Rv1493 Methylmalonyl-CoA mutase (EC 5.4.99.2)

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1493 mutB Methylmalonyl-CoA mutase (EC 5.4.99.2) CDS 1684005 1686257 + 2 253 750 FALSE

Rv1493 (Methylmalonyl-CoA mutase (EC 5.4.99.2)) is predicted to be co-regulated in modules bicluster_0026 with residual 0.58 and bicluster_0558 with residual 0.61.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.18 and 1.60 for bicluster_0026 and 0.00 and 0.69 for bicluster_0558 respectively.

These modules are enriched for following go terms: response to stress, S-methyltransferase activity, O-acyltransferase activity.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 11:39
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14972 MT1540 384
Product (LegacyBRC) Product (RefSeq)
Probable methylmalonyl-CoA mutase large subunit methylmalonyl-CoA mutase
Operon # Operon
992 - - - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Valine, leucine and isoleucine degradation

60
Total items in this category:  

KEGG

Glyoxylate and dicarboxylate metabolism

31
Total items in this category:  

KEGG

Propanoate metabolism

62
Total items in this category:  

KEGG

Carbon fixation pathways in prokaryotes

42
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608631 NP_216009.1 Run
GO:0004494

methylmalonyl-CoA mutase activity

methylmalonyl-CoA mutase activity

Details: 
Catalysis of the reaction: (R)-methylmalonyl-CoA = succinyl-CoA.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0019678

propionate metabolic process, methylmalonyl pathway

propionate metabolic process, methylmalonyl pathway

Details: 
The chemical reactions and pathways involving propionate that occur in the methylmalonyl pathway.
GO Category: 
biological_process
2
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.150000 1.01

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: