Rv2332 NAD-dependent malic enzyme (EC


Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2332 mez NAD-dependent malic enzyme (EC CDS 2605108 2606754 + 1 647 548 FALSE

Rv2332 (NAD-dependent malic enzyme (EC is predicted to be co-regulated in modules bicluster_0284 with residual 0.42 and bicluster_0411 with residual 0.41.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 2,400.00 and 7,700.00 for bicluster_0284 and 0.02 and 36.00 for bicluster_0411 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 13:07
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-15723 MT2394 1858
Product (LegacyBRC) Product (RefSeq)
Putative malate oxidoreductase [NAD] malate dehydrogenase
Operon # Operon
1537 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics


Malate dehydrogenase (oxaloacetate-decarboxylating) Pyruvate metabolism
Locus Tuberculist Genome View


Locus Tag KEGG Pathways


Pyruvate metabolism

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Two-component system

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BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network


GI Number Protein ID Blast Conserved Domains
57116971 NP_216848.2 Run

malate dehydrogenase (oxaloacetate-decarboxylating) activity

malate dehydrogenase (oxaloacetate-decarboxylating) activity

Catalysis of the reaction: (S)-malate + NAD+ = pyruvate + CO2 + NADH + H+. Also decarboxylates oxaloacetate.
GO Category: 
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The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.320000 1.45

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: