Rv1777 Cytochrome P450 144

Summary

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv1777 cyp144 Cytochrome P450 144 CDS 2010656 2011960 + 1 305 434 FALSE

Rv1777 (Cytochrome P450 144) is predicted to be co-regulated in modules bicluster_0527 with residual 0.54 and bicluster_0555 with residual 0.43.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.47 and 120.00 for bicluster_0527 and 480.00 and 33,000.00 for bicluster_0555 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score Re-Annotated Start Tuberculist Annotated Start
-0.902 2010746 2010656
Last update: 10/16/2017 - 11:42
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14896 MT1827 192
Product (LegacyBRC) Product (RefSeq)
Putative cytochrome P450 144 cytochrome p450 144 CYP144
Operon # Operon
1161
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Bisphenol degradation

35
Total items in this category:  

KEGG

Polycyclic aromatic hydrocarbon degradation

47
Total items in this category:  

KEGG

Aminobenzoate degradation

73
Total items in this category:  

KEGG

Limonene and pinene degradation

65
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15608915 NP_216293.1 Run
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.010000 0.56

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: