Rv1442 Biotin sulfoxide reductase (EC 1.-.-.-)

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1442 bisC Biotin sulfoxide reductase (EC 1.-.-.-) CDS 1619791 1622091 + 2 301 766 FALSE

Rv1442 (Biotin sulfoxide reductase (EC 1.-.-.-)) is predicted to be co-regulated in modules bicluster_0245 with residual 0.51 and bicluster_0395 with residual 0.54.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.15 and 240.00 for bicluster_0245 and 0.00 and 760.00 for bicluster_0395 respectively.

These modules are enriched for following go terms: oxidoreductase activity .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 11:16
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14765 MT1487 91
Product (LegacyBRC) Product (RefSeq)
PROBABLE BIOTIN SULFOXIDE REDUCTASE BISC [BDS reductase] [BSO reductase] biotin sulfoxide reductase
Operon # Operon
963
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608580 NP_215958.1 Run
GO:0016491

oxidoreductase activity

oxidoreductase activity

Details: 
Catalysis of an oxidation-reduction (redox) reaction, a reversible chemical reaction in which the oxidation state of an atom or atoms within a molecule is altered. One substrate acts as a hydrogen or electron donor and becomes oxidized, while the other acts as hydrogen or electron acceptor and becomes reduced.
GO Category: 
molecular_function
12
Total items in this category:  
GO:0030151

molybdenum ion binding

molybdenum ion binding

Details: 
Interacting selectively and non-covalently with molybdenum (Mo) ions.
GO Category: 
molecular_function
1
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.120000 1.35

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: