity

guanylate kinase activity

Details: 
Catalysis of the reaction: ATP + GMP = ADP + GDP.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005524

ATP binding

ATP binding

Details: 
Interacting selectively and non-covalently with ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
GO Category: 
molecular_function
58
Total items in this category:  
GO:0019002

GMP binding

GMP binding

Details: 
Interacting selectively and non-covalently with GMP, guanosine monophosphate.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0019003

GDP binding

GDP binding

Details: 
Interacting selectively and non-covalently with GDP, guanosine 5'-diphosphate.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: