Rv2503c Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit B (EC


Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv2503c scoB Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit B (EC CDS 2818471 2819127 - 657 218 FALSE

Rv2503c (Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit B (EC is predicted to be co-regulated in modules bicluster_0240 with residual 0.52 and bicluster_0530 with residual 0.56.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 5.80 for bicluster_0240 and 0.00 and 0.46 for bicluster_0530 respectively.

These modules are enriched for following go terms: fatty-acyl-CoA synthase activity, C-acyltransferase activity .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 13:57
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18508 MT2578 2423
Product (LegacyBRC) Product (RefSeq)
Probable succinyl-CoA:3-ketoacid-coenzyme A transferase subunit B succinyl-CoA:3-ketoacid-coenzyme A transferase subunit beta ScoB
Operon # Operon
1645 - - - - - -
Locus Tuberculist Genome View


Locus Tag KEGG Pathways


Synthesis and degradation of ketone bodies

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Valine, leucine and isoleucine degradation

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Butanoate metabolism

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BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network


GI Number Protein ID Blast Conserved Domains
15609640 NP_217019.1 Run

3-oxoacid CoA-transferase activity

3-oxoacid CoA-transferase activity

Catalysis of the reaction: succinyl-CoA + a 3-oxo acid = succinate + a 3-oxo-acyl-CoA.
GO Category: 
Total items in this category:  

response to hypoxia

response to hypoxia

Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating lowered oxygen tension. Hypoxia, defined as a decline in O2 levels below normoxic levels of 20.8 - 20.95%, results in metabolic adaptation at both the cellular and organismal level.
GO Category: 
Total items in this category:  
No TFOE experiment results were found

Quantitative Proteomics Data

t-test p-value Cholesterol/Glycerol Ratio
0.890000 1.98

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: